Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form

Abstract

A simple, precise, and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous determination of paracetamol (PCM), chlorzoxazone (CHZ), and nimesulide (NIM) in pharmaceutical dosage form. The chromatographic separation was achieved on a Thermo Hypersil GOLD C18column (250 × 4.6 mm i.d., 5 μm particle size). The mobile phase consisted of water : acetonitrile (55 : 45 v/v). The flow rate was set to 1.2 mL min−1and UV detection was carried out at 275 nm. The retention time () for PCM, CHZ, and NIM was found to be 2.69 ± 0.02, 4.61 ± 0.01, and 9.55 ± 0.02 min, respectively. The validation of the proposed method was carried out for linearity, precision, robustness, limit of detection, limit of quantitation, specificity, and accuracy. The linear dynamic ranges were 32.5–65.0 μg mL−1for PCM, 37.5–75.0 μg mL−1for CHZ, and 10.0–20.0 μg mL−1for NIM. The developed method can be used for routine quality control analysis of titled drugs in pharmaceutical dosage form.

Simultaneous Detection of Chlorzoxazone and Paracetamol Using a Greener Reverse-Phase HPTLC-UV Method

“…For simultaneous detection of CZN and PCT in combined dosage forms, several ultraviolet (UV)-based spectrometry methods have been reported [1,[8][9][10][11][12]. For simultaneous detection of CZN and PCT in various commercially available combined pharmaceutical preparations, a number of "high-performance liquid chromatography (HPLC)" methods are also reported [13][14][15][16][17][18][19][20].…”

“…For simultaneous detection of CZN and PCT in combined dosage forms, several ultraviolet (UV)-based spectrometry methods have been reported [1,[8][9][10][11][12]. For simultaneous detection of CZN and PCT in various commercially available combined pharmaceutical preparations, a number of "high-performance liquid chromatography (HPLC)" methods are also reported [13][14][15][16][17][18][19][20]. Ultra-high-performance liquid chromatography (UHPLC) and supercritical fluid chromatography methods have also been utilized for simultaneous detection of CZN and PCT in commercially available combined dosage forms [21,22].…”

Uso do eletrodo de diamante dopado com boro acoplado a um sistema BIA para determinação simultânea de princípios ativos em medicamentos

“…Alguns métodos analíticos têm sido relatados para a determinação simultânea de NIM e PAR, incluindo cromatografia em camada delgada [12], espectrofotometria UV-VIS [2, 13] e cromatografia em fase líquida [14][15][16][17][18].…”