Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Phillips, Terry M.

doi:10.1002/elps.201700283

Cited by 33 publications

(18 citation statements)

References 138 publications

(138 reference statements)

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“…Szczotka SPME umożliwiająca jednoczesną analizę 96 próbek oznaczania związków egzo-i endogennych są techniki separacyjne, w tym techniki chromatograficzne i elektroforetyczne [35,36]. Jedną z najczęściej stosowanych metod analizy próbek biologicznych jest wysokosprawna chromatografia cieczowa (ang.…”

Section: Oznaczanie Leków Za Pomocą Metod Instrumentalnychunclassified

Solid phase microextraction (SPME) as a modern technique for the isolation of medications from biological samples

Roszkowska¹

2018

Farm Pol.

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Section: Oznaczanie Leków Za Pomocą Metod Instrumentalnychunclassified

Solid phase microextraction (SPME) as a modern technique for the isolation of medications from biological samples

Roszkowska¹

2018

Farm Pol.

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“…An alternative method for the analysis of ionic species is CE. CE has recently been used for a variety of clinical applications and is a suitable method for monitoring metabolic inborn errors . CE has several advantages over standard chromatographic techniques in detecting diagnostic molecules, including superior separation efficiency, reduced reagent consumption, easier sample preparation (simple dilution) , and shorter analysis time.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis with capacitively coupled contactless conductivity detection for the determination of urinary ethylmalonic acid for the diagnosis of ethylmalonic aciduria

Özçelik

Öztekin

Kıykım

et al. 2020

J of Separation Science

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Ethylmalonic acid is a metabolic organic acid, and its accumulation in urine is diagnostic of ethylmalonic aciduria. In this study, a simple and fast method employing capillary electrophoresis equipped with capacitively coupled contactless conductivity detection was developed for the detection of ethylmalonic acid in urine samples. The optimized electrophoretic separation was performed in 50 mmol/L 2-(N-morpholino)ethanesulfonic acid solution, buffered at a pH of 6.5, and contained 0.13 mmol/L cetyltrimethylammonium bromide as an electroosmotic modifier. Electrophoresis was run at 28 kV in reversed polarity. The linear range of ethylmalonic acid concentration was between 1 and 100 mg/L with a regression coefficient of 0.9998. This method had good intra-and interday precision with <5% relative standard deviations. The detection limit (signal-to-noise ratio = 3) and the quantification limit (signal-to-noise ratio = 10) values were 0.139 and 0.466 mg/L, respectively. Using our optimized conditions, the method was successfully employed for the detection of ethylmalonic acid in urine sample of ethylmalonic aciduria patient. K E Y W O R D Sethylmalonic encephalopathy, inborn error, metabolic disorders, urine

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“…In the last years, instrumental advances on microchip electrophoresis (ME) systems have boosted the use of this platform for applications in different fields including clinical, bioanalytical, food, forensic and environmental sciences . Several outstanding reports have successfully addressed common problems associated with sample injection , integration of detectors as well as high‐throughput sample processing on chip‐based electrophoresis systems.…”

Section: Introductionmentioning

confidence: 99%

Separation of carbohydrates on electrophoresis microchips with controlled electrolysis

et al. 2019

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This study reports the separation of fructose, galactose, glucose, lactose and sucrose on glass microchip electrophoresis (ME) devices using a microfluidic platform adapted with external reservoirs for controlling the electrolysis phenomenon. The connections between external reservoirs and microfluidic platform were performed by saline bridges created using silicone tubing filled with BGE. The separation conditions were optimized and the best results were achieved using a BGE containing 75 mmol/L NaOH and 15 mmol/L trisodium phosphate. Electrophoretic separations were monitored using a capacitively coupled contactless conductivity detection system. The controlled electrolysis has successfully allowed the application of a higher voltage on the separation channel promoting the baseline separation of five carbohydrates within 180 s with great run‐to‐run repeatability (RSD < 1%). The achieved efficiencies ranged from 45 000 ± 6000 to 70 000 ± 3000 plates/m demonstrating a performance better than ME devices without controlled electrolysis. The proposed system offered good linearity from 1 to 10 mmol/L and LODs between 150 and 740 μmol/L. The use of external tubes for controlling the electrolysis phenomenon on ME devices has solved common problems associated to run‐to‐run repeatability and analytical reliability required for routine and quantitative analysis.

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Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Cited by 33 publications

References 138 publications

Solid phase microextraction (SPME) as a modern technique for the isolation of medications from biological samples

Solid phase microextraction (SPME) as a modern technique for the isolation of medications from biological samples

Capillary electrophoresis with capacitively coupled contactless conductivity detection for the determination of urinary ethylmalonic acid for the diagnosis of ethylmalonic aciduria

Separation of carbohydrates on electrophoresis microchips with controlled electrolysis

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