Simultaneous Determination of Ethinylestradiol and Levonorgestrel in Oral Contraceptives by Derivative Spectrophotometry

Abstract

A method for determining ethinylestradiol (ETE) and levonorgestrel (LEV) in mixtures by first-derivative spectrophotometry is described. The procedure does not require any separation step. Measurements are made at the zero-crossing wavelengths and the calibration graphs are linear up to 26 and 33 micrograms ml-1 of ETE and LEV, respectively. The method was applied to the determination of both compounds in five different Spanish commercial low-dose oral contraceptives. Similar results were obtained by an HPLC method.

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…An example for this is the determination of triamcinolone acetonide in an ointment. 23 The same technique using the "zero-crossing" method was found to be suitable for the simultaneous determination of the components of dual-component drug formulations such as creams and tablets containing triamcinolone acetonide and terbinafine hydrochloride 24 and oral contraceptives containing ethinylestradiol and levonorgestrel 25,26 as well as ethinylestradiol and gestodene. 27 A zero-crossing second-derivative method was used for the determination of fluorometholone and tetrahydrozoline hydrochloride 28 as well as estradiol and medroxyprogesterone acetate 29 in formulations.…”

“…Partial least-squares regression analysis was applied to other diuretic preparations, such as spironolactone-althiazide 15 and spironolactone-chlorthalidone 16 formulations. The much more delicate problem of the assay of the low-dosed levonorgestrel-ethinylestradiol 17 and gestodene-ethinylestradiol 18 oral contraceptive tablets was also solved by the partial least-squares regression analysis. Even three-component drug formulations were successfully analyzed by this and other chemometric methods, such as the principal component regression method.…”

Simultaneous determination of levonorgestrel and ethinyl estradiol in combined dosage form utilizing spectrophotometric methods and high performance thin layer chromatographic method on nanosilica gel plates

“…The reported first derivative spectrophotometric methods [19,20] have shown the disadvantage that the zero crossing point of EE at λ 249 nm does not correspond to a peak maximum or valley for LEV which affects the accuracy and sensitivity of the methods. While the reported H-point standard addition methods [21][22][23] had the disadvantage of several calculation steps which could be considered complicated.…”

“…This combination is official in United States Pharmacopoeia (USP), which describes a chromatographic method for its estimation [8]. In addition, RP-HPLC [9], HPLC-tandem mass spectrometry [10,11], molecular imprinted polymer-HPLC [12], immune-affinity chromatography [13], HPTLC [14][15], micellar electrokinetic chromatography (MEKC) [16], voltammetry [17], multivariate calibration technique of partial least squares (PLS) and principal component regression (PCR) [18], first derivative spectrophotometry [19,20] and H-Point standard addition spectrophotometry [21][22][23] were developed for the determination of LEV and EE in biological and pharmaceutical matrixes. It is obvious that most of the reported methods for the determination of LEV and EE have utilized hyphenated instrumentation whose use is considered a tedious process.…”

DETERMINATION AND CO-ESTIMATE OF THE CHLORMADINONE ACETATE AND 17α-ETHINYL ESTRADIOL IN PHARMACEUTICAL FORMULATION AND DRINKING WATER SAMPLES BY DIGITAL DERIVATIVE SPECTOPHOTOMETRY

“…Developing new drugs and formulations, involves the qualitative and quantitative analysis of these drugs in pharmaceutical formulations. pharmaceutical formulations has been reported by the following methods: Voltammetry 3,4 ; capillary electrophoresys 5,6 , HPLC-TMS 7 , HPLC [8][9][10] , derivative spectrophotometry [11][12][13][14][15][16] and multivariate analysis based on the spectrophotometric data 11,12 . The simultaneous determinations of EEL and CMA in pharmaceutical formulations and water samples have not been reported.…”