Simultaneous determination of honokiol and magnolol in Magnolia officinalis by liquid chromatography with tandem mass spectrometric detection

Abstract

An optimized high-performance liquid chromatographic method coupled with tandem mass spectrometric detection (LC-MS/MS) was developed for the simultaneous determination of honokiol and magnolol in Magnolia officinalis. Honokiol and magnolol were separated from the extracts using a reversed-phase C(18) column with a mobile phase consisted of acetonitrile and water (75:25, v/v) at a flow-rate of 0.8 mL/min. Selected reaction monitoring (SRM) mode was used for all sample quantification by the precursor-ion/product ion pair m/z 265 --> m/z 224 for honokiol and m/z 265 --> m/z 247 for magnolol. Validation data showed that this method has good linearity (r(2) > 0.995) over the concentration range of 0.0025-0.5 microg/mL for honokiol and magnolol, and both intra- and inter-day variability were acceptable within 15% at the lowest concentrations for this method. This proposed method provides excellent specificity, higher sensitivity and shorter run time than conventional methods and was applied successfully to determine the contents of honokiol and magnolol in M. officinalis.

Oral bioavailability of curcumin in rat and the herbal analysis from Curcuma longa by LC–MS/MS

“…The structures of curcumin and demethoxycurcumin were identified by spectroscopic analysis ( 1 H-, 13 C-and 2D-NMR, UV, and MS) and compared with data from the literature [17]. Internal standard honokiol was also purified from our lab [18]. The authentic curcuminoids and honokiol were dissolved in methanol at a concentration of 1 mg/ml in brown glass vials and stored at 4 • C. Liquid chromatographic grade solvents and reagents were obtained from Merck (Darmstadt, Germany).…”

Mesoporous silica-based electrochemical sensor for simultaneous determination of honokiol and magnolol

“…Until now, various methods, such as high-performance liquid chromatography (HPLC) [1,2], capillary electrophoresis [3,4], liquid chromatography with mass spectrometry [5], and synchronous fluorescence spectroscopy [6], were reported for the determination of honokiol and magnolol. From their molecular structures, it is very clear that honokiol and magnolol contain phenolic hydroxy group, which can be oxidized and exhibit electrochemical activity.…”

Polyethylene glycol-based ultrasound-assisted extraction of magnolol and honokiol from CortexMagnoliae Officinalis

“…It had been recorded earlier in the famous Compendium of Materia Medica and widely used to treat many diseases including anxiety and nervous disturbance, thrombotic stroke, typhoid fever and dead muscle (Tsai et al 1994;Wu et al 2006;Lin et al 2011). Besides this, Cortex Magnoliae Officinalis has been officially listed in the Chinese Pharmacopoeia (Pharmacopoeia Committee of China 2010) considering its great medicinal significance.…”