Simultaneous determination of paeoniflorin from total glucosides of paeony in Sprague–Dawley rats and spontaneously hypertensive rats by high‐performance liquid chromatography–tandem mass spectrometry: <i>in vivo</i> and <i>in vitro</i> studies

Xiong, Shanbai; Wang, Yuyun

doi:10.1002/bmc.3751

Cited by 17 publications

(10 citation statements)

References 21 publications

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“…Blood samples (~0.1 ml) were collected from jugular vein of rats at pre‐dose (0), 5, 15, 30, 60, 120, 240, 360, 480, 720 and 1440 min. The blood samples were immediately centrifuged at 4500 rpm for 10 min at 4°C and the plasma samples were transferred carefully from each collection and stored at −20°C until analysis (Li et al, ; Xiong & Wang, ).…”

Section: Methodsmentioning

confidence: 99%

Development and full validation of an LC–MS/MS methodology to quantify capmatinib (INC280) following intragastric administration to rats

Fan

Yang

Cui

et al. 2020

Biomedical Chromatography

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A highly sensitive, specific and simple LC–MS/MS method for quantification of capmatinib (INC280) in rat plasma was presented. The LC–MS/MS method was validated in terms of specificity and selectivity, linearity, accuracy and precision, matrix effect, extraction recovery, dilution integrity, carryover and stability as per the US Food and Drug Administration's bioanalytical method validation guideline. The validated assay was applied for quantification of capmatinib from a pharmacokinetic study in rats following oral administration at the doses of 1.0, 3.0 and 9.0 mg/kg. The calibration curve ranges from 1 to 2000 ng/ml with desirable linearity and r2 > 0.99. The intra‐ and inter‐batch accuracies were within 99.24–103.59 and 97.76–102.83% with coefficients of variation 5.08–7.36 and 3.18–4.99%, respectively. No significant interference was observed by endogenous peak at the retention time of capmatinib and IS. The assay was free from any matrix effect and showed precise recovery across the calibration curve range, and samples were stable under all experimental conditions. The validated assay was successfully applied to analyze plasma samples of pharmacokinetic study in rat to determine the concentration of capmatinib. In summary, a novel method for analyzing capmatinib in rat plasma has been successfully validated and is now being utilized for quantification of capmatinib from pre‐clinical studies.

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Section: Methodsmentioning

confidence: 99%

Development and full validation of an LC–MS/MS methodology to quantify capmatinib (INC280) following intragastric administration to rats

Fan

Yang

Cui

et al. 2020

Biomedical Chromatography

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“…Blood samples (approximately 0.1 ml) were collected from jugular vein of rats in heparinized tubes at 5, 15, 30, 60, 90, 120, 240, 360, 480, 720 and 1,440 min after dosing. The blood samples were immediately centrifuged at 1,280 g for 15 min at 4°C to obtain the plasma, which was then stored at −20°C until analysis (Li et al, 2019; Xiong & Wang, 2016).…”

Section: Methodsmentioning

confidence: 99%

Determination of selpercatinib, a RET kinase inhibitor, in rat plasma and its application to a pharmacokinetic study

Wang

Shi

Jin

et al. 2020

Biomedical Chromatography

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Selpercatinib (LOXO‐292) is a selective and potent RET inhibitor. A highly sensitive, rapid and specific high‐performance liquid chromatography with tandem mass spectrometry (LC–MS/MS) method for quantification of selpercatinib in rat plasma is reported. The method was validated in terms of selectivity, linearity, accuracy and precision, extraction recovery and matrix effect, stability and carryover as per the US Food and Drug Administration guidelines for bioanalytical method validation. Selpercatinib was detected by an electrospray ionization interface under selective reaction monitoring conditions in the positive ion mode. The calibration curve was linear over the concentration range from 1 to 2000 ng/ml with r2 = 0.9951. The intra‐ and inter‐batch accuracy values ranged from 97.45 to 100.97% and from 98.70 to 100.74% with coefficients of variation of 2.45–6.99% and 5.89–7.99%, respectively. The extraction recovery and IS‐normalized matrix factor were acceptable for the bioanalysis of selpercatinib. Additionally, selpercatinib was found to be stable under the detected conditions. It showed linear pharmacokinetic characteristics following oral administration to rats at 2.0–18.0 mg/kg. The results showed that the novel method for detecting selpercatinib in rat plasma could be successfully applied for quantification of selpercatinib in biosamples from nonclinical studies.

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“…Radix Paeoniae Alba (RPA), with paeoniflorin as principal bioactive component, is a traditional Chinese medicine that lowers blood pressure and exhibits anti-inflammatory and antioxidative properties, among others [ 8 ]. An increasing number of studies found that paeoniflorin or extract of RPA exerts a positive effect on cardiovascular function [ 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Beneficial Effects of Paeoniflorin Enriched Extract on Blood Pressure Variability and Target Organ Damage in Spontaneously Hypertensive Rats

Yang

Lei

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Blood pressure variability (BPV) is associated with the development and progression of severe target organ damage (TOD). This study aims to evaluate the protective effect of paeoniflorin enriched extract from Radix Paeoniae Alba (PG) on BPV and TOD in spontaneously hypertensive rats (SHR). All SHR were orally treated with distilled water, metoprolol (MP, 20 mg/kg), and PG (PG-H, 90 mg/kg or PG-L, 30 mg/kg) for a single time or daily for 7 weeks. The 24-hour dynamic blood pressure was monitored and then calculated BPV including long- and short-term systolic blood pressure variability (SBPV), diastolic blood pressure variability (DBPV), mean blood pressure variability (MBPV), and heart rate variability (HRV) as well as the 24-hour-SBP, 24-hour-DBP, and 24-hour-MBP. The protective effects of PG on TOD were observed by histopathologic and biochemical detection. The results indicated that long- and short-term SBPV, DBPV, MBPV, and HRV as well as 24-hour-SBP, 24-hour-DBP, and 24-hour-MBP showed no significant changes after single-dose administration of PG and significantly decreased after administration with PG for 7 weeks. PG could also markedly improve the damage of aorta, heart, kidney, and brain. This study suggested that PG could notably reduce BPV, stabilize blood pressure, and mitigate TOD in SHR.

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Simultaneous determination of paeoniflorin from total glucosides of paeony in Sprague–Dawley rats and spontaneously hypertensive rats by high‐performance liquid chromatography–tandem mass spectrometry: in vivo and in vitro studies

Cited by 17 publications

References 21 publications

Development and full validation of an LC–MS/MS methodology to quantify capmatinib (INC280) following intragastric administration to rats

Development and full validation of an LC–MS/MS methodology to quantify capmatinib (INC280) following intragastric administration to rats

Determination of selpercatinib, a RET kinase inhibitor, in rat plasma and its application to a pharmacokinetic study

Beneficial Effects of Paeoniflorin Enriched Extract on Blood Pressure Variability and Target Organ Damage in Spontaneously Hypertensive Rats

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