Simultaneous determination of four bufadienolides in human liver by high‐performance liquid chromatography

Abstract

A high-performance liquid chromatographic method for the simultaneous quantitation of four bufadienolides-cinobufotalin, bufalin, cinobufagin and resibufogenin-in human liver was investigated. The procedure involved solid phase extraction of human liver with an Oasis HLB cartridge coupled with reversed-phase HPLC and photodiode array detection. Recoveries obtained from spiked liver for the bufadienolides were better than 70%. The linearity was studied up to 1.2 mg/kg and the detection limits of the method were 0.4 ng for cinobufotalin and bufalin and 0.5 ng for cinobufagin and resibufogenin. The developed method was successfully applied to a lethal poisoning case.

Traditional Chinese medicine in the prevention and treatment of cancer and cancer metastasis

“…Chan Su is another single herb with anticancer activity. Bufadienolides, including bufalin, cinobufagin, resibufogenin and telocinobufagin, are the major active constituents derived from Chan Su (32). Chan Su and its constituents have been reported to be able to suppress cellular growth and promote apoptosis in leukaemia, liver, lung and prostate cancers (19).…”

Apoptosis-inducing activity of compounds screened and characterized from cinobufacini by bioassay-guided isolation

“…These two active compounds were identified as resibufogenin and cinobufagin, which are members of the bufadienolide family. Bufadienolides, including resibufogenin, cinobufagin, bufalin, bufotalin and arenobufagin, are a type of steroidal cardiac glycosides (17) that exhibit a variety of biological effects, including cardiotonic, anesthetic and antineoplastic activities (18). Bufalin, as a major active compound of bufadienolide, has been demonstrated to possess significant anti-proliferative activities and apoptosis-inducing effects on human leukaemia, liver, prostatic, endometrial and ovarian cancer cells (19,20).…”

Study of the determination and pharmacokinetics of bufadienolides in dog's plasma after administration of Liu-Shen-Wan by high performance liquid chromatography time-of-flight mass spectrometry

“…Recent literature revealed that several methods have been used to determine bufadienolides in toad venom [8][9][10], in traditional Chinese medicines formula [11][12][13] and in human liver [14]. In all of these reports, reversedphase HPLC/UV was used as the quantitative determination technique.…”