Simultaneous determination of lactic acid and pyruvic acid in tissue and cell culture media by gas chromatography after in situ derivatization-ultrasound-assisted emulsification microextraction

Zhang, Hua-Yin; Tan, Xin; Kang, Kai; Wang, Wei; Lian, Kaoqi; Kang, Weijun

doi:10.1007/s00216-018-1502-z

Cited by 11 publications

(7 citation statements)

References 39 publications

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“…Therefore, the ions from other substances interfered with the detection and amplified the response signal of PA. 28 Lastly, the complex components of serum could react with each other, to produce unknown compounds. For example, lactate dehydrogenase can react with lactic acid to produce PA. 1 Hence, the response to PA may be increased because of the GC/MS system, the chemical property of PA, and the interference from the serum.…”

Section: Recovery Precision Lod and Loqmentioning

confidence: 99%

“…At present, a variety of analytic methods are available for the quantification of PA, such as colorimetric analysis, 4 capillary electrophoresis with amperometry detection, 5 voltammetry/ polarography, 6 and chromatography. 1 Compared with other methods, gas chromatography/mass spectrometry (GC/MS) has been widely used in the detection of volatile and semi-volatile compounds because of its sensitivity, specificity, and reliability. 7 Zhang et al reported that GC/MS had better performance in PA analysis after suitable derivatization.…”

Section: Introductionmentioning

confidence: 99%

“…7 Zhang et al reported that GC/MS had better performance in PA analysis after suitable derivatization. 1 PA is known to be unstable and it readily undergoes an aldol-like self-condensation/polymerization reaction. 8 Due to the complex composition of many biological samples, PA can readily react with confounding substances present in these samples, such as carboxylic acids, nitride, aldehyde, and carbonyl compounds.…”

Section: Introductionmentioning

confidence: 99%

“…Pyruvic acid (PA) plays a central role in the metabolism of carbohydrates, proteins, and fats as an intermediate compound. 1 The dysregulated metabolism of PA is related to the progression of several diseases, such as cancers and diabetes. 2 Currently, the underlying mechanism of the PA pathway has been deemed an opportunity for the safe and effective therapy of these diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with other methods, gas chromatography/mass spectrometry (GC/MS) has been widely used in the detection of volatile and semi‐volatile compounds because of its sensitivity, specificity, and reliability 7 . Zhang et al reported that GC/MS had better performance in PA analysis after suitable derivatization 1 …”

Section: Introductionmentioning

confidence: 99%

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A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

Zhao

Zhang

Guo

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale The matrix effect is tricky in gas chromatography/mass spectrometry (GC/MS) analyses. Although several methods have been proposed to solve this problem, the results were unsatisfactory. Even fewer studies have assessed the performance of corrective methods. Hence, our study focused on assessing several common corrective methods, and then proposed a new strategy to correct for the matrix effect in GC/MS analyses. Methods In GC/MS analyses, the internal standard method (ISM) was employed to overcome the matrix effect during the detection of pyruvic acid (PA) in serum samples from a healthy adult female. The accuracy of the ISM was evaluated by comparing it with the standard addition method (SAM). To employ the ISM‐SAM strategy, correction factors (CFs) were established by combining the ISM and the SAM based on different groups. The CFs were used to normalize data onto the results of subsequent analyses. Results When using the ISM to detect levels of PA, a serious bias is observed, thereby affecting the conclusions reached. In contrast, more reliable data can be obtained after normalizing results by undertaking the ISM‐SAM strategy. The feasibility of this strategy was verified by comparing it with the results of the SAM alone. The ISM‐SAM strategy was successfully applied to quantify the PA levels in healthy people and nephrotic syndrome patients. Conclusions Our results indicated that a false outcome was presented when only the ISM was used to adjust the data, and important information would be missed if the correction strategy was not carried out. Therefore, ISM‐SAM, as an available correction method, should be adapted to improve the reliability of research results.

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Section: Recovery Precision Lod and Loqmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

Zhao

Zhang

Guo

et al. 2021

Rapid Comm Mass Spectrometry

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A photonic crystal fiber–based fluorescence sensor for simultaneous and sensitive detection of lactic acid enantiomers

Chi

et al. 2022

Anal Bioanal Chem

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Graphical abstract A photonic crystal fiber (PCF)–based fluorescence sensor is developed for rapid and sensitive detection of lactic acid (LA) enantiomers in serum samples. The sensor is fabricated by chemical binding dual enzymes on the inner surface of the PCF with numerous pore structures and a large specific surface area, which is suitable to be utilized as an enzymatic reaction carrier. To achieve simultaneous detection of l -LA and d -LA, the PCF with an aldehyde-activated surface is cut into two separate pieces, one of which is coated with l -LDH/GPT enzymes and the other with d -LDH/GPT enzymes. By being connected and carefully aligned to each other by a suitable sleeve tube connector, the responses of both l -LA and d -LA sensors are determined by laser-induced flourescence (LIF) detection. With the aid of enzyme-linked catalytic reactions, the proposed PCF sensor can greatly improve the sensitivity and analysis speed for the detection of LA enantiomers. The PCF sensor exhibits a low limit of detection of 9.5 μM and 0.8 μM, and a wide linear range of 25–2000 μM and 2–400 μM for l -LA and d -LA, respectively. The sensor has been successfully applied to accurate determination of LA enantiomers in human serum with satisfactory reproducibility and stability. It is indicated that the present PCF sensors would be used as an attractive analytical platform for quantitative detection of trace-amount LA enantiomers in real biological samples, and thus would play a role in disease diagnosis and clinical monitoring in point-of-care testing. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03788-5.

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The current binomial Sonochemistry-Analytical Chemistry

Cairós¹,

González‐Sálamo²,

Hernández-Borges³

2020

Journal of Chromatography A

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Simultaneous determination of lactic acid and pyruvic acid in tissue and cell culture media by gas chromatography after in situ derivatization-ultrasound-assisted emulsification microextraction

Cited by 11 publications

References 39 publications

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

A novel ISM‐SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid

A photonic crystal fiber–based fluorescence sensor for simultaneous and sensitive detection of lactic acid enantiomers

The current binomial Sonochemistry-Analytical Chemistry

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