Simultaneous determination of polyamines and acetylpolyamines in human urine by capillary electrophoresis with fluorescence detection

Abstract

There has been evidence linking elevated polyamines (PAs) and acetylpolamines (AcPAs) level and cancer. So the simultaneous analysis of these compounds has become important task for cancer diagnosis and antitumor drug monitoring. A simple, fast and inexpensive CZE-LIF method has been developed for the determination of cadaverine (CAD), putrescine (PUT), spermine (SPM), spermidine (SPD), acetylspermine (ASPM), and acetylspermidine (ASPD) in human urine using 4-chloro-7-nitro-2,1,3-benzooxadiazole as a fluorescent reagent. Labeling reaction conditions were systematically investigated and were found to be 20 mM borate buffer at pH 7.4, labeling reaction time, and temperature were 10 min and 70°C, respectively. Under these optimized conditions the four PAs, two AcPAs and the internal standard were separated in 6 min. An Exactive-MS with an ESI source was used for identification of the bis-derivative of the ASPM. The method was validated in term of linearity, LODs, repeatability, intra- and interday assays, recovery, and selectivity. The LODs for CAD, PUT, SPM, SPD, ASPM, and ASPD were found to be 7.6, 10.0, 9.0, 8.8,7.8, and 3.3 nM, respectively. The method was successfully applied for the analysis of PAs and AcPAs in healthy human urine samples.

Carbon Dots and a CdTe Quantum Dot Hybrid-Based Fluorometric Probe for Spermine Detection

“…1 The sensitive and selective detection of spermine is crucial to evaluate the safety and quality of meat products during their storage and transportation. 2 Traditional analytical techniques including mass spectrometry, 3 chromatography, 4 and electrophoresis 5 suffer from the tedious pretreatment procedures and high cost. Thus, it is urgent to develop efficient approaches to detect spermine with low cost and sensitivity.…”

Simple fluorescence optosensing probe for spermine based on ciprofloxacin-Tb3+ complexation

“…However, its low volatility, low molecular weight, and the absence of chromophores significantly impede the sensitivity and selectivity for SP detection [17][18][19][20]. Conventional methods for the quantitative determination of spermine, such as chromatographic techniques [21][22][23], immunoassays [24,25], electrophoresis [26][27][28], and electrochemical [29,30] have been developed, but the time consuming, skilled personnel, and high cost of related equipment significantly affect the popularity of these methods. In contrast, fluorescence methods have become a popular method due to the versatility, sensitivity, low detection, and visual capacities [31][32][33].…”

Utilization of 4-Chloro-7-Nittobenzo-2-Oxa-1, 3-Diazol (NBD-CL) as Chromogenic Reagent for Determination of Metformin hydrochloride (MET) in Pharmaceutical Formulation

“…However, some of these methods are complicated and time consuming, involve the use of large volume organic solvents and specific reagent and requirement of expensive instruments. NBD-CL has been proved to be useful and sensitive analytical derivatizing agent for spectrophotometric analysis of pharmaceutical bearing a primary or secondary amine group [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] . In depth review on the applications of NBD-CL for determination of pharmaceutical bearing amine group have been reviewed by Elbashir et al 36,37 .…”