Polyethylene glycol-based ultrasound-assisted extraction of magnolol and honokiol from CortexMagnoliae Officinalis

Abstract

In this study, a kind of green solvent named polyethylene glycol (PEG) was developed for the ultrasound-assisted extraction (UAE) of magnolol and honokiol from Cortex Magnoliae Officinalis. The effects of PEG molecular weight, PEG concentration, sample size, pH, ultrasonic power and extraction time on the extraction of magnolol and honokiol were investigated to optimise the extraction conditions. Under the optimal extraction conditions, the PEG-based UAE supplied higher extraction efficiencies of magnolol and honokiol than the ethanol-based UAE and traditional ethanol-reflux extraction. Furthermore, the correlation coefficient (R(2)), repeatability (relative standard deviation, n = 6) and recovery confirmed the validation of the proposed extraction method, which were 0.9993-0.9996, 3.1-4.6% and 92.3-106.8%, respectively.

Rapid determination of multiple components in herbal medicine using a single reference compound by high‐performance liquid chromatography at equal absorption wavelength: Case study of Magnoliae officinalis cortex

“…cardiovascular modulation [8], inhibition of pathogenic bacteria, and inflammation [9, 10], Because of their good activity and high content, honokiol and magnolol were chosen as MOC quality markers in Chinese pharmacopoeia [11]. Several HPLC methods for the determination of honokiol and magnolol in MOC have been reported, including HPLC with ultraviolet detection (HPLC‐UV), HPLC with fluorescence detector, HPLC with electrochemical detection, and HPLC with mass spectrometry (HPLC‐MS) [12–17]. However, the reported HPLC procedures were time‐consuming and applied large volumes of harmful organic solvents.…”

An Ultra-rapid and Green Method for Simultaneous Determination of Honokiol and Magnolol in Magnoliae Officinalis Cortex with One Standard by HPLC-UV at Equal Absorption Wavelength

“…Several high-performance liquid chromatography (HPLC) methods for determination of honokiol and magnolol in MOC had been reported, such as HPLC with ultraviolet detection (HPLC-UV) (Ma et al, 2022;Zhang et al, 2014;He et al, 2015), HPLC with uorescence detector (HPLC-FLD) (Hu and Yin, 2015), HPLC with electrochemical detection (HPLC-ECD) (Yang et al, 2011) and HPLC with mass spectrometry (HPLC-MS) (Wu et al, 2006). However, the reported HPLC methods were time-consuming and took amounts of harmful organic solvents.…”

“…For example, in Chinese pharmacopoeia, the maceration extraction (ME) combined with HPLC-UV, which took more than 24 hours and consumed 60.5 mL methanol (Ma et al, 2022). The reported ultrasonic extraction (UE) and HPLC-UV method took 60 min and consumed 24 mL methanol (He et al, 2015). In addition, the reported methods used external standard method (ESM) for determination and two reference compounds were required.…”

“…The maximum absorption wavelength of analyte was usually selected as the detection wavelength because of the good sensitivity. For example, the maximum absorption wavelength 294nm was always selected as detection wavelength for HPLC analysis of honokiol and magnolol (Ma et al, 2022;Zhang et al, 2014;He et al, 2015). The UV responses of honokiol and magnolol at 294 nm were different, so the quanti cation of honokiol and magnolol needed two reference compounds.…”

Semi-Synthesis and In Vitro Anti-Cancer Evaluation of Magnolol Derivatives

“…Natural products can have many advantages over chemotherapy, such as low toxicity, low side effects, multiple targets, and reversal of resistance to cancer drugs. Magnolol (MAG, 5,5′-diallyl-2,2′-dihydroxybiphenyl), a lignan isolated mainly from Magnolia officinalis , Rehder & E.H. Wilson (“Hou Po” in Chinese) [ 8 ], exhibits a huge range of biological activities such as anti-inflammatory [ 9 ], neuroprotective [ 10 ], antibacterial [ 11 ], antioxidant [ 12 ], and antitumor [ 13 ] effects. In addition, many recent studies have shown that MAG can inhibit the proliferation, migration, metastasis, and angiogenesis of various cancer cell lines [ 14 , 15 , 16 , 17 ], suggesting that MAG has potential as a lead compound for the discovery of anticancer agents.…”