Simultaneous determination of five toxic alkaloids in body fluids by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Qiu, Peihong; Chen, Xiaohong; Chen, Xiang; Lin, Li; Ai, Changchun

doi:10.1016/j.jchromb.2008.09.034

Cited by 31 publications

(21 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9 Some of these derivatives have been characterized by means of mass spectra methods, but the mass spectrometry of 10-alkylthiocolchcines has not been published so far, while the EI and ESI mass spectra of colchicine itself are known and described in chemical literature. 10,11 The MS methods have been used to establish fragmentation patterns of some colchicine analogues and for detection and identification of components in Colchicum species, whereas mass spectrometry with liquid chromatography, LC/ESI-MS/MS or LC-APCI-MS, have found application in detection of colchicine in sheep serum and milk and in human blood plasma. 10,[12][13][14][15][16][17][18] In the present work five 10-alkylthiocolchicine derivatives ( Figure 1) were investigated for the principal fragmentation pathways of the molecular ions using EI and protonated molecules using MALDI mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometric study of colchicine and its synthetic derivatives 10-alkylthiocolchicines

Kurek

Nowakowska

Bartkowiak

2015

ACSi

View full text Add to dashboard Cite

The mass spectra behaviour of five 10-alkylthiocolchicine derivatives has been studied using electron ionization and MALDI-TOF MS techniques. Fragmentation patterns of colchicines derivatives after EI have been investigated, as well as mass spectra after collision-induced dissociation (CID) MALDI have been used to gain structural information. To the best of our knowledge, this is the first time that the MALDI MS/MS data for colchicine and its alkylthio derivatives have been described. It has been shown also that the data derived from mass spectra can be used for identification/quantitative determination of natural and modified alkaloids of colchicine group. The detailed fragmentation pathways proposed here could be helpful for the characterization of other colchicines of these type. The utility of different ionization techniques for analysis of compounds of this class has been evaluated. Due to the cytotoxic activity towards tumour cell lines, 10-alkylthiocolchicines may be considered as the active ingredients of anticancer agents. If these compounds find use in medical treatment, their distribution in organism and their metabolism will have to be monitored by spectroscopic or spectrometric methods. The characteristic fragment ions may be used by Selected Reaction Monitoring method for determination of colchicine analogues.

show abstract

Section: Introductionmentioning

confidence: 99%

Mass spectrometric study of colchicine and its synthetic derivatives 10-alkylthiocolchicines

Kurek

Nowakowska

Bartkowiak

2015

ACSi

View full text Add to dashboard Cite

show abstract

“…Some analytical methods have been developed for the determination of alkaloids in biological samples, such as gas chromatography (GC) [10], high-performance liquid chromatography (HPLC) [11], capillary electrophoresis (CE) [12,13], gas chromatography coupled with mass spectrometry (GC-MS) [14][15][16], and high-performance liquid chromatography coupled with (tandem) mass spectrometry (LC-MS, LC-MS/MS) [17][18][19][20][21][22][23]. In spite of the good separation capacity of GC or GC-MS, these two instruments were limited in analysis of most alkaloids of low volatility.…”

mentioning

confidence: 99%

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

Zhang

Huang

et al. 2012

Chromatographia

View full text Add to dashboard Cite

A sensitive and selective method for simultaneous determination of 29 toxic alkaloids in human blood and 31 in urine using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was developed and validated. The samples were diluted with 0.1 mol L -1 HCl, and the target alkaloids were purified by solid phase extraction. The separation of 31 alkaloids was carried out on a C18 column using a gradient mobile phase with 10 mmol L -1 ammonium formate in water with 0.1% formic acid and methanol at the rate of 0.25 mL min -1 . The triple-quadrupole mass spectrometer equipped with an electrospray source in the positive mode was set up in the dynamic multiple reactions monitoring mode (dynamic MRM) to detect the ion transitions of 31 alkaloids. The calibration curves were linear over a range of 0.5-400, 1-400, or 4-400 lg L -1 for target alkaloids in human blood and urine, and the correlation coefficients (r 2 ) was higher than 0.9943. The limit of determination and limit of quantification were 0.2-1 and 0.5-4 lg L -1 for blood and urine, respectively. The only exceptions were sanguinarine and chelerythrine in human blood. All the target alkaloids were stable under the test condition. In addition, the solvent effect and reconstituted solution were investigated. The method was validated and proved to be accurate and precise over the studied concentrations and suitable for poisoning diagnosis and forensic toxicology.

show abstract

“…Some studies reported several MS methods to detect aim compounds. 25,26 So the current study sought an easy applicable alternative (PDA) to the sophisticated HPLC-tandem MS or GC-MS method in view of the limited financial resources of many laboratories. Table 3 describes the methods used to determine strychnine in several matrices in recent years.…”

Section: Comparison Of Published Methods To Determine the Analytesmentioning

confidence: 99%

Fabrication of Fe3O4 Nanoparticle-coalesced Hydroxylated Multiwalled Carbon Nanotubes for the Analysis of Strychnine in Human Serum

Feng

Wei

et al. 2015

ANAL. SCI.

View full text Add to dashboard Cite

Simultaneous determination of five toxic alkaloids in body fluids by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Cited by 31 publications

References 21 publications

Mass spectrometric study of colchicine and its synthetic derivatives 10-alkylthiocolchicines

Mass spectrometric study of colchicine and its synthetic derivatives 10-alkylthiocolchicines

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

Fabrication of Fe3O4 Nanoparticle-coalesced Hydroxylated Multiwalled Carbon Nanotubes for the Analysis of Strychnine in Human Serum

Contact Info

Product

Resources

About