Simultaneous determination of diethylene glycol, diethylene glycol monoethyl ether, coumarin and caffeine in food items by gas chromatography

Rahim, Afidah Abdul; Saad, Bahruddin; Osman, Hasnah; Hashim, NoorHasani; Yahya, Saadiah; Talib, Khairuddin Md

doi:10.1016/j.foodchem.2010.11.121

Cited by 37 publications

(21 citation statements)

References 18 publications

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“…To the best of our knowledge, this study is the first to evaluate the contents of compounds in coffee and tea in Malaysia. The analysis of theobromine and caffeine in foods, biological fluids, environmental samples, plants, and water, was provided with different instrumental methods, such as highperformance liquid chromatography (HPLC) [4], gas chromatography-mass spectrometry-flame ionization detection (GC-MS-FID) [5], Fourier transform-infrared spectrophotometry (FT-IR) [6], near-infrared spectroscopy [7], UV-Vis spectrophotometry [8], FT-Raman spectrometry [9], and capillary electrophoresis (CE) [10]. Of these techniques, HPLC which offers advantages, such as simplicity and selectivity coupled with a UV-Vis detector, was deemed the preferred analysis system for the analysis of theobromine and caffeine [8,11,12].…”

Section: The Present Study Is Focused On the Analysis Of ------------mentioning

confidence: 99%

Determination of theobromine and caffeine in some Malaysian beverages by liquid chromatography-time-offlight mass spectrometry

Al-Qaim¹,

Yuzir²,

Mussa³

2018

Trop. J. Pharm Res

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To determine the concentration of theobromine (TB) and caffeine (CF) in tea and other beverages using liquid chromatography-time of flight-mass spectrometry (LC-TOF-MS). Methods: The extract of caffeine and theobromine from tea and other beverages was filtered by 0.45 μm nylon micro-syringe and then injected into a LC-ToF-MS system. Theobromine and caffeine were separated using Thermo Scientific C18-column (length 250 mm, width 2.1 mm and diameter 5 μm). Acetonitrile-methanol (ACN-MeOH, 3:1 v/v) was used as mobile phase B, while mobile phase A was 0.1 % FA in DIW. The volume injected was 30 μL at a rate of 0.3 mL/min. Results: Good linearity was obtained in the range of 0.3-400 and 0.2-200 mg/L for theobromine and caffeine, respectively (regression coefficient (R 2) > 0.970). The limits of detection were 0.15 and 0.05 μg/mL for theobromine and caffeine, respectively. The highest concentrations of caffeine and theobromine determined in tea samples were 159.1 and 255.8 mg/L, respectively. Conclusion: Theobromine and caffeine have been successfully analysed in tea, coffee and soft drinks. LC-TOF-MS is an accurate and promising instrument for the determination of the studied compounds in beverages.

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Section: The Present Study Is Focused On the Analysis Of ------------mentioning

confidence: 99%

Determination of theobromine and caffeine in some Malaysian beverages by liquid chromatography-time-offlight mass spectrometry

Al-Qaim¹,

Yuzir²,

Mussa³

2018

Trop. J. Pharm Res

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“…So far, a variety of analytical methods based on GC [20,21], GC-MS [22], HPLC [23][24][25][26][27], and LC-MS [28,29] have been developed to analyze coumarins, but the low accuracy, high organic reagent consumption and long analysis times of these conventional methods are primary obstacles because of the complex matrix effects of coumarins. In recent years, tandem mass spectrometry (MS/MS) has been reported to provide a much higher degree of assurance than the single stage mass spectrometry technique when determining analytes in many complex matrix [30].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

et al. 2016

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Abstract:In this paper an analytical method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the determination of coumarin and its derivatives in tobacco products was developed. The MS/MS fragmentation pathways of the eight coumarins were elucidated. The new analytical method was defined based on two main axes, an extraction procedure with acetonitrile and analyte detection performed by HPLC-MS/MS in electron impact mode. The excellent selectivity and sensitivity achieved in multiple reaction monitoring (MRM) mode allowed satisfactory confirmation and quantitation for the coumarin flavor additives. Under the optimized gradient elution conditions, it took only 4.5 min to separate all eight coumarins. Good linearity for all the analytes were confirmed by the correlation coefficient r 2 , ranging from 0.9987 to 0.9996. The limits of detection (LODs) and limits of quantitation (LOQs) of these compounds were in the range of 0.5-1.7 µg/kg and 1.7-5.2 µg/kg, respectively. The average recoveries at three spiked levels (LOQ, 1.5LOQ, 2LOQ) were all in the range of 69.6%-95.1% with RSDs (n = 6) lower than 5.3%. The method of HPLC-MS/MS developed in this study was initially applied to the research of coumarin flavor additives in tobacco products collected from the located market in Beijing from China and proved to be accurate, sensitive, convenient and practical.

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“…[4] Chromatographic techniques appear to be useful for identification and quantification of multicomponents. [5,6] However, chromatographic determinations often require tedious and time-consuming procedures of sample preparation, [7,8] which makes their real-time and online use impossible, especially in large numbers of samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Azorubin and New Red by Synchronous Fluorescence Spectra Coupled with Radial Basis Function Neural Networks

Kong

Zhu

et al. 2014

Spectroscopy Letters

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A method for the simultaneous determination of Azorubin and New Red was proposed, which used the synchronous fluorescence spectroscopy coupled with the radial basis function neural networks. The synchronous fluorescence spectra of 59 standards of Azorubin and New Red mixed with concentrations ranging from 5 to 20 mg mL À1 were obtained by synchronous scanning the excitation and emission monochromator maintained at an offset of 70 nm. A method of exploiting the complicated function relationship between the concentrations of Azorubin and New Red and the intensities of their mixtures was discussed according to the accuracies of the radial basis function neural networks predicting results. The final root mean square error is 0.1922 and the total mean relative error is 2.56%. Results show that the method developed is effective, simple, and of satisfactory accuracy.

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Simultaneous determination of diethylene glycol, diethylene glycol monoethyl ether, coumarin and caffeine in food items by gas chromatography

Cited by 37 publications

References 18 publications

Determination of theobromine and caffeine in some Malaysian beverages by liquid chromatography-time-offlight mass spectrometry

Determination of theobromine and caffeine in some Malaysian beverages by liquid chromatography-time-offlight mass spectrometry

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Determination of Azorubin and New Red by Synchronous Fluorescence Spectra Coupled with Radial Basis Function Neural Networks

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