Simultaneous Determination of Metformin and Glyburide in Tablets by HPTLC

Quantitative Determination of Metformin Hydrochloride in Tablet Formulation Containing Croscarmellose Sodium as Disintegrant by HPLC and UV Spectrophotometry

“…The assay of metformin HCl in immediate release tablets is usually carried out by UV spectrophotometry as per British Pharmacopoeia (BP) and United States Pharmacopoeia (USP) while HPLC method of assay has been given in the USP monograph for extended release metformin tablets [5][6]. Some methods have been reported in the literature for the estimation of metformin HCl in the presence of other drugs in formulations [7][8][9][10][11][12][13][14][15][16][17][18][19].…”

Stability-indicating HPTLC method for simultaneous determination of nateglinide and metformin hydrochloride in pharmaceutical dosage form

“…Among the different mobile phase combinations employed, the mobile phase consisting of chloroform:ethyl acetate:acetic acid in the ratio of 4:6:0.1 v/v/v gave the best resolution with sharp well defined peaks with R f values of 0.80 ± 0.02 and 0.17 ± 0.02 for nateglinide and metformin hydrochloride, respectively. The use of acetic acid was found to be necessary for the elution of metformin from the plate due to the fact that it is a weak basic drug, thereby interacting with the unreacted silanol groups on silica stationary phases (Ghassempour et al, 2006). However, increasing the ratio of acetic acid was found to be deleterious and affected the peak shape of nateglinide.…”

“…Spectrophotometric techniques have also been reported for determination of nateglinide in bulk and tablet dosage forms (Jain et al, 2009;Rastogi et al, 2009). High performance liquid chromatography for metformin individually in human plasma (Amini et al, 2005;Yuen and Peh, 1998;Cheng and Chou, 2001) and in combination with other drugs in plasma and pharmaceutical dosage form (Ö nal, 2009;Zhang et al, 2007;Lakshmi et al, 2009), LC/MS analysis (Mistri et al, 2007), UV spectrophotometric quantitation methods in bulk and pharmaceutical formulation (Arayne et al, 2009) and HPTLC methods (Ghassempour et al, 2006) have been reported.…”

Validated HPTLC Method for Simultaneous Estimation of Metformin Hydrochloride, Atorvastatin and Glimepiride in Bulk Drug and Formulation

“…Literature review reveals that methods have been reported for analysis of MET, ATV and GLM by LC and HPTLC in combination with other drugs separately [4][5][6][7][8][9][10][11][12][13][14]. LC-MS-MS development and validation for simultaneous quantitation of MET, GLM and pioglitazone in human plasma have been reported [6].…”