Simultaneous determination of nifedipine and dehydronifedipine in human plasma by liquid chromatography–tandem mass spectrometry

Streel, Bruno; Zimmer, Collin; Sibenaler, R.; Ceccato, Attilio

doi:10.1016/s0378-4347(98)00423-x

Cited by 43 publications

(36 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Roosmemalen et al [22] Streel et al [25] Wang et al [26] Wang et al [27] Patel et al [28] Plasma volume (ml) …”

Section: Referencementioning

confidence: 99%

“…These methods include HPLC [8][9][10][11][12][13][14] coupled with UV detectors or electrochemical detector, but tedious sample processing technique, lengthy analysis time and lack of sensitivity were major limitations in these methods. Diverse detection techniques like electron-capture detection [15][16][17][18][19][20], nitrogen phosphorus detection [21] and mass spectrometric (MS) detection [22,23] were reported.…”

mentioning

confidence: 99%

“…Although Roosmemalen et al reported a method on estimation of polar metabolites of nifedipine, arresting ex vivo inter-conversion among metabolites and analyte [22] remained unaddressed. Reported LC-MS/MS methods showed marked limitations as these methods are not tuned to labile metabolite separation and arresting ex vivo degradation of nifedipine [25][26][27][28]. Role of dwell time in MS, special analytical precautions, use of appropriate anticoagulant along with detailed stability in diverse type of matrices remained unmet (Table 1).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Method Development Challenges and Regulatory Expectation in Nifedipine

Goswami

Gurule

Singh

et al. 2016

Int. J. Pharmacokinet.

View full text Add to dashboard Cite

Aim: A novel and accurate high-throughput tandem mass spectroscopic method has been developed and validated for determination of nifedipine, a calcium-channel blocker. Materials & methods: Nifedipine was detected on a triple quadruple tandem mass spectrometer by multiple reactions monitoring mode via ESI source. Results: The selective and sensitive method was linear in the concentration range of 0.501 to 360.100 ng/ml and reported no matrix effect. The percent accuracy of the method was found to be between 99.7 and 102.80%, while percentage of RSD was in range of 1.77 to 4.73%. Conclusion: Validated method was used for bioavailability studies under fasting and fed conditions. Clinical pharmacokinetics of noncommunicable disease such as hypertension has interested the academicians and practitioners alike due to intricacies and limited knowledge of labile metabolites. Calciumchannel blocker drug slows down the progression of coronary artery calcification. Nifedipine (1,4-dihydro-2, 6-dimethyl-4-(2-nitrophenyl)-3,5-pyridine dicarboxylic acid dimethyl ester), belonging to the group of 1,4-dihydropyridines is one of the highly prescribed calcium-channel blockers that selectively dilates peripheral arteries [1]. It is widely used in the treatment of cardiovascular diseases such as hypertension, angina pectoris and Raynaud's phenomenon [2][3][4]. It inhibits the influx of extracellular calcium through myocardial and vascular membrane pores as well as restricts contractile processes of smooth muscle cells thereby decreasing total peripheral resistance and systemic blood pressure [5,6]. However, developing a robust method for analysis of Nifedipine in plasma is highly challenging due to multiple reasons. Nifedipine is a photo-labile compound, undergoing oxidative biotransformation in human body into pharmacologically inactive metabolites, dehydronifedipine and hydroxydehydro nifedipine carboxylate [7].In recent past, several analytical methods were reported for the determination of nifedipine in biological matrix. These methods include HPLC [8][9][10][11][12][13][14] coupled with UV detectors or electrochemical detector, but tedious sample processing technique, lengthy analysis time and lack of sensitivity were major limitations in these methods. Diverse detection techniques like electron-capture detection [15][16][17][18][19][20], nitrogen phosphorus detection [21] and mass spectrometric (MS) detection [22,23] were reported. However, scope of thermal decomposition of nifedipine under gas chromatography conditions was a co ncern and MS methods are usually preferred.Nifedipine is metabolized by cytochrome P-450 IIIA4 enzymatic activity, in vivo. Nifedipine metabolism involves oxidative dehydrogenation to dehydronifedipine, followed

show abstract

“…Roosmemalen et al [22] Streel et al [25] Wang et al [26] Wang et al [27] Patel et al [28] Plasma volume (ml) …”

Section: Referencementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Method Development Challenges and Regulatory Expectation in Nifedipine

Goswami

Gurule

Singh

et al. 2016

Int. J. Pharmacokinet.

View full text Add to dashboard Cite

show abstract

“…Among them ASPEC (Gilson), PrepStation (Hewlett-Packard) and Benchmate or Rapid trace (Zymark) devices have been commonly used in the last decade. 16,17 More recently, direct coupling of (i) turbulent flow chromatography with automated SPE in 96-well plates, (ii) ADS (alkyl-diolsilica)-restricted access phase or (iii) a sample SPE system such as Prospekt (Spark-Holland) with LC allows the development of fully on-line analysis. 17 -19 Numerous advantages accrue, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…16,17 More recently, direct coupling of (i) turbulent flow chromatography with automated SPE in 96-well plates, (ii) ADS (alkyl-diolsilica)-restricted access phase or (iii) a sample SPE system such as Prospekt (Spark-Holland) with LC allows the development of fully on-line analysis. 17 -19 Numerous advantages accrue, e.g. reduced sample handling and briefer exposure of the sample to variations in laboratory conditions such as light and temperature.…”

Section: Introductionmentioning

confidence: 99%

Fully automated determination of eserineN-oxide in human plasma using on-line solid-phase extraction with liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Pruvost

Ragueneau

Ferry³

et al. 2000

J. Mass Spectrom.

View full text Add to dashboard Cite

Liquid chromatography-mass spectrometry in the study of the metabolism of drugs and other xenobiotics

Oliveira

Watson

2000

Biomed. Chromatogr.

View full text Add to dashboard Cite

The application of liquid chromatography-mass spectrometry (LC/MS) to the study of metabolism of drugs and other xenobiotics is reviewed. Original research papers covering the period from 1998 to early 2000 and concerning the use of LC/MS in the study of xenobiotic metabolism in humans and other mammalian species are reviewed. LC/MS interfaces, sample preparation steps, column types, mobile phases and additives, and the type of metabolites detected are summarized and discussed in an attempt to identify the current and future trends in the use of LC/MS for metabolism studies. Applications are listed according to the parent xenobiotic type and include substances used in therapeutics, drug candidates, compounds being evaluated in clinical trials, environmental pollutants, adulterants and naturally occurring substances.

show abstract

Simultaneous determination of nifedipine and dehydronifedipine in human plasma by liquid chromatography–tandem mass spectrometry

Cited by 43 publications

References 42 publications

Method Development Challenges and Regulatory Expectation in Nifedipine

Method Development Challenges and Regulatory Expectation in Nifedipine

Fully automated determination of eserineN-oxide in human plasma using on-line solid-phase extraction with liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Liquid chromatography-mass spectrometry in the study of the metabolism of drugs and other xenobiotics

Contact Info

Product

Resources

About