Methods for Total Antioxidant Activity Determination: A Review

Pisoschi, Aurelia Magdalena; Negulescu, Gheorghe Petre

doi:10.4172/2161-1009.1000106

Cited by 309 publications

(319 citation statements)

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“…Antioxidant activity is always measured in an indirect way as a response (of the antioxidants present in the sample) to induced oxidation [192,160,173]. For foodstuffs, there is a range of methods for determining antioxidant activity.…”

Section: Methods For Measuring the Antioxidant Activity Of Anthocyaninsmentioning

confidence: 99%

“…Antioxidant activity/capacity measurement: classification, physicochemical principles, mechanisms and electron transfer-based assays [154] Antioxidant activity/capacity measurement: hydrogen atom transfer-based, mixed-mode and lipid peroxidation assays [155] Antioxidant activity/capacity measurement: reactive oxygen and nitrogen species scavenging assays, oxidative stress biomarkers and chromatographic/chemometric assays [156] Recent applications for in vitro antioxidant activity assay [157] Evaluation of procedures for assessing anti-and pro-oxidants in plant samples [158] Capacity of antioxidants to scavenge multiple reactive oxidants and to inhibit plasma lipid oxidation induced by different biological oxidants [159] Analytical methods applied to antioxidant and antioxidant capacity assessment in plant-derived products [160] Advantages and limitations of commons testing methods for antioxidants [161] A comprehensive overview on the biology behind some reactive molecules and the means for their detection [162] Potentiometric study of antioxidant activity: development and prospects [163] Methods for determining the efficacy of radical-trapping antioxidants [164] Electrochemical methods for total antioxidant capacity [165] The role of consumption of dietary bioactives on the prevention of adverse health [166] Synthetic and natural phenolic antioxidants: mode of action, health effects, degradation products and toxicology [167] Up-to-date overview of methods available for measuring antioxidant activity [168] Use of metallic nanoparticles and quantum dots as novel tools for reliable assessment of antioxidant activity in food and biological samples [169] Review on in vivo and in vitro methods evaluation of antioxidant activity [170] IUPAC technical report: methods of measurement and evaluation of natural antioxidant capacity/activity [171] Evaluating the antioxidant capacity of natural products: a review on chemical and cellular-based assays [172] Application of free radical diphenylpicrylhydrazyl to estimate the antioxidant capacity of food samples [173] Application of both stationary and flow electrochemical methods for analysis of antioxidant properties of plant and clinical samples [174] Phenol-based antioxidants and the in vitro methods used for their assessment [175] Main components in the foodstuffs and beverages: antioxidant methods, chemical and kinetic basis …”

Section: Content Referencesmentioning

confidence: 99%

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Antioxidant Capacity of Anthocyanin Pigments

Martín¹,

Kuskoski²,

Navas³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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Anthocyanins are a family of natural pigments classified into the group of flavonoids, considered to be responsible for the color and taste of many fruits and vegetables, i.e. berries. Anthocyanins are common components of the human diet. Besides their interest as colorant because of their coloring properties, the study of anthocyanin compounds stems from their wide applicability in the prevention and even in the treatment of various human diseases. However, various aspects of the pharmacological roles of anthocyanins remain in the dark, having still several obstacles to the development of robust diets or prescribing lines on consumption of anthocyanins. The chemical structure of anthocyanins determines in large measure its capacity and efficacy as an antioxidant agent. In this study, the following aspects are reviewed: the antioxidant effect of anthocyanin pigments; the oxidative stress, the bioavailability after intake and biological aspects of anthocyanins, the method for measuring the antioxidant activity of anthocyanins, the relationship between structure and activity; and the influence of the anthocyanins in the antioxidant activity of wines. Finally an overview of some potential uses in food industry is attempted mainly focusing in the anthocyanin encapsulation topic. Attention has been paid to the more recent publications in the field.

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Section: Methods For Measuring the Antioxidant Activity Of Anthocyaninsmentioning

confidence: 99%

Section: Content Referencesmentioning

confidence: 99%

Antioxidant Capacity of Anthocyanin Pigments

Martín¹,

Kuskoski²,

Navas³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

View full text Add to dashboard Cite

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“…Investigation of the antioxidant potential with particular concern of ecological and geographical distribution of plant accessions has become of interest in recent studies (Pisoschi and Negulescu, 2011). Although cumin accessions can exhibit marked variations in antioxidant content owing to their different ecogeographical distribution, clustering based on SDS-PGE and RAPD data was not identical with that based on the antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

Genetic linkage between protein and DNA polymorphisms and antioxidant capacity of Cuminum cyminum L. accessions

Abdelhaliem

Al-Huqail

2016

Genet. Mol. Res.

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ABSTRACT. This study aimed to link the genetic variation observed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD) analysis among 11 Cuminum cyminum L. accessions, collected from diverse ecogeographical areas in Saudi Arabia, with their antioxidant capacity to better identify potential genotypes for breeding programs for this medicinal spice. SDS-PAGE analysis revealed genetic variation among cumin germplasms and distinct polymorphisms (100%). Protein polymorphisms were identified based on the number of polypeptide bands (288) with molecular weights ranging from 13.85 to 350 kDa, band intensity, the appearance of new bands, and the absence of other bands. RAPD analysis revealed 363 amplified DNA products with a high polymorphism value (98.88%) based on DNA band type (unique, non-unique, and monomorphic), DNA 90 to 1085-bp long, and band intensity. The unweighted pair group method with arithmetic mean clustering based on SDS-PAGE or RAPD and Jaccard's similarity coefficient divided cumin accessions into similar but distinct clusters with respect to their location of collection. The antioxidant potential of cumin accessions based on 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity, the β-carotene-linoleate model system, and total phenolic and flavonoid contents revealed distinct variability. These data indicate that cumin is a valuable genetic resource with high antioxidant activity. Additionally, clustering based on antioxidant activity was not identical to that based on SDS-PAGE and RAPD. Data and clustering of SDS-PAGE and RAPD, combined with the high antioxidant capacity of cumin accessions, are important for the efficient use of genetic resources of cumin in breeding strategies and genetic improvement programs.

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“…These results are might be due to high phenolics and flavonoids content found in this plant. Furthermore, there are plenty of reports suggesting the use of antioxidants as free radical scavengers for most type oxidizing molecules including ROS [7,14]. For instance, the smaller value of IC 50 corresponds to a higher antioxidant activity of the plant extract [14].…”

Section: Discussionmentioning

confidence: 99%

“…In the meantime, epidemiological studies have demonstrated that naturally antioxidant in plants such as phenolic and flavonoid compounds possess a protective role against the development of pathological conditions such as hypertension and obesity which associated to oxidative stress [6]. The oxidation processes by oxidants can be delayed or inhibited by the antioxidant compounds [7].…”

mentioning

confidence: 99%

Antioxidant and Antihypertensive Effect of Azadirachta Excelsa Leaf Extract in Spontaneously Hypertensive Rat (SHR) Model

S.¹,

S.²,

Nurdiana³

et al. 2016

ACIM

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A research was carried out to evaluate the antioxidant activities of Azadirachta excelsa and its antihypertensive effect in Spontaneously Hypertensive Rat (SHR). The Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) was quantified and IC 50 level of A. excelsa was determined. For the antihypertensive effect, the rats were randomly assigned into four treatment groups as followed: Group I (normotensive control from Wistar-Kyoto rats), Group II (hypertensive control from SHR), Group III (SHR receiving 250 mg/kg of A. excelsa extract), and Group IV (SHR receiving 40 mg/kg of captopril). The Systolic Blood Pressure (SBP) of these animals was performed by tail-cuff method. The average of TPC and TFC was 202 ± 0.42 mg Gallic Acid Equivalent (GAE)/g extract and 198 ± 0.67 mg rutin equivalent/g extract, respectively. Meanwhile, the IC 50 value of free radical scavenging activity was about 308 μg/ml. The systolic blood pressure level of the SHR treated with A.excelsa significantly reduced (153 mmHg; P <0.05) compared to the untreated SHR control (187 mm Hg; Group II). In conclusion, we found that A. excelsa extract at a dose of 250 mg/kg possesses phenolic properties that can be used as a potential treatment for hypertension due to its high antioxidant activities.

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Methods for Total Antioxidant Activity Determination: A Review

Cited by 309 publications

References 75 publications

Antioxidant Capacity of Anthocyanin Pigments

Antioxidant Capacity of Anthocyanin Pigments

Genetic linkage between protein and DNA polymorphisms and antioxidant capacity of Cuminum cyminum L. accessions

Antioxidant and Antihypertensive Effect of Azadirachta Excelsa Leaf Extract in Spontaneously Hypertensive Rat (SHR) Model

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