Simultaneous Determination of Monosaccharides in Glycoproteins by Capillary Electrophoresis

Soga, Tomoyoshi; Heiger, David N.

doi:10.1006/abio.1998.2727

Cited by 81 publications

(57 citation statements)

References 20 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The experimental record of indirect UV detection of such mixture is in accordance with this prediction, as seen from Fig. 1 in [24]. It should be noticed that this tendency is the opposite of the conductivity signal.…”

Section: Systems With Partly Ionized Electrolytessupporting

confidence: 86%

Conductivity detection in capillary zone electrophoresis: Inspection by PeakMaster

et al. 2005

Self Cite

View full text Add to dashboard Cite

A simple rule stating that the signal in conductivity detection in capillary zone electrophoresis is proportional to the difference between the analyte mobility and mobility of the background electrolyte (BGE) co-ion is valid only for systems with fully ionized electrolytes. In zone electrophoresis systems with weak electrolytes both conductivity signal and electromigration dispersion of analyte peaks depend on the conductivity and pH effects. This allows optimization of the composition of BGEs to give a good conductivity signal of analytes while still keeping electromigration dispersion near zero, regardless of the injected amount of sample. The demands to achieve minimum electromigration dispersion and high sensitivity in conductivity detection can be accomplished at the same time. PeakMaster software is used for inspection of BGEs commonly used for separation of sugars (carbohydrates, saccharides) at highly alkaline pH. It is shown that the terms direct and indirect conductivity detection are misleading and should not be used.

show abstract

Section: Systems With Partly Ionized Electrolytessupporting

confidence: 86%

Conductivity detection in capillary zone electrophoresis: Inspection by PeakMaster

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…An indirect method was also attempted in the determination of acidic monosaccharides, including sialic acids, that used 2,6-pyridine carboxylic acid at pH 12.10 as the UV background ion and cetyltrimethylammonium bromide for electroosmotic flow (EOF) reversal [135]. The detection limits ranged from 20 to 70 mM and the method was easily applied to composition analysis of glycoproteins.…”

Section: Sialic Acid and Acid Glycoproteinmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

View full text Add to dashboard Cite

Investigation of effective biomarkers for cancers is currently a popular area of study in clinical and cancer researches, because it can potentially lead to pre-cancer screening or pre-cancer diagnosis and may provide useful information on cancer type and the disease's stage of progression. More and more biochemical or chemical fluid components of the human body such as urine, blood, and cerebrospinal fluid have been considered to contain biomarkers, which are useful in cancer researches, pre-cancer diagnosis, and cancer follow-ups during or after cancer treatment. Several modern analytical techniques, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and other separation techniques as well as hyphenated techniques, have been extensively used in study of cancer biomarkers. Among these techniques, CE is considered to be a highly efficient and practical analytical technique because of the small sample volume requirement and its wide separation versatility, ranging from small inorganic molecules to large biomolecules. This review discusses the latest developments involving biomarkers and their analysis by CE, including a discussion of instrumental conditions, method developments, and data analysis.

show abstract

“…Its pK a value is 13.5 and it can only be negatively charged at high pH [123]. Mannitol analysis has been widely explored for a variety of reasons using a broad range of techniques including photometry [124,125], chromatography [126][127][128], CE [52,129] and NMR [130].…”

Section: Mannitolmentioning

confidence: 99%

A Review of Extraction and Analysis: Methods for Studying Osmoregulants in Plants

Kalsoom¹,

Bennett²,

Boyce³

2016

J Chromatogr Sep Tech

View full text Add to dashboard Cite

Simultaneous Determination of Monosaccharides in Glycoproteins by Capillary Electrophoresis

Cited by 81 publications

References 20 publications

Conductivity detection in capillary zone electrophoresis: Inspection by PeakMaster

Conductivity detection in capillary zone electrophoresis: Inspection by PeakMaster

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

A Review of Extraction and Analysis: Methods for Studying Osmoregulants in Plants

Contact Info

Product

Resources

About