Neuroprotective effects of TongLuoJiuNao in neurons exposed to oxygen and glucose deprivation

Li, Xiaojing; Hou, Jincai; Sun, Ping; Li, Pengtao; He, Rong; Liu, Ying; Zhao, Liyun; Hua, Qian

doi:10.1016/j.jep.2012.03.042

Cited by 27 publications

(20 citation statements)

References 31 publications

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“…A lot of studies have reported that some TCM preparations including Qing-Kai-Ling injection, An-Gong-Niu-Huang pill, Tong-Xin-Luo capsule, Nao-Shuan-Tong capsule, and Tong-Luo-Jiu-Nao capsule have been widely used in clinical practice to treat cerebrovascular diseases and showed effectiveness for the treatment of stroke by antioxidation, anti-inflammation, and neuroprotective function against ischemic reperfusion injury [14–19]. These preparations were composed of more than ten Chinese herbal medicines with a large number of complex components.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Effect of a Formula, Moschus Combined with Borneolum Synthcticum, from Traditional Chinese Medicine on Ischemia Stroke in Rats

Xia

Liu

2014

Evidence-Based Complementary and Alternative Medicine

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Moschus compatible with borneolum synthcticum is a well-known herb pair in Traditional Chinese Medicine and the present study aims to assess the neuroprotective effect of a formula composed of this herb pair on ischemia stroke in rats. The middle cerebral artery occlusion model of focal cerebral ischemia in rat was performed by using intraluminal suture method. The behavioral scores, infarct volume, and neuron ultrastructure of model and formula-treated rats were investigated after the 2 h of ischemia and 24 h of reperfusion. Meanwhile the expression levels of caspase-3, caspase-9, Bcl-2, and Bax were measured by western blot analysis. The formula treatment showed obvious neuroprotective effect according to significant decrease of the neurological scores (P < 0.01) and the infarct volumes (P < 0.05) when compared to the MCAO group. We also observed that this formula had antiapoptosis activity on neuron cell under electron microscope. Furthermore, our result supported the idea that pro- and postadministration of this formula had an antiapoptosis effect by decreasing remarkably the expression of caspase-3 and caspase-9 (P < 0.05) as well as increasing significantly the ratio of Bcl-2 to Bax (P < 0.01). All evidences demonstrated the neuroprotective effect of this formula on ischemia stroke due to decrease of brain infract volume and modulation of the expression of apoptosis-related proteins.

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Section: Introductionmentioning

confidence: 99%

Neuroprotective Effect of a Formula, Moschus Combined with Borneolum Synthcticum, from Traditional Chinese Medicine on Ischemia Stroke in Rats

Xia

Liu

2014

Evidence-Based Complementary and Alternative Medicine

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“…In previous studies, we identified that TLJN has neuroprotective effects on ischemia/reperfusion-injured neurons and brain microvascular endothelial cells [14,18]. In this study, we investigated whether TLJN had a neuroprotective effect on formaldehyde-injured cells.…”

Section: Resultsmentioning

confidence: 97%

The protective effect of geniposide on human neuroblastoma cells in the presence of formaldehyde

Sun

Chen

et al. 2013

BMC Complement Altern Med

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BackgroundFormaldehyde can induce misfolding and aggregation of Tau protein and β amyloid protein, which are characteristic pathological features of Alzheimer’s disease (AD). An increase in endogenous formaldehyde concentration in the brain is closely related to dementia in aging people. Therefore, the discovery of effective drugs to counteract the adverse impact of formaldehyde on neuronal cells is beneficial for the development of appropriate treatments for age-associated cognitive decline.MethodsIn this study, we assessed the neuroprotective properties of TongLuoJiuNao (TLJN), a traditional Chinese medicine preparation, against formaldehyde stress in human neuroblastoma cells (SH-SY5Y cell line). The effect of TLJN and its main ingredients (geniposide and ginsenoside Rg1) on cell viability, apoptosis, intracellular antioxidant activity and the expression of apoptotic-related genes in the presence of formaldehyde were monitored.ResultsCell counting studies showed that in the presence of TLJN, the viability of formaldehyde-treated SH-SY5Y cells significantly recovered. Laser scanning confocal microscopy revealed that the morphology of formaldehyde-injured cells was rescued by TLJN and geniposide, an effective ingredient of TLJN. Moreover, the inhibitory effect of geniposide on formaldehyde-induced apoptosis was dose-dependent. The activity of intracellular antioxidants (superoxide dismutase and glutathione peroxidase) increased, as did mRNA and protein levels of the antiapoptotic gene Bcl-2 after the addition of geniposide. In contrast, the expression of the apoptotic-related gene - P53, apoptotic executer - caspase 3 and apoptotic initiator - caspase 9 were downregulated after geniposide treatment.ConclusionsOur results indicate that geniposide can protect SH-SY5Y cells against formaldehyde stress through modulating the expression of Bcl-2, P53, caspase 3 and caspase 9, and by increasing the activity of intracellular superoxide dismutase and glutathione peroxidase.

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“…In recent years, the active components in TLJN were identified and widely applied in clinic and experiments [1], [2], [31]. In brief, the components are extracted from Panax notoginseng and Gardenia jasminoides .…”

Section: Methodsmentioning

confidence: 99%

“…TongLuoJiuNao (TLJN) injection liquid is an herbal medicine which is primarily composed of two active components: Ginsenoside Rg1 and Geniposide [1], [2]. Nowadays, TLJN has been used in the treatment of patients with cerebral ischemic stroke and vascular dementia [3], [4].…”

Section: Introductionmentioning

confidence: 99%

Chronic Administration of Anti-Stroke Herbal Medicine TongLuoJiuNao Reduces Amyloidogenic Processing of Amyloid Precursor Protein in a Mouse Model of Alzheimer’s Disease

Hua

et al. 2013

PLoS ONE

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Composed of Ginsenoside Rg1 and Geniposide, the herbal medicine TongLuoJiuNao (TLJN) injection liquid has anti-inflammatory properties and can improve learning and memory in mice. Recently, TLJN has been used to treat the patients with cerebral ischemic stroke and vascular dementia, which significantly increase the risk of developing Alzheimer’s disease (AD) in the early human beings. Although beneficial effects of TLJN have been reported in the vascular-associated brain disorders, the roles of TLJN in AD brains are still not clear. In this study, we chronically administered TLJN in amyloid precursor protein (APP) Swedish mutant transgenic mice (APP23) from 6 months old of age, which is at the onset of Aβ plaques, to 12 months old. We found that TLJN significantly decreased Aβ production and deposition in the brain of APP23 mice. Furthermore, we observed that TLJN down-regulated the levels and activity of β-secretase 1 (BACE1) protein as well as the expression levels of γ-secretase complex components: PS1, nicastrin and anterior pharynx-defective 1 (APH1) but not presenilin enhancer 2 (PEN2). The results suggest an inhibitory effect of TLJN on amyloidogenic APP processing by down-regulating the cleavage enzymes BACE1 and γ-secretase.

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Neuroprotective effects of TongLuoJiuNao in neurons exposed to oxygen and glucose deprivation

Cited by 27 publications

References 31 publications

Neuroprotective Effect of a Formula, Moschus Combined with Borneolum Synthcticum, from Traditional Chinese Medicine on Ischemia Stroke in Rats

Neuroprotective Effect of a Formula, Moschus Combined with Borneolum Synthcticum, from Traditional Chinese Medicine on Ischemia Stroke in Rats

The protective effect of geniposide on human neuroblastoma cells in the presence of formaldehyde

Chronic Administration of Anti-Stroke Herbal Medicine TongLuoJiuNao Reduces Amyloidogenic Processing of Amyloid Precursor Protein in a Mouse Model of Alzheimer’s Disease

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