Simultaneous determination of free ergosterol and ergosteryl esters in Cordyceps sinensis by HPLC

Yuan, Jianping; Wang, Jiang-Hai; Liu, Xin; Kuang, Hui-Cong; Zhao, Shuyan

doi:10.1016/j.foodchem.2007.04.070

Cited by 75 publications

(50 citation statements)

References 24 publications

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“…A suitable resolution might also be obtained with high performance liquid chromatography (HPLC), which is faster than GLC analysis and operates under milder column temperatures and non-destructive detection conditions (Bada et al 2004;Manzi et al 1996). (Mattila et al 2002;Teichmann et al 2007), Lactarius trivialis (Mattila et al 2002), Cordyceps sinensis (Yuan et al 2007) and other Basidiomycetes (Senatore 1992) are amongst the wild mushroom species previously studied for their contents in ergosterol. Regarding cultivated species, similar studies were conducted using Pleurotus ostreatus, Agaricus bisporus/brown, A. bisporus/white, Lentinus edodes (Mattila et al 2002;Teichmann et al 2007), Craterellus tubaeformis (Teichmann et al 2007), Tuber melanosporum, T. aestivum and T. indicum (Villares et al 2012) mushrooms.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Novel Methodology for the Analysis of Ergosterol in Mushrooms

2013

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Sterols are important molecules in the unsaponifiable fraction of several matrices. Ergosterol, which is an important vitamin D 2 precursor, is clearly the main sterol in mushrooms. Herein, an analytical method for ergosterol determination in cultivated and wild mushrooms was developed using high performance liquid chromatography coupled to ultraviolet detection. The chromatographic separation was achieved in a Inertsil 100A ODS-3 reverse phase column using an isocratic elution with acetonitrile:methanol (70:30, v:v) at a flow rate of 1 mL/min. Different extraction methodologies were tested, using n-hexane, methanol:dichloromethane (75:25, v:v) or chloroform:methanol (20:10, v:v). The method was optimized using Fistulina hepatica and proved to be reproductive and accurate. Ergosterol was the most abundant sterol by a greater extent in all mushrooms. In general, the cultivated species showed higher contents, mainly Agaricus bisporus and Lentinus edodes. Among wild species, Boletus edulis was the mushroom with the highest content in ergosterol. Results were expressed in fat content, dry weight and fresh weight bases. The assessment of ergosterol amounts might be very useful due to the bioactive potential that has been attributed to this molecule and its derivatives.

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Section: Introductionmentioning

confidence: 99%

“…The applied methodology followed a previously established procedure (Yuan et al 2007) with minor changes. Dried mushroom powder (~2 g) was extracted with methanol/dichloromethane (75:25, v/v) at room temperature in a 1:25 solid to liquid ratio.…”

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confidence: 99%

Development of a Novel Methodology for the Analysis of Ergosterol in Mushrooms

2013

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“…[7][8][9] Because the ester-type pesticides are commonly hydrolyzed in an aqueous environment, the choice of a method for its pre-concentration through adsorption prior to the HPLC-oriented analysis is limited. [10][11][12][13][14][15] The derivation of humic acid (HA) is from the microbial degradation of dead plant matter and can be identified in soil nearly everywhere with variable contents. 16 Because it is a complex mixture of numerous acids containing various components (structurally), such as quinone, phenol, catechol and sugar moieties, numerous agricultural benefits and applications exist.…”

Section: Introductionmentioning

confidence: 99%

Adsorption/Desorption of Pesticidal Carboxylate Esters by Switching Liquid Phases on Humic‐Fraction‐Modified Silica Gel Matrix

Chen

2014

J Chinese Chemical Soc

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A humic-fraction-modified silica gel is prepared and used as the adsorbent for various pesticidal carboxylate esters in hexane. The percentage of adsorption, calculated on the basis of the difference in peak areas for most carboxylate esters in hexane, reaches more than 90% in 1 h. The FTIR (Fourier Transform Infrared) Spectroscopy results show that the interaction force leading to the adsorption observed in hexane is dipole-dipole oriented and is affected negatively both by the steric hindrance created due to the presence of the group near the carbonyl center of esters and the bulky alkyl group attached to an ether linkage. The hydrogen bonding (i.e. strong dipole-dipole interaction) and p-p stacking complexation are either absent or the minor force responsible for the adsorption of carboxylate esters in hexane in most cases. Other factors that cause the variation in the percentage of adsorption include the type of liquid phase (e.g. ethyl ether or acetonitrile vs. hexane) and the additive of acidic or basic origin present in the matrix, which renders the desorption approach for analyte adsorbed.

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“…A number of methods have been reported for the quantification of ergosterol in raw materials (Polyporus umbellatus, Brassica napus, Cordyceps sinensis), such as HPLC-UV [1,[6][7][8][9][10][11], HPLC-MS/MS [12] and GC-MS [13]. Current literature shows limited information describing the quantification of ergosterol in biological samples for rat or human plasma, urine and faeces.…”

Section: Introductionmentioning

confidence: 99%