Simultaneous determination of pyrimethamine and sulphadoxine in human plasma by high-performance liquid chromatography after automated liquid-solid extraction

Astier, Hélène; Renard, Christophe; Cheminel, V.; Soares, Olivier; Mounier, C.; Peyron, François; Chaulet, J. F.

doi:10.1016/s0378-4347(97)00264-8

Cited by 37 publications

(15 citation statements)

References 5 publications

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“…Previous HPLC-based techniques for measuring pyrimethamine and sulfadiazine plasma levels require 400 ll of whole blood, which is difficult to collect from infants except by venous puncture [1,25], and a new method for the simultaneous determination of pyrimethamine, sulfadiazine and its metabolite, N-acetylsulfadiazine, using only 25 ll of whole blood, was introduced in this study [12].…”

Section: Discussionmentioning

confidence: 99%

Treatment of infants with congenital toxoplasmosis: tolerability and plasma concentrations of sulfadiazine and pyrimethamine

Schmidt

Høgh

Andersen³

et al. 2005

Eur J Pediatr

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The aim was to study the tolerability and plasma concentrations of pyrimethamine and sulfadiazine in children treated for congenital toxoplasmosis. Infants were diagnosed through the Danish Toxoplasma Neonatal Screening Programme, based on detection of toxoplasma-specific IgM- and/or IgA-antibodies on 3 mm blood spots collected from phenylketonuria [PKU cards (Guthrie cards)]. Toxoplasma-infected children received 3 months' continuous treatment with 50-100 mg/kg per day sulfadiazine in two separate administrations and 1 mg/kg per day pyrimethamine after a 1-day loading dose of 2 mg/kg, and folinic acid 7.5 mg was administered twice weekly. Blood cell counts and body weight were recorded during follow-up. The plasma concentrations of pyrimethamine and sulfadiazine were analysed in a subgroup of seven children, using high performance liquid chromatography with ultraviolet and mass spectrometric detection. Of 48 infants, 41 completed the treatment without change in schedule. Six infants had neutrophil counts below 0.5x10(9)/l, and one infant had an elevated bilirubin value. Twenty-nine children were tested by a series of neutrophil counts during treatment. The neutrophil count was

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Section: Discussionmentioning

confidence: 99%

Treatment of infants with congenital toxoplasmosis: tolerability and plasma concentrations of sulfadiazine and pyrimethamine

Schmidt

Høgh

Andersen³

et al. 2005

Eur J Pediatr

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“…There are few methods reported in the literature for analysis of pyrimethamine & sulphadoxine alone or in combination with other drugs in the pure form, pharmaceuticals formulations and biological fluids by UVspectrophotometer (Onah and Odeiani, 2002, Meena and . Sandhya, 2013), HPTLC (Meena and Sandhya, 2013), Capillary zone electrophoresis (Amin et al, 2012), RP-HPLC (Green et al, 2002, Bergqvist et al, 1991Astier et al, 1997;Bergqvist et al, 1985;SaeedArayne et al, 2010), LC-MS (Sinnaeve et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Pyrimethamine and Sulphadoxine in Bulk and Tablet Dosage Form

Kumar¹,

Sindgi²,

Satla³

et al. 2016

J App Pharm Sci

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A stability indicating simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the combined tablet formulation of pyrimethamine & sulphadoxine. Chromatographic separation was optimized by gradient HPLC on a C18 column [Inertsil Silica, 250 x 4.6 mm, 5µ] utilizing a mobile phase of potassium dihydrogen phosphate and acetonitrile taken in the ratio 70:30 at a flow rate of 1.0 ml/min with UV detection at 221nm. The retention time of pyrimethamine and sulphadoxine was 2.77 and 6.57 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation, robustness and stress degradation studies. Validation of the method was done in accordance with ICH guidelines for the assay of active ingredients. Thus validated method can be recommended for the routine laboratory analysis.

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“…In some cases gastrointestinal hemorrhage and dyspepsia are reported. Hypersensitivity reactions occur occasionally, including skin rash, which can be controlled upon immediate withdrawal of therapy (British Pharmacopoeia, 2003;Merck, 2001;Martindale and Westcott, 1996) A number of methods have been reported by various authors for the determination of mefloquine (Lamant et al, 1988;Bergqvist et al, 1988Bergqvist et al, , 1991Bergqvist et al, , 1993Bergqvist et al, , 1994Karbwang et al, 1989;Qiu et al, 1992;Souri et al, 1997;Green et al, 1999), pyrimethamine (Timm and Weidekamm, 1982;Bergqvist et al, 1991;Eljaschewitsch et al, 1996;Bangchang et al, 1997;Astier et al, 1997;Liboux et al, 1991;Zytkovicz et al, 1991;Tracqui et al, 1993;Roberts et al, 1995;Gong et al, 2002;Lin and Jeng, 2002), sulfadoxine (Bhoir et al, 2001;Bonini et al, 1981), and ibuprofen (Sochor et al, 1994(Sochor et al, , 1995Lemko et al, 1993;Castillo and Smith 1993;Rifai et al, 1996;Save et al, 1997;Kepp et al, 1997;Bhushan and Martens, 1998;Kang et al, 1998;Ducret et al, 1998;Gasco-Lopez et al, 1999;Canaparo et al, 2000;Vermeulen and Remon, 2000;…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of pyrimethamine, sulfadoxine, mefloquine, and ibuprofen in pharmaceutical formulations by RP-HPLC

et al. 2009

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A simple and rapid high-performance liquid chromatographic (HPLC) method for simultaneous determination and quantification of pyramethamine, sulfadoxine, mefloquine HCl and ibuprofen was developed. The chromatographic system consisted of a Shimadzu LC-10 AT VP pump, SPD-10 AV VP UV visible detector, and CBM-102 Bus Module integrator. Separation was achieved on a lBondapak 125 A, C-18, 10-lm column at room temperature. The sample was introduced through an injector valve with a 10-lL sample loop. Acetonitrile/water (50:50, v/v) was used as the mobile phase, with a flow rate of 2 mL/min. The pH was adjusted to 2.6 with phosphoric acid. UV detection was performed at 220 nm. The results obtained showed good agreement with the declared content. Recovery values were from 99.43 to 101.52% for mefloquine (250 mg in Fansimef Ò tablet), from 99.32 to 100.7% for pyrimethamine (25 mg in Fansimef Ò tablet), from 99.29 to 100.21% for sulfadoxine (500 mg in Fansimef Ò tablet), and from 99.96 to 100.04% for ibuprofen (400 mg Dolofen Ò tablet). The proposed method is rapid, accurate, and selective; it may be used for quantitative analysis of pyramethamine, sulfadoxine, mefloquine HCl, and ibuprofen from raw materials, in bulk drugs, and from dosage formulations.

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Simultaneous determination of pyrimethamine and sulphadoxine in human plasma by high-performance liquid chromatography after automated liquid-solid extraction

Cited by 37 publications

References 5 publications

Treatment of infants with congenital toxoplasmosis: tolerability and plasma concentrations of sulfadiazine and pyrimethamine

Treatment of infants with congenital toxoplasmosis: tolerability and plasma concentrations of sulfadiazine and pyrimethamine

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Pyrimethamine and Sulphadoxine in Bulk and Tablet Dosage Form

Simultaneous determination of pyrimethamine, sulfadoxine, mefloquine, and ibuprofen in pharmaceutical formulations by RP-HPLC

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