Simultaneous determination of diclofenac sodium and paracetamol in a pharmaceutical preparation and in bulk drug powder by high-performance thin-layer chromatography

Dighe, Vidya; Sane, R. T.; Menon, S. N.; Tambe, Harsha N.; Pillai, Saju; Gokarn, Vijay N.

doi:10.1556/jpc.19.2006.6.7

Cited by 16 publications

(9 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different analytical methods for the determination of diclofenac sodium or potassium in drugs are currently known. These are: thin-layer chromatography [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18], high-performance liquid chromatography (HPLC) [19,20], ultra high performance liquid chromatography (UPLC) [21], gas chromatography [22], capillary electrophoresis [23], spectrofluorimetry [24,25], UV-Vis spectrophotometry [26,27], flow extraction [28], flame emission [29], potentiometry [30,31], voltammetry [22,32,33], scanning colorimetry [34] and the photometric method with diffuse reflection [35]. The American, European and Polish Pharmacopoeias recommend a titration method for the quantitative determination of diclofenac sodium in the pharmaceuticals [36][37][38].…”

Section: Introductionmentioning

confidence: 99%

“…The literature review indicates that TLC and HPTLC methods have been used for quantitative determination of diclofenac in both, i.e., simple and complex preparations [4,5,7,14]. Diclofenac sodium was analyzed in different pharmaceutical formulations together with lidocaine [6,7], misoprostol [7], salicylic acid and methyl salicylate [14], tolperisone hydrochloride [8], ibuprofen [9], acetaminophenone (paracetamol) [10,12,15], chlorzoxazone and acetaminophenone [16], chlorzoxazone, acetaminophenone and famotidine [17].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Application of Thin-Layer Chromatography in Combination with Densitometry for the Determination of Diclofenac in Enteric Coated Tablets

2019

View full text Add to dashboard Cite

Diclofenac belongs to the drug class non-steroidal anti-inflammatory drugs widely used in Europe as well as all over the world. Thus, it is important to conduct research on its quality control of available pharmaceutical preparations like for example enteric coated tablets. Among various analytical techniques, thin-layer chromatography (TLC) is ideal for this task due to their short time analysis, ease of operation and low cost. Hence, the aim of this study was to develop the optimal conditions of analysis and quantitative determination of diclofenac sodium in enteric tablets by using TLC in combination with densitometry. Of all chromatographic systems tested, the best is the one which consists of silica gel 60F254 and cyclohexane: chloroform:methanol:glacial acetic acid (6:3:0.5:0.5 v/v) as the mobile phase, which allows the successful separation of examined diclofenac sodium as active component and the largest number (twelve) of its degradation products as potential impurities of its pharmaceutical products. This indicates that the newly developed method is more effective than previously reported assays by Starek and Krzek. Linearity range was found to be 4.00–18.00 μg/spot for diclofenac sodium. The results of the assay of enteric tablet formulations equals 98.8% of diclofenac sodium in relation to label claim is in a good agreement with pharmaceutical requirements.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of Thin-Layer Chromatography in Combination with Densitometry for the Determination of Diclofenac in Enteric Coated Tablets

2019

View full text Add to dashboard Cite

show abstract

“…These methods include spectrophotometry and multivariate [4][5][6], fluorimetry [7,8], voltammetry [9,10], high-performance liquid chromatography-ultraviolet (HPLC-UV) [11][12][13][14][15], liquid chromatographytandem mass spectrometry (LC-MS/MS) [16][17][18][19], capillary zone electrophoresis [20], and densitometry [21,22]. Other methods were developed for the simultaneous determination of DIC in binary combination with PAR or CHZ that include spectrophotometry [23][24][25], densitometry [26], voltammetry [27], and HPLC-UV [28,29]. Some methods were reported for the simultaneous determination of ternary combination of the three drugs based on densitometry [30,31] and HPLC-UV [32,33].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Spectrophotometric Determination of Diclofenac Sodium, Paracetamol, and Chlorzoxazone in Ternary Mixture Using Chemometric and Artificial Neural Networks Techniques

El‐Bagary

Elkady

Al-matari

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: The aim of this study is to develop and validate simple, accurate, and precise spectrophotometric methods for the simultaneous determination of diclofenac sodium (DIC), paracetamol (PAR), and chlorzoxazone (CHZ) in ternary mixture using chemometric and artificial neural networks (ANN) techniques. Methods:Three chemometric techniques include classical least squares (CLS), principal component regression (PCR), and partial least squares (PLS) in addition to cascade-forward backpropagation ANN (CFBP-ANN) were prepared using the synthetic mixtures containing the three drugs in methanol. In CLS, PCR, and PLS, the absorbances of the synthetic mixtures in the range 267-295 nm with the intervals ∆λ=0.2 nm in their zero-order spectra were selected. Then, calibration or regression was obtained using the absorbance data matrix and concentration data matrix for the prediction of the unknown concentrations of DIC, PAR, and CHZ in their mixtures. In CFBP-ANN, two layers, sigmoid layer with 10 neurons and linear layer were found appropriate for the simultaneous determination of the three drugs in their ternary mixture. Results:The four proposed methods were successfully applied to the analysis of the three drugs in laboratory prepared mixtures and tablets with good percentage recoveries in the range of 98-102%. Relative standard deviation for the precision study was found <1%. Conclusion:The four proposed methods showed simplicity, accuracy, precision, and rapidity making them suitable for quality control and routine analysis of the cited drugs in ternary mixtures and pharmaceutical formulation containing them.

show abstract

“…The literature survey reveals that PCM is estimated individually or in combination with other drugs by UV [9][10][11][12], HPLC [13][14][15][16][17][18][19][20][21][22], and in plasma [23,24] by RP-HPLC. In addition, LC-MS [25,26], HPTLC [27][28][29][30][31][32][33], and stabilityindicating GC/MS method [34] have been reported.…”

Section: Introductionmentioning

confidence: 99%

Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form

Tandulwadkar

Nikam

et al. 2012

ISRN Chromatography

View full text Add to dashboard Cite

A simple, precise, and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous determination of paracetamol (PCM), chlorzoxazone (CHZ), and nimesulide (NIM) in pharmaceutical dosage form. The chromatographic separation was achieved on a Thermo Hypersil GOLD C18column (250 × 4.6 mm i.d., 5 μm particle size). The mobile phase consisted of water : acetonitrile (55 : 45 v/v). The flow rate was set to 1.2 mL min−1and UV detection was carried out at 275 nm. The retention time () for PCM, CHZ, and NIM was found to be 2.69 ± 0.02, 4.61 ± 0.01, and 9.55 ± 0.02 min, respectively. The validation of the proposed method was carried out for linearity, precision, robustness, limit of detection, limit of quantitation, specificity, and accuracy. The linear dynamic ranges were 32.5–65.0 μg mL−1for PCM, 37.5–75.0 μg mL−1for CHZ, and 10.0–20.0 μg mL−1for NIM. The developed method can be used for routine quality control analysis of titled drugs in pharmaceutical dosage form.

show abstract

Simultaneous determination of diclofenac sodium and paracetamol in a pharmaceutical preparation and in bulk drug powder by high-performance thin-layer chromatography

Cited by 16 publications

References 5 publications

Application of Thin-Layer Chromatography in Combination with Densitometry for the Determination of Diclofenac in Enteric Coated Tablets

Application of Thin-Layer Chromatography in Combination with Densitometry for the Determination of Diclofenac in Enteric Coated Tablets

Simultaneous Spectrophotometric Determination of Diclofenac Sodium, Paracetamol, and Chlorzoxazone in Ternary Mixture Using Chemometric and Artificial Neural Networks Techniques

Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form

Contact Info

Product

Resources

About