Simultaneous determination of cytokinins by high performance liquid chromatography with resonance Rayleigh scattering and mechanism discussion

Zhang, Zhongying; Peng, Jingdong; Li, Shiyu; Peng, Huanjun; Wang, Xiang; Long, Dengying; Xian, Hong; Ni, Ranxi

doi:10.1039/c9an00872a

Cited by 8 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The residues were dissolved in 120 μL of the high‐performance liquid chromatography (HPLC) mobile phase and 30 μL was injected into the HPLC‐EC system. The analysis was following a method previously described (Zhang et al ., 2019 ).…”

Section: Methodsmentioning

confidence: 99%

OsCOMT, encoding a caffeic acid O‐methyltransferase in melatonin biosynthesis, increases rice grain yield through dual regulation of leaf senescence and vascular development

Huangfu

Chen

et al. 2022

Plant Biotechnology Journal

View full text Add to dashboard Cite

Summary Melatonin, a natural phytohormone in plants, plays multiple critical roles in plant growth and stress responses. Although melatonin biosynthesis‐related genes have been suggested to possess diverse biological functions, their roles and functional mechanisms in regulating rice grain yield remain largely unexplored. Here, we uncovered the roles of a caffeic acid O‐methyltransferase (OsCOMT) gene in mediating rice grain yield through dual regulation of leaf senescence and vascular development. In vitro and in vivo evidence revealed that OsCOMT is involved in melatonin biosynthesis. Transgenic assays suggested that OsCOMT significantly delays leaf senescence at the grain filling stage by inhibiting degradation of chlorophyll and chloroplast, which, in turn, improves photosynthesis efficiency. In addition, the number and size of vascular bundles in the culms and leaves were significantly increased in the OsCOMT‐overexpressing plants, while decreased in the knockout plants, suggesting that OsCOMT plays a positive role in vascular development of rice. Further evidence indicated that OsCOMT‐mediated vascular development might owe to the crosstalk between melatonin and cytokinin. More importantly, we found that OsCOMT is a positive regulator of grain yield, and overexpression of OsCOMT increase grain yield per plant even in a high‐yield variety background, suggesting that OsCOMT can be used as an important target for enhancing rice yield. Our findings shed novel insights into melatonin‐mediated leaf senescence and vascular development and provide a possible strategy for genetic improvement of rice grain yield.

show abstract

Section: Methodsmentioning

confidence: 99%

OsCOMT, encoding a caffeic acid O‐methyltransferase in melatonin biosynthesis, increases rice grain yield through dual regulation of leaf senescence and vascular development

Huangfu

Chen

et al. 2022

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…Seedling leaves were collected 1, 7, and 15 days after smoke treatment and preserved in a dry ice cooler for subsequent laboratory analysis. The malondialdehyde (MDA) content in the leaves was determined by the thiobarbituric acid (TBA) method (Morales and Munné-Bosch 2019), while the contents of zeatin (ZA) and abscisic acid (ABA) were determined by high-performance liquid chromatography (HPLC) (Ciha et al 1977;Zhang et al 2019).…”

Section: Determination Of Malondialdehyde and Plant Hormone Levelsmentioning

confidence: 99%

Nitrogen allocation in PM2.5 smoke-exposed plants: implications for ecosystem nitrogen cycling and stress response

Lin,

Ma,

Zhao

et al. 2023

fire ecol

View full text Add to dashboard Cite

Background With the increase in forest fire emissions, an increasing amount of nitrogen is released from combustibles and taken up by plant leaves in the form of PM2.5 smoke deposition. Concurrently, the stress from PM2.5 also disrupts the physiological processes of plants. This study aims to reveal the migration paths of N in combustibles in smoke and plants during forest fires and the stress response of plant leaves to smoke particle deposition. This study conducted a simulated smoke deposition treatment on Schima superba and Cunninghamia lanceolata, analyzing the changes in plant 15N content and stress-related products. Results The main findings include the following: (1) Nitrogen in combustibles can be transported to plant leaves via PM2.5 smoke during combustion and can be allocated and assimilated in various parts of the plant after being absorbed by the leaves. (2) The stress response of Schima superba to PM2.5 is less pronounced than that of Cunninghamia lanceolata. (3) Under PM2.5 stress, the correlation between nitrogen accumulation in the leaves of Schima superba and Cunninghamia lanceolata and their respective stress responses differs. Conclusions In forest fires involving different tree species, there are variations in the migration pathways of nitrogen and the stress effects of PM2.5 on leaves, with a significant correlation observed between leaf nitrogen accumulation and stress response. Graphical Abstract

show abstract

“…Traditional analytical methods for quantifying 6-BA is inclusive of high-performance liquid chromatography (HPLC) and liquid chromatography–tandem mass spectrometry (LC–MS/MS), , which display high accuracy and sensitivity (Table ). However, they require expensive equipment, professional personnel, and time-consuming sample treatments.…”

Section: Introductionmentioning

confidence: 99%

Authenticating Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay

Quan

Liu

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

6-Benzylaminopurine (6-BA), a plant growth regulator with cytokinin-like properties, was recently reported to be illegally used in bean sprouts to increase their commercial appearance. It is still nevertheless challenging to quickly detect this adulteration. In this work, four novel haptens (haptens 1−4) of 6-BA were rationally designed with computer-assisted modeling analysis and then synthesized for use as immunizing haptens to produce antibodies. One of two obtained antibodies showed high sensitivity and specificity toward 6-BA. Based on the most sensitive anti-6-BA antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was performed, which demonstrated a 50% inhibition concentration (IC 50 ) of 1.18 μg/L and a limit of detection of 0.075 μg/L. The average recoveries of this icELISA for 6-BA of spiked samples ranged from 87.2 to 95.0% with a coefficient of variation of less than 8.7%. Furthermore, the blind samples were detected simultaneously by the method and HPLC-MS/MS, and the results showed good agreement with each other. Therefore, the proposed icELISA can facilitate the rapid surveillance screening of adulterated 6-BA in sprout vegetables.

show abstract

Simultaneous determination of cytokinins by high performance liquid chromatography with resonance Rayleigh scattering and mechanism discussion

Abstract: A method of high performance liquid chromatography associated with resonance Rayleigh scattering (HPLC-RRS) was developed to detect three cytokinins, namely, 6-benzylaminopurine (BA), kinetin (KT) and zeatin (ZT).

Cited by 8 publications

References 37 publications

OsCOMT, encoding a caffeic acid O‐methyltransferase in melatonin biosynthesis, increases rice grain yield through dual regulation of leaf senescence and vascular development

OsCOMT, encoding a caffeic acid O‐methyltransferase in melatonin biosynthesis, increases rice grain yield through dual regulation of leaf senescence and vascular development

Nitrogen allocation in PM2.5 smoke-exposed plants: implications for ecosystem nitrogen cycling and stress response

Authenticating Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay

Contact Info

Product

Resources

About