A stability indicating high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of isoniazid and its related substances of isonicotinic acid and isonicotinamide in fixed dose combination of isoniazid and ethambutol HCl tablet. Inertsil, C-18, 250 × 4.6 mm, 5 µ µ µ µ HPLC column with a mobile phase of potassium dihydrogen phosphate buffer of pH 6.9 with a flow rate of 1.5 ml/min has been used for the estimation. The detection wavelength was set at 254 nm in the photodiode array detector. The response of the detector was linear in the range of 0.25 to 1500 µg/ml for isoniazid, 0.25 to 3.00 µg/ml for isonicotinic acid and 0.50 to 3.00 µg/ml for isonicotinamide. The limit of detection (LOD) values for isoniazid, isonicotinic acid and isonicotinamide were 0.083, 0.083 and 0.165 µg/ml, respectively. This validated stability indicating method may be used for the estimation of related substances of isoniazid in tablets.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.