Simultaneous determination of flavonoids and phenylethanoids in the flowers of <i>Verbascum densiflorum</i> and <i>V. phlomoides</i> by high‐performance liquid chromatography

Klimek, Barbara; Olszewska, Monika A.; Tokar, Magdalena

doi:10.1002/pca.1171

Cited by 28 publications

(22 citation statements)

References 16 publications

(31 reference statements)

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“…Among them, four compounds were unequivocally identified as plantamajoside (10), isoplantamajoside (19), acteoside (16), and isoacteoside (22) by comparing their retention times, MS, and MS/MS data with those of reference compounds. Nine compounds, meanwhile, were tentatively identified as hellicoside (2), 2‐hydroxyacteoside (6), 2‐acetylacteoside (9), forsythiaside (12), campneoside (13), crenatoside (15), calceorioside B (18), leucosceptoside A (23), and forsythoside B (25) by comparing their MS and MS/MS data with those reported in the literatures []. For example, the compound of m / z 655.3 at 1.99 min produced amount of fragments at m / z 639.2, 493.1, 341.1, 255.3, 179.0, and 161.1.…”

Section: Resultsmentioning

confidence: 99%

A novel strategy for target profiling analysis of bioactive phenylethanoid glycosides in Plantago medicinal plants using ultra‐performance liquid chromatography coupled with tandem quadrupole mass spectrometry

Xiong

Geng

et al. 2012

J of Separation Science

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Phenylethanoid glycosides are a group of phenolic compounds with diverse biological activities such as hypotensive, diuretic, and hypoglycemic effects. In this study, a target profiling analysis approach using ultra-performance liquid chromatography coupled with tandem quadrupole mass spectrometry (MS) was established on the basis of parent ion scanning for m/z 161, the characteristic product ion for phenylethanoid glycosides. It was successfully employed to discriminate the chemical composition of phenylethanoid glycosides between Plantaginis Herba and Plantaginis Semen, two medicinal parts of Plantago plants, which are widely used as herbal medicine in China. Totally, 34 phenylethanoid glycosides were characterized and tentatively identified by their retention times, MS, and tandem quadrupole MS (MS/MS) data. Combined with chemometrics analysis of principal component analysis and orthogonal projection to latent structural discriminate analysis, eight of them, especially acteoside and plantamajoside, were picked out and contributed to the chemical distinction between Plantaginis Herba and Plantaginis Semen, which might be responsible for the differences in diuretic and hypotensive effects between the two medicinal parts. This new approach for target profiling provides not only a novel idea for specific analysis of active chemical constituents in the same type, but also a promising and reference method for quality evaluation of traditional Chinese medicines.

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Section: Resultsmentioning

confidence: 99%

A novel strategy for target profiling analysis of bioactive phenylethanoid glycosides in Plantago medicinal plants using ultra‐performance liquid chromatography coupled with tandem quadrupole mass spectrometry

Xiong

Geng

et al. 2012

J of Separation Science

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“…Mullein flowers are highly valued herbal drugs used in the treatment of inflammation, asthma, spasmodic coughs and other respiratory tract diseases (Klimek et al, 2010). For some Verbascum species, the anti-inflammatory effect has been confirmed: V. pycnostachyium Boiss.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have reported that aucubin (iridoid) and verbascoside (acteoside) (phenylethanoid) are the major compounds responsible for anti-inflammatory activity (Recio et al, 1994;Schapoval et al, 1998). However, there are some assumptions that claim the anti-inflammatory and antimicrobial activities of the herb are directly correlated to the polyphenol content (Klimek et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Grigore

Colceru-Mihul

Liţescu

et al. 2013

Pharmaceutical Biology

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Context: Verbascum phlomoides L. (Scrophulariaceae) (mullein) used in the European folk medicine due to its anti-inflammatory and soothing action on the respiratory tract is thoroughly documented in handbooks and scientific literature. Nevertheless, information regarding the influence of the polyphenol content on pharmacological activity is scarce. Objective: This study explored the antioxidant and anti-inflammatory potential of V. phlomoides polyphenol-rich extract. Materials and methods: Dried mullein flowers (200 g) were subjected to water extraction (60 C, 2 h, herb/solvent ratio ¼ 1/10 m/v) and further to n-butanol partition. Total phenolics were spectrophotometrically determined and specific compounds were evaluated by HPLC. The antioxidant activity was assessed by the 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) assay. The anti-inflammatory potential of the extract (50-200 mg/mL) was evaluated in vitro by ELISA measurement of ICAM-1 expression in TNF-a-stimulated endothelial cells and in vivo by the rat paw edema assay. Results: The mullein extract contained 4.18% total polyphenols expressed as gallic acid. The main components identified by HPLC were: rosmarinic acid (14.93 mg/g), caffeic acid (39.96 mg/g), ferulic acid (29.61 mg/g) and quercetin (17.29 mg/g). Acteoside was not detected; aucubin was detected in traces (0.028 mg/g). Depending on concentration, the extract exerted scavenging activity on DPPH radical (EC 50 7.09 mg/mL), significantly inhibited TNF-a-induced ICAM-1 expression by 55-58.8% on human umbilical vein endothelial cells at 100 and 200 mg/mL, but failed to reduce egg-white-induced rat paw edema. Discussion and conclusion: Mullein polyphenols play an important role in exerting the antioxidant effect but have a weak influence on anti-inflammatory activity that is correlated, probably, to a higher content of iridoids and phenylethanoids.

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“…Chromatographic separation was achieved using a SHISEIDO column (C 18 5 µm, 4.6 I.D. × 250 mm) and the column temperature was kept at 35 o C. The mobile phase was consisted of water (A) containing 0.1% TFA and MeOH (B) with gradient elution to improve the separation capacity (Klimek et al, 2010). The gradient elution system was optimized and performed as follows; 10-30% B (0-20 min), 30-50% B (20-45 min), 50-70% B (45-55 min) and 70-75% B (55-65 min) at a flow rate of 1.0 mL/min.…”

Section: The Hplc Conditionsmentioning

confidence: 99%

Simultaneous determination of eight marker compounds in the traditional herbal medicine, sipjundaebo-tang by HPLC-DAD

et al. 2011

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Sipjundaebo-tang, known as a traditional herbal medicine, has been used in the treatment of anemia, inflammation, and tumor. For simultaneous determination of eight components, namely 5-HMF, paeoniflorin, ferulic acid, cinnamaldehyde, decursinol, 6-gingerol, decursin, and glycyrrhizin in Sipjundaebo-tang, a high performance liquid chromatography-diode array detector (HPLC-DAD) method was established. In order to develop and validate this HPLC-DAD method, C(18) column (S-5 μm, 4.6 × 250 mm) was used with gradient mobile phase at the column temperature of 35°C. The mobile phase was consisted of water and methanol containing 0.1% trifluoroacetic acid. UV wavelength was set at 230, 254, 280, and 300 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test. Calibration curve for eight marker compounds showed good linearity with R(2) > 0.9994. Limits of detection and Limits of quantification ranged from 0.01 μg/mL to 0.13 μg/mL and 0.03 μg/mL to 0.41 μg/mL, respectively. The relative standard deviations (RSDs) value of precision test, intra-day and inter-day test, was less than 1.14% and 2.54%, respectively. The results of accuracy test were varied from 98.31% to 104.88% with RSD < 2%. This developed simultaneous determination method was efficient to the quality control of Sipjundaebo-tang.

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Simultaneous determination of flavonoids and phenylethanoids in the flowers of Verbascum densiflorum and V. phlomoides by high‐performance liquid chromatography

Abstract: The HPLC method established is appropriate for the quality assurance and the differentiation of V. phlomoides and V. densiflorum samples.

Cited by 28 publications

References 16 publications

A novel strategy for target profiling analysis of bioactive phenylethanoid glycosides in Plantago medicinal plants using ultra‐performance liquid chromatography coupled with tandem quadrupole mass spectrometry

A novel strategy for target profiling analysis of bioactive phenylethanoid glycosides in Plantago medicinal plants using ultra‐performance liquid chromatography coupled with tandem quadrupole mass spectrometry

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Simultaneous determination of eight marker compounds in the traditional herbal medicine, sipjundaebo-tang by HPLC-DAD

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