Simultaneous determination of geniposide, baicalin, cholic acid and hyodeoxycholic acid in rat serum for the pharmacokinetic investigations by high performance liquid chromatography–tandem mass spectrometry

Ran, Xiaorong; Liang, Qionglin; Luo, Guangsheng; Liu, Qingfei; Pan, Yanshu; Bing, Wang; Pang, Chunhong

doi:10.1016/j.jchromb.2006.05.001

Cited by 28 publications

(19 citation statements)

References 8 publications

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“…The relative standard deviation (RSD) was 2.2% at a concentration of 1.0 x 10 −5 g/L ( n = 11). Compared to other methods reported as shown in Table , the method proposed in the current study offered certain advantages: 1) the range of baicalin concentrations that could be detected was greater than that offered by the method; 2) the detection limit of baicalin was lower than those offered by methods; and (c) compared to the method, this method did not require tedious pretreatment.…”

Section: Resultsmentioning

confidence: 89%

CdS nanoparticles‐ enhanced chemiluminescence and determination of baicalin in pharmaceutical preparations

2012

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CdS nanoparticles (CdS NPs) of different sizes were synthesized by the citrate reduction method. It was found that CdS NPs could enhance the chemiluminescence (CL) of the luminol-potassium ferricyanide system and baicalin could inhibit CdS NPs-enhanced luminol-potassium ferricyanide CL signals in alkaline solution. Based on this inhibition, a flow-injection CL method was established for determination of baicalin in pharmaceutical preparations and human urine samples. Under optimized conditions, the linear range for determination of baicalin was 5.0 x 10(-6) to 1.0 x 10(-3) g/L. The detection limit at a signal-to-noise ratio of 3 was 1.7 x 10(-6) g/L. CL spectra, UV-visible spectra and transmission electron microscopy (TEM) were used to investigate the CL mechanism. The method described is simple, selective and obviates the need of extensive sample pretreatment.

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Section: Resultsmentioning

confidence: 89%

CdS nanoparticles‐ enhanced chemiluminescence and determination of baicalin in pharmaceutical preparations

2012

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“…A 50 mL cerebrospinal fluid or serum was analyzed as described previously (Ran et al, 2006). An applied biosystems (Toronto, Canada) API 3000 triple-quadrupole tandem mass spectrometer, equipped with a Turbo Ionspray interface and an Agilent 1100 binary HPLC system was used for LC-MS-MS analysis of cerebrospinal fluid.…”

Section: Percentage Of Infarct Volumementioning

confidence: 99%

The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFα and IL-1β after cerebral ischemia in rats

Hua

Zhu

et al. 2009

Arch. Pharm. Res.

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Previous studies have shown that Qing Kai Ling, a traditional Chinese medicine, was able to effectively prevent the inflammation from cerebral ischemia (Chen et al., 2002). The cholalic acid and hyodeoxycholalic acid (cholalic acid mixture) was major active components in Qing Kai Ling. To study the effects of cholalic acid mixture on the damage cascade of cerebral ischemia, rat model of focal cerebral ischemia was established by permanent occlusion of left middle cerebral artery. We found that the administration of cholalic acid mixture could reduce the ischemic infarct size after 24 h of ischemia, and cholalic acid mixture could be detected in cerebrospinal fluid after 2h of administration. We also found that the concentrations of tumor necrosis factor-alpha and interlukin-1beta in rat brain were significantly lower when compared to the untreated animals after 12 h and 24 h of ischemia. The concentrations of von Willebrand factor and neuron specific enolase in the plasma were remarkably decreased in cholalic acid mixture treated animals than in the untreated ones after 12h of ischemia. Our results suggested that cholalic acid mixture is able to decrease the expression of inflammation factors including tumor necrosis factor-alpha and interlukin-1beta after focal cerebral ischemia.

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“…To confirm its chemical contents, we analyzed the TLJN by using high performance liquid chromatography, as described previously (Ran et al, 2006). The major chemical components of the 5 mg/ml TLJN used in the present study were ginsenoside Rg1 (1.25 M) and its intermediates geniposide (12.36 M) as previously reported (Hua et al, 2010).…”

Section: Tljn and Its Composition Analysismentioning

confidence: 78%

“…Recent studies demonstrated that the two major components of TLJN are ginsenoside Rg1 and geniposide. Both ginsenoside Rg1 and geniposide have neuroprotective effects in culture cell of mouse (Ran et al, 2006). Ginsenoside improves performance in a passive avoidance-learning paradigm and enhances cholinergic metabolism, significantly stimulates neurite outgrowth in the absence of Nerve growth factor.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects of TongLuoJiuNao in neurons exposed to oxygen and glucose deprivation

Hou

Sun

et al. 2012

Journal of Ethnopharmacology

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Simultaneous determination of geniposide, baicalin, cholic acid and hyodeoxycholic acid in rat serum for the pharmacokinetic investigations by high performance liquid chromatography–tandem mass spectrometry

Cited by 28 publications

References 8 publications

CdS nanoparticles‐ enhanced chemiluminescence and determination of baicalin in pharmaceutical preparations

CdS nanoparticles‐ enhanced chemiluminescence and determination of baicalin in pharmaceutical preparations

The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFα and IL-1β after cerebral ischemia in rats

Neuroprotective effects of TongLuoJiuNao in neurons exposed to oxygen and glucose deprivation

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