Simultaneous determination of erlotinib and metabolites in human urine using capillary electrophoresis

Abstract

The purpose of this study was to develop a simple and sensitive CE-UV method to quantify erlotinib and metabolites in urine. Following liquid-liquid extraction, erlotinib, and metabolites were separated with a BGE whose composition was phosphate buffer (pH 2.5, 65 mM) with 0.5% Tween 20. The applied voltage was 22 kV, capillary temperature 25°C and the sample injection was performed in the hydrodynamic mode. All the analyses were carried out in a fused silica capillary with an internal diameter of 75 μm and a total length of 37 cm. The detection of target compounds was performed at 240 nm. The calibration was linear in the range 0.15-20 mg/L for erlotinib and metabolites. Inter-and intraday imprecision were less than 4%. This simple, sensitive, accurate, and cost-effective method can be used in routine clinical practice to monitor erlotinib concentrations in urine from nonsmall cell lung cancer patients.

Simultaneous determination of erlotinib and its isomeric major metabolites in human plasma using isocratic liquid chromatography–tandem mass spectrometry and its clinical application

“…Several clinical reports have been published on the method evaluating the plasma concentration of erlotinib and its metabolites using various chromatographic techniques (Jiongwei et al ., ; Lankheet et al ., ; Lepper et al ., ; Masters et al ., ; Rodríguez et al ., ; Zhang et al ., ; Zhao et al ., ). These methods determined the concentration of OSI‐420 as a total amount of OSI‐413 and OSI‐420, since separation of these positional isomers using liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) is difficult because of their similar chemical structures and identical molecular weights.…”

An Exploration of Advancement in Analytical Methodology for Quantification of Anticancer Drugs in Biomatrices

“…57 Each person has a different pharmacokinetic rate and to understand the adsorption, distribution, metabolism, excretion and toxicity of any drug, its determination in urine along with plasma is required. 58 Advantages of urine as a matrix are the requirement of easy collection and sample preparation procedure, and less interference from matrix components leading to less complicated analysis.…”

“…The capillary was maintained at 25 C and detection was performed at 240 nm with a UV detector. 58 An icotinib quantification method was developed by Liu et al using LLE and an Xterra C18 column for separation. The mobile phase consisting of a mixture of ammonium acetate (55%) and acetonitrile (45%) and chromatographic run time was 2.5 min.…”

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

“…Basic CE‐UV has been reported as a successful approach for the detection and monitoring of several drugs. Such studies have been described for the determination of cocaine, cocaethylene, heroin, morphine, 6‐monoacetylmorphine, and 4‐methylmethcathinone in urine , cocaine and its major metabolites in urine and hair samples , and erlotinib and its metabolites also in urine . The use of chiral selectors in CE has been suggested as a viable way to analyze drugs in body fluids, tissues, and in cell‐culture fluids .…”