Simultaneous Determination of Five Antioxidants in Food by HPLC with Fluorescence Detection.

Abstract

An HPLC method with fluorescence detection was developed for the determination of propyl gallate, nordihydroguaiaretic acid, butylated hydroxyanisole (2-and 3-tert-butyl-4-hydroxyanisole), tert-butylhydroquinone and octyl gallate in edible oils and foods.The antioxidants in edible oil were isolated directly with acetonitrile saturated with n-hexane. The antioxidants in food were extracted with ethyl acetate and the extract was concentrated under vacuum. They were isolated from the residue with acetonitrile saturated with n-hexane. The acetonitrile layer was centrifuged at 5,000 rpm for 10 min. The HPLC separation was performed on a Symmetry C18 column (3.5 mm, 4.6 mm i.d.150 mm) using a mixture of 5 acetic acidῌacetonitrileῌmethanol (4 : 3 : 3, v/v/v) as the mobile phase and monitored by using a fluorescence detector with time programming. Sample peaks were identified by comparison of the fluorescence spectra with those of antioxidant standards. Average recoveries of fortified antioxidants at 100 mg/g were 72.1ῑ99.6. Coe$cients of variation were 0.7ῑ7.2.

Methods for Total Antioxidant Activity Determination: A Review

“…Average recoveries of fortified antioxidants at 100 micrograms/g were 72.1-99.6%. Coefficients of variation were 0.7-7.2% [118].…”

“…A HPLC method with fluorescence detection [118] was developed for the determination of propyl gallate, nordihydroguaiaretic acid, butylated hydroxyanisole, tert-butylhydroquinone and octyl gallate in edible oils and foods. The HPLC separation was performed on a C18 column using a mixture of 5% acetic acid-acetonitrile-methanol as the mobile phase and monitored by using a fluorescence detector.…”

Validação de metodologia para a determinação simultânea dos antioxidantes sintéticos em óleos vegetais, margarinas e gorduras hidrogenadas por CLAE/UV

“…Esses métodos incluem desde a detecção qualitativa através de reações colorimétricas até métodos semiquantitativos e quantitativos, como espectrofotometria, voltametria, polarografia e métodos cromatográficos, tais como cromatografia em camada delgada -CCD, 9,10 de permeação em gel -CPG, 11 em fase gasosa -CG, 12-15 líquida de alta eficiência -CLAE [16][17][18][19][20][21][22] e eletroforese capilar -CE. 23 Dos métodos citados, CG e CLAE são os mais empregados para a separação e quantificação dos antioxidantes sintéticos.…”

“…[17][18][19][20][21][22][23]27,[32][33][34] No Brasil, quase não existem dados sobre os níveis reais de antioxidantes presentes nos alimentos. Portanto, é de fundamental importância desenvolver um método analítico que seja adequado para a determinação simultânea dos antioxidantes sintéticos permitidos pela legislação brasileira.…”

Preparation of ZnO-Loaded Lignin-Based Carbon Fiber for the Electrocatalytic Oxidation of Hydroquinone

“…Developing a suitable and efficient analysis method has attracted the interest of many researchers in recent years [1]. So far, methods such as high-performance liquid chromatography (HPLC) [2], pH-based flow injection analysis [3], and synchronous fluorescence [4] have been employed for the determination of hydroquinone. However, the methods above are limited in many applications due to their high cost and complex analysis procedures.…”