Simultaneous determination of nitroglycerin and its dinitrate metabolites by capillary gas chromatography with electron-capture detection

Simultaneous determination of nitroglycerin and dinitrate metabolites in metabolism studies using liquid chromatography–mass spectrometry with electrospray ionization

“…In metabolism studies of NTG, therefore, it is useful to monitor 1,2-GDN and 1,3-GDN concurrently with NTG in order to provide possible mechanistic insights. Presently, most investigators employ either thin layer chromatography-liquid scintillation spectrometry (TLC-LSS) [1,4,5] or gas chromatography (GC) with either electron capture detector or mass spectrometry [6][7][8][9] for these studies. The TLC-LSS method produces good resolution but suffers from low sensitivity [10] and lack of an internal standard, while the GC system requires solvent extraction and may be affected by problems such as thermal decomposition and adsorption [11].…”