Simultaneous Determination of Multiple Phytohormones in Plant Extracts by Liquid Chromatography−Electrospray Tandem Mass Spectrometry

Durgbanshi, Abhi̧lasha; Arbona, Vicent; Pozo, Óscar J.; Miersch, Otto; Sancho, Juan V.

doi:10.1021/jf050884b

Cited by 275 publications

(197 citation statements)

References 29 publications

(34 reference statements)

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“…This technique has been used to simultaneously determine the levels of different plant hormones, including IAA. The addition of labelled deuterium before extraction improves the technique precision, significantly reducing the time required for sample preparation and hormone analysis [118]. Capillary electrophoresis (CE) is another technique that has been used for auxin purification.…”

Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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Qualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees.

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Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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“…Liquid chromatography (LC) coupled to electrospray ionization (ESI) and tandem mass spectrometry (MS/MS) is a selective and sensitive tool for the trace analysis of multiple compounds in various matrices. Methods have been developed using LC-MS/MS to quantify melatonin [13], serotonin [13][14][15], HIAA [16,17], IAA [13,[18][19][20], and 3-IS [21]. Amirkhani et al [22] described a method to determine Trp, KYN, and KA in human plasma by capillary LC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography–tandem mass spectrometry

et al. 2011

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A sensitive, selective, and comprehensive method for the quantitative determination of tryptophan and 18 of its key metabolites in serum, urine, and cell culture supernatants was developed. The analytes were separated on a C18 silica column by reversed-phase liquid chromatography and detected by electrospray ionization tandem mass spectrometry in positive ion multiple reaction monitoring (MRM) mode, except for indoxyl sulfate which was measured in negative ion MRM mode in a separate run. The limits of detection and lower limits of quantification were in the range of 0.1-50 and 0.5-100 nM, respectively. Fully 13 C isotope-labeled and deuterated internal standards were used to achieve accurate quantification. The applicability of the method to analyze serum, urine, and cell culture supernatants was demonstrated by recovery experiments and the evaluation of matrix effects. Precision for the analysis of serum, urine, and cell culture supernatants ranged between 1.3% and 16.0%, 1.5% and 13.5%, and 1.0% and 17.4%, respectively. The method was applied to analyze changes in tryptophan metabolism in cell culture supernatants from IFN-γ-treated monocytes and immature or mature dendritic cells.

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“…Plant hormones were analyzed by HPLC coupled to tandem mass spectrometry as described in Durgbanshi et al (2005). Plant tissue was extracted in ultrapure water using a tissue homogenizer (Ultra-Turrax, Ika-Werke, Staufen, Germany).…”

Section: Sa Quantificationmentioning

confidence: 99%

Differential expression of gibberellin 20 oxidase gene induced by abiotic stresses in Zoysiagrass (Zoysia japonica)

Dai

Cheng

et al. 2012

Biologia

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The gibberellin 20-oxidase gene (GA20) plays an important role in plant growth and development. Differential expression of Zoysiagrass (Zoysia japonica Steud.) gibberellin 20 oxidase gene (ZjGA20) induced by abiotic stresses has not been reported. In this investigation, we first reported the differential expression of ZjGA20 in different Z. japonica tissues including root, young leaf, senescent leaf, blade, sheath, and stolon, as well as differential expression induced by abiotic stresses including low temperature (4 • C), H2O2(8 µM), salt stress (250 mM NaCl), 25% PEG6000, and high temperature (42 • C) by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Higher expression of ZjGA20 was observed in young leaf and sheath, compared to root, senescent leaf, blade, and stolon. Among different abiotic stresses, expression of ZjGA20 decreased under low temperature, 25% PEG6000, and high temperature. The highest expression of ZjGA20 was obtained when plants were treated with 8 µM H2O2 for 10 h and with 250 mM NaCl for 5 h. The analysis of the MDA content, POD activity, and permeability of the plasma membrane demonstrated that application of exogenous GA3 recovered tissue damage derived from low temperature treatment. In addition, the expression of ZjGA20 increased under low temperature stress. These results demonstrated that expression of ZjGA20 was regulated by abiotic stresses and the damage derived from abiotic stresses could be rescued by exogenously applied plant growth regulator GA3. Further more, exogenous gibberellin and salicylic acid (SA) alleviated the growth inhibition and death of the seedlings under stresses. The SA content in the seedlings treated with 80 µM GA3 was far greater than the control (with H2O) and plants under stress treatments. These data suggest that exogenous addition of GA3 is able to counteract the inhibitory effects of these adverse environmental conditions in Zoysiagrass growth through modulation of SA biosynthesis. This is the first study of differential expression of gibberellin 20 oxidase gene and growth regulation of GA3 in Zoysiagrass under stresses.

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Simultaneous Determination of Multiple Phytohormones in Plant Extracts by Liquid Chromatography−Electrospray Tandem Mass Spectrometry

Cited by 275 publications

References 29 publications

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography–tandem mass spectrometry

Differential expression of gibberellin 20 oxidase gene induced by abiotic stresses in Zoysiagrass (Zoysia japonica)

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