Fully automated determination of eserineN-oxide in human plasma using on-line solid-phase extraction with liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Pruvost, Alain; Ragueneau, Isabelle; Ferry, A E; Jaillon, Patrice; Grognet, Jean‐Marc; Bénech, Henri

doi:10.1002/(sici)1096-9888(200005)35:5<625::aid-jms988>3.0.co;2-6

Cited by 14 publications

(12 citation statements)

References 33 publications

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“…A wide variety of research on the topic of rapid or high-throughput quantitation has produced a variety of methods. Examples range from automated liquid-liquid [71,72], solid-phase [73,74], direct injection (so-called "dilute and shoot") [75], and on-line extraction using a restricted access media column [76]. In fact, several recent reviews have been written which discuss the relative merits of these on-line/off-line high throughput extraction and mass spectrometry techniques as well as their impact on pharmacokinetic (PK) studies performed in drug discovery [77][78][79][80].…”

Section: Plasma Quantitation Methodsmentioning

confidence: 99%

Determination of rat oral bioavailability of soy-derived phytoestrogens using an automated on-column extraction procedure and electrospray tandem mass spectrometry

Mallis¹,

Sarkahian²,

Harris³

et al. 2003

Journal of Chromatography B

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Section: Plasma Quantitation Methodsmentioning

confidence: 99%

Determination of rat oral bioavailability of soy-derived phytoestrogens using an automated on-column extraction procedure and electrospray tandem mass spectrometry

Mallis¹,

Sarkahian²,

Harris³

et al. 2003

Journal of Chromatography B

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“…In order to obtain samples which are clean enough to enter the mass spectrometer, various sample preparation methods have already been applied [12][13][14][15][16]. As compared to liquid-liquid extraction, protein precipitation, and off-line SPE, on-line SPE offers advantage in term of being easier to handle and more time-efficient [17][18][19][20][21][22][23]. To extend the life of SPE extraction column, a simple protein precipitation with acetonitrile was applied prior to injection.…”

Section: Development Of the On-line Spe Proceduresmentioning

confidence: 99%

Quantitative determination of salidroside in rat plasma by on-line solid-phase extraction integrated with high-performance liquid chromatography/electrospray ionization tandem mass spectrometry

Chang

Yao

Hsieh

et al. 2007

Journal of Chromatography B

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“…Moreover, MALDI‐MS is relatively difficult to couple on‐line with sample pretreatment and separation techniques, and is not readily amenable to automation. ESI‐MS, on the other hand, allows on‐line detection to be combined with sample purification, concentration, and separation techniques such as microdialysis, solid phase extraction, liquid chromatography, and capillary electrophoresis (CE) 4–8. Thus, ESI‐MS is a powerful tool for the analysis of complex systems.…”

Section: Introductionmentioning

confidence: 99%

Identification of microbial mixtures by LC‐selective proteotypic‐peptide analysis(SPA)

2006

J. Mass Spectrom.

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This paper describes a method--using a combination of LC-MS/MS of selected bacteria-specific peptides and database search--for determining the species of bacteria present in a mixture. We identified the proteotypic peptides that were associated with specific bacteria by searching protein databases for the LC-MS/MS data. The retention time windows for specific peptide markers were used as an extra constraint so that the peptide markers of many bacterial species could be analyzed in a single LC-selective proteotypic-peptide analysis (SPA). We performed LC-MS/MS analyses on the proteolytic digest of cell extracts and monitored only the selected marker peptide ions at given elution time windows. The corresponding bacterial species could be characterized when the selected peptides that eluted at expected elution windows were identified correctly from the database. We managed to identify up to eight bacterial species simultaneously during a single LC-MS/MS analysis, as well as bacteria mixed in various abundances. Two marker ions having similar values of m/z, but obtained from two different bacterial samples, which would otherwise be selected as precursors within mass tolerance and would complicate the MS/MS data, were time-resolved using LC and then used to correctly identify their bacterial sources. The coupling of selective MS/MS monitoring with separation methods, such as LC, provides a highly selective and accurate analytical method for characterizing complex mixtures of bacterial species.

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Fully automated determination of eserineN-oxide in human plasma using on-line solid-phase extraction with liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Cited by 14 publications

References 33 publications

Determination of rat oral bioavailability of soy-derived phytoestrogens using an automated on-column extraction procedure and electrospray tandem mass spectrometry

Determination of rat oral bioavailability of soy-derived phytoestrogens using an automated on-column extraction procedure and electrospray tandem mass spectrometry

Quantitative determination of salidroside in rat plasma by on-line solid-phase extraction integrated with high-performance liquid chromatography/electrospray ionization tandem mass spectrometry

Identification of microbial mixtures by LC‐selective proteotypic‐peptide analysis(SPA)

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