Simultaneous Determination of Paracetamol, Aceclofenac and Chlorzoxazone in Pharmaceutical Dosage Form by UHPLC Method

Abstract

Aim: UHPLC method was developed and validated for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form.Methods: Separation was performed in Agilent technologies 1220 infinity LC system equipped with open lab CDS Chemstation software using Eclipse plus C18 (50 × 4.6 mm id, 1.8 µm) column with mobile phase containing 0.05% orthophosphoric acid, adjusted to pH 6.0 with triethylamine and acetonitrile in the ratio of 70:30 v/v with a flow rate of 0.5 mL/min and detection wavelength 270 nm. Results:The method was found to be linear in the concentration range of 161.59-484.77 µg/mL for paracetamol, 49.69-149.09 µg/mL for aceclofenac and 124.81-374.43 µg/mL for chlorzoxazone with correlation coefficient of 0.999 for all the three drugs respectively. The %RSD of 0.57, 0.45 and 0.60 for intra-day and 0.96, 0.88 and 0.84 for inter-day precision, respectively for of Paracetamol, Aceclofenac and Chlorzoxazone suggest the precision of the method as all these values are less than 2%. The method has shown good, consistent recoveries for of Paracetamol (99.90-99.95%), Aceclofenac (99.83-99.92%) and Chlorzoxazone (99.39-99.59%) which are close to 100%. Conclusion:The method was found to be accurate, precise, specific, robust and linear for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form.

Simultaneous Detection of Chlorzoxazone and Paracetamol Using a Greener Reverse-Phase HPTLC-UV Method

“…Ultra-high-performance liquid chromatography (UHPLC) and supercritical fluid chromatography methods have also been utilized for simultaneous detection of CZN and PCT in commercially available combined dosage forms [21,22]. UHPLC and liquid chromatography tandem mass-spectrometry methods have also been used for simultaneous detection of CZN and PCT in human plasma samples [23,24].…”

“…For simultaneous detection of CZN and PCT in various commercially available combined pharmaceutical preparations, a number of "high-performance liquid chromatography (HPLC)" methods are also reported [13][14][15][16][17][18][19][20]. Ultra-high-performance liquid chromatography (UHPLC) and supercritical fluid chromatography methods have also been utilized for simultaneous detection of CZN and PCT in commercially available combined dosage forms [21,22]. UHPLC and liquid chromatography tandem mass-spectrometry methods have also been used for simultaneous detection of CZN and PCT in human plasma samples [23,24].…”

Electrocoagulation Technique for Cephalothin Sodium Removal with Effective Sludge Management

“…CS is the sodium salt of cephalothin, a semisynthetic beta-lactam, first-generation cephalosporin antibiotic with an empirical formula of C 6 H 15 N 2 NaO 6 S 2 and molecular weight of 418.4 g mol -1 . The excessive use of the untreated traces of antibiotics can result in adverse effects on living beings [2]. The micropollutants present in the wastewater are not completely removed by conventional treatment processes [3,4].…”