Simultaneous Determination of Ferulic Acid and Phthalides of Angelica Sinensis Based on UPLC-Q-TOF/MS

Abstract

Abstract:The radix of Angelica sinensis (AS) is one of the most commonly used as a herbal medicine. To investigate the geoherbalism and quality evaluation of AS, an ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established to analyze and identify ferulic acid and phthalides in AS. The results showed that among samples collected in four regions, the relative contents of ferulic acid and phthalides were highest in samples collected in Gansu, and the samples from the four different regions were apparently classified into four groups. Meanwhile, the relative content in non-fumigated root was higher than after sulfur-fumigation and the sulfur-fumigated and non-fumigated samples were obviously divided into two groups by PCA. The paper establishes a systematic and objective evaluation system to provide a scientific basis for evaluating the quality of AS.

Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats

“…Diels has been considered to be “female’s ginseng” mainly because of its excellent therapeutic effect for gynecological diseases, such as dysmenorrhea, irregular menstruation, premenstrual, and menopausal symptoms ( Huntley and Ernst, 2003 ; Haines et al, 2008 ; Hook, 2014 ). Ferulic acid, ligustilide and angelica polysaccharide are considered to be the main bioactive substances of Angelica ( Wei and Huang 2015 ; Yang et al, 2019 ) Studies have confirmed that Angelica sinensis (Oliv.) Diels extract can be developed for the treatment of endometriosis by inhibiting inflammatory reactions ( Xiong et al, 2020 ) and could significantly decrease NF-kB/β-actin and IL-6/β-actin mRNA expression in the uterus of rats with pelvic inflammation ( Huang et al, 2012 ).…”

Simultaneous determination of eight components in Angelica sinensis based on UHPLC‐ESI‐MS/MS method for quality evaluation

“…Furthermore, its ability to monitor the productions of precursor and ion can increase specificity in detection and identification of known compounds (Li et al, ). Wei et al ., (Wei & Huang, ) developed UHPLC‐Quadrupole time of flight‐MS (UHPLC‐QTOF‐MS) method, which was then applied to simultaneously identify ferulic acid and phthalides from crude and processed A. sinensis samples from four different regions. Despite these studies, the identification and determination of multiple components in A. sinensis using LC‐ESI‐MS/MS have not been reported, and the qualities of A. sinensis from different regions have not been compared.…”

Screening active components of modified Xiaoyao powder as NRF2 agonists

“…The monomer compound standards (purity >98%) were purchased according to the results of related researchs on herbs containing in MXP, which was purchased from Taiwan Xian Feng Department of Medicine (No. 034057): chlorogenic acid, salvianolic acid A, gallic acid, kaempferitrin, gardenoside, paeoniflorin, glycyrrhetinic acid, isorhamnetin, isoliquiritin, β‐sitosterol, pachymic acid, ursolic acid, isoquercitrin, quercetin (Figure A), kaempferol (Figure B), atractylenolide‐II (Figure C), rutin, liquiritin, paeonol, crocin, saikosaponin A, saikosaponin B2, saikosaponin C, saikosaponin D, and ferulic acid.…”

“…The monomer compound standards (purity >98%) were purchased according to the results of related researchs [11][12][13][14][15][16][17][18] Figure 1A), kaempferol ( Figure 1B), atractylenolide-II ( Figure 1C), rutin, liquiritin, paeonol, crocin, saikosaponin A, saikosaponin B2, saikosaponin C, saikosaponin D, and ferulic acid. Among them, quercetin and glycyrrhetinic acid were purchased from National Institutes for Food and Drug Control, and the rest of the standards, also including curcumin, were purchased from Munster Biological Technology Co Ltd (Chengdu, China).…”