Simultaneous determination of major type‐B trichothecenes and the de‐epoxy metabolite of deoxynivalenol in chicken tissues by <scp>HPLC</scp>–<scp>MS</scp>/<scp>MS</scp>

Xu, Lixiao; Zhang, Guijun; Guo, Chunna; Zhang, Yaping; Zhang, Yi; Zheng, Jianlong; Yang, Haicui; Yang, Dexue; He, Limin; Zeng, Zhenling; Fang, Binghu

doi:10.1002/jssc.201301014

Cited by 11 publications

(6 citation statements)

References 41 publications

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“…In addition to LLE and SPE techniques, some authors included a hexane deffation step to ensure the removal of fat components present in the matrix, which could interfere in the detection process. The extraction of DON, 3-ADON, 15-ADON, and DOM-1 from chicken samples including muscle, liver, kidney, and fat, was performed by EtOAc-LLE followed by hexane deffation and Oasis HLB cartridge [ 130 ]. The extraction of ZON, ZAN and their metabolites α-ZOL, β-ZOL, α-ZAL, and β-ZAL from bovine liver and muscle was performed by several steps including MeOH and EtOAc extraction, intercalated by repeated hexane defattion steps [ 131 ].…”

Section: Resultsmentioning

confidence: 99%

Studies on the Presence of Mycotoxins in Biological Samples: An Overview

Escrivá¹,

Font²,

Manyes³

et al. 2017

Toxins

105

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Mycotoxins are fungal secondary metabolites with bioaccumulation levels leading to their carry-over into animal fluids, organs, and tissues. As a consequence, mycotoxin determination in biological samples from humans and animals has been reported worldwide. Since most mycotoxins show toxic effects at low concentrations and considering the extremely low levels present in biological samples, the application of reliable detection methods is required. This review summarizes the information regarding the studies involving mycotoxin determination in biological samples over the last 10 years. Relevant data on extraction methodology, detection techniques, sample size, limits of detection, and quantitation are presented herein. Briefly, liquid-liquid extraction followed by LC-MS/MS determination was the most common technique. The most analyzed mycotoxin was ochratoxin A, followed by zearalenone and deoxynivalenol—including their metabolites, enniatins, fumonisins, aflatoxins, T-2 and HT-2 toxins. Moreover, the studies were classified by their purpose, mainly focused on the development of analytical methodologies, mycotoxin biomonitoring, and exposure assessment. The study of tissue distribution, bioaccumulation, carry-over, persistence and transference of mycotoxins, as well as, toxicokinetics and ADME (absorption, distribution, metabolism and excretion) were other proposed goals for biological sample analysis. Finally, an overview of risk assessment was discussed.

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Section: Resultsmentioning

confidence: 99%

Studies on the Presence of Mycotoxins in Biological Samples: An Overview

Escrivá¹,

Font²,

Manyes³

et al. 2017

Toxins

105

View full text Add to dashboard Cite

show abstract

“…For example, Makun et al showed that 85% of tested eggs were contaminated by DON at concentrations ranging from 0.6 to 17.9 ng/g [ 30 ]. Recently, Xu et al researched DON levels in chicken tissues from Guangzhou, China [ 47 ]. Among those samples ( n = 20), DON was present in one muscle (2.1 μg/kg) and two kidney (1.3–2.0 μg/kg) tissue samples.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Quantitative Risk Assessment of Twelve Mycotoxins in Eggs and Chicken Tissues in China

Wang

Zhang

Yan

et al. 2018

Toxins

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Aflatoxins (AFs), deoxynivalenols (DONs), and zearalenones (ZENs) are common mycotoxins that contaminate feedstuff, causing contamination of poultry products. In our study, these mycotoxins were quantified in 152 egg samples collected from markets in Jiangsu (JS), Zhejiang (ZJ), and Shanghai (SH) and in 70 chicken tissue samples (liver, heart, and gizzard) from ZJ in China. The main mycotoxins observed in egg samples were DON, 15-AcDON, and ZEN, although only ZEN family mycotoxins (ZEN, α-ZEL, β-ZEL, and α-ZAL) were detected in chicken tissues. Furthermore, for the first time, we assessed the health risks of exposure of three populations (children, adults, and elder adults) to DONs (DON, 3-AcDON, and 15-AcDON) and ZEN in eggs (from three different areas) and to ZEN in chicken tissues. We show that the mean dietary intake (DI) values and the 97.5th percentile DI values of DON and ZEN through egg ingestion were lower than the provisional maximum tolerable daily intake (PMTDI) (1 μg/kg body weight (BW)/day) for the three populations in the three geographical areas studied. However, eggs contaminated with high levels of DONs and ZEN contributed to a large proportion of the PMTDI of these mycotoxins, especially in children and elder adults. Although ZEN was highly detected in the chicken tissues, no significant health risk was observed.

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“…The results showed that the best sensitivity for the mycotoxins analysed was achieved with acetonitrile (mobile phase A) and 10 mM of AcNH 4 in water (mobile phase B).The composition finally chosen is very similar to that in Xu et al (2014), who analysed DON, 3-ADON,15-ADON and DOM-1 using methanol and 0.1% formic acid in water, or Simsek et al (2012), who analysed DON and DON-3-glucoside using 0.01% acetic acid in water and 0.01% acetic acid in acetonitrile, in bakery products.…”

Section: Mycotoxinmentioning

confidence: 98%

Thermal stability and kinetics of degradation of deoxynivalenol, deoxynivalenol conjugates and ochratoxin A during baking of wheat bakery products

et al. 2015

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The stability of deoxynivalenol (DON), deoxynivalenol-3-glucoside (DON-3-glucoside), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), de-epoxy-deoxynivalenol (DOM-1) and ochratoxin A (OTA) during thermal processing has been studied. Baking temperature, time and initial mycotoxin concentration in the raw materials were assayed as factors. An improved UPLC-MS/MS method to detect DON, DON-3-glucoside, 3-ADON, 15-ADON and DOM-1 in wheat baked products was developed in the present assay. The results highlighted the importance of temperature and time in mycotoxin stability in heat treatments. OTA is more stable than DON in a baking treatment. Interestingly, the DON-3-glucoside concentrations increased (>300%) under mild baking conditions. On the other hand, it was rapidly reduced under harsh conditions. The 3-ADON decreased during the heat treatment; while DOM-1 increased after the heating process. Finally, the data followed first order kinetics for analysed mycotoxins and thermal constant rates (k) were calculated. This parameter can be a useful tool for prediction of mycotoxin levels.

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Simultaneous determination of major type‐B trichothecenes and the de‐epoxy metabolite of deoxynivalenol in chicken tissues by HPLC–MS/MS

Cited by 11 publications

References 41 publications

Studies on the Presence of Mycotoxins in Biological Samples: An Overview

Studies on the Presence of Mycotoxins in Biological Samples: An Overview

Occurrence and Quantitative Risk Assessment of Twelve Mycotoxins in Eggs and Chicken Tissues in China

Thermal stability and kinetics of degradation of deoxynivalenol, deoxynivalenol conjugates and ochratoxin A during baking of wheat bakery products

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