Simultaneous determination of curcumin, tetrahydrocurcumin, quercetin, and paeoniflorin by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study

Yu, Weilan; Wen, Dingsheng; Cai, Dake; Zheng, Juntao; Gan, Haining; Liu, Xiaolin; Lao, Biaochang; Yu, Wei-Bang; Guan, Yanping; Zhong, Guoping

doi:10.1016/j.jpba.2019.04.033

Cited by 26 publications

(17 citation statements)

References 27 publications

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“…Alternatively, there are a wide variety of methods described in the literature for CUR determination. Several methods have been described for the simultaneous determination of CUR and other derivatives or antioxidant compounds such as quercetin [ 21 , 22 , 23 ].…”

Section: Resultsmentioning

confidence: 99%

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

et al. 2022

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Curcumin (CUR) and D-panthenol (DPA) have been widely investigated for wound-healing treatment. In order to analyse these two compounds from a dosage form, such as polymer-based wound dressings or creams, an analytical method that allows the quantification of both drugs simultaneously should be developed. Here, we report for the first time a validated high-performance liquid chromatographic (HPLC) method coupled with UV detection to quantify CUR and DPA based on the standards set by the International Council on Harmonization (ICH) guidelines. The separation of the analytes was performed using a C18 column that utilised a mobile phase consisting of 0.001% v/v phosphoric acid and methanol using a gradient method with a run time of 15 min. The method is linear for drug concentrations within the range of 0.39–12.5 μg mL−1 (R2 = 0.9999) for CUR and 0.39–25 μg mL−1 for DPA (R2 = 1). The validated method was found to be precise and accurate. Moreover, the CUR and DPA solution was found to be stable under specific storage conditions. We, therefore, suggest that the HPLC-UV method developed in this study may be very useful in screening formulations for CUR and DPA within a preclinical setting through in vitro release studies.

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Section: Resultsmentioning

confidence: 99%

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

et al. 2022

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“…Compounds 1 and 3 were definitely identified as daidzein (Raju et al, 2019) and taxifolin (Wang et al, 2009), which are isoflavones at m / z 253.0630 and 303.0634 [M − H] − . Based on their fragment ions and by comparison with the retention times, compound 15, 17, 20, 22, 27, 30, 35, 40 and 46 were identified as luteolin (Cheruvu et al, 2018), luteolin‐7‐O‐rutinoside (Yin et al, 2013), mauritianin (Yasukawa & Takido, 1987), rutin (Yang et al, 2012), apigenin‐7 ‐O‐ glucoside (Hvattum & Ekeberg, 2003), luteolin‐7 ‐O‐ glucoside (Ola et al, 2009), ligustroflavone (Pieroni, Pachaly, Huang, Van Poel, & Vlietinck, 2000), apigenin (Cheruvu et al, 2018) and quercetin (W. Yu et al, 2019), observed at m / z 285.0405, 593.1650, 739.2155, 609.1417, 739.2155, 431.1104, 447.0877, 723.2228, 269.0424, 301.03595 [M − H] − .…”

Section: Resultsmentioning

confidence: 99%

Analysis of chemical variations between raw and wine‐processed Ligustri Lucidi Fructus by ultra‐high‐performance liquid chromatography–Q‐Exactive Orbitrap/MS combined with multivariate statistical analysis approach

Zhang

Sun

Mao

et al. 2020

Biomedical Chromatography

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Ligustri Lucidi Fructus (LLF) is the dried and mature fruit of Ligubtrum lucidum Ait., which has the effect of nourishing the liver and kidney, brightening the eyes and promoting the growth of black hair. Wine‐processed LLF is commonly used in traditional Chinese medicine; however, the processing mechanisms are still unclear. Herein, a system data acquisition and mining strategy was designed to investigate the chemical profile differences between the raw and wine‐processed LLF, based on high‐performance liquid chromatography–Orbitrap high resolution mass spectrometry coupled with multivariate statistical analysis including principal component analysis and partial least square analysis. Afterwars, a total of 55 components were found to be the main contributors to the significant difference between raw and wine‐processed LLF by comparison with chromatographic behaviors, intact precursor ions, and characteristic MS fragmentation patterns. In addition, 10 main constituents of raw and wine‐processed LLF were simultaneously determined by UHPLC–MS/MS for analyzing the content variations. Some structural transformation mechanisms during wine processing were deduced from the results. The results may provide a scientific foundation for deeply elucidating the wine‐processing mechanism of LLF.

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“…Very few methods for simultaneous estimation of curcumin and quercetin have been reported. [29][30][31] Advanced methods such as ultraperformance liquid chromatography-mass spectrometry (UPLC MS-MS) have been used for simultaneous determination of curcumin and quercetin in rat plasmas. However, this method is complex and requires costly instrumentation.…”

Section: Introductionmentioning

confidence: 99%

“…However, this method is complex and requires costly instrumentation. 29 Moreover, the HPLC method has been reported for the simultaneous determination of curcumin and quercetin. Thus, the limitation of this method is a long run time (20 minutes).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of RP-HPLC Method for Simultaneous Determination of Curcumin and Quercetin in Extracts, Marketed Formulations, and Self-Nanoemulsifying Drug Delivery System

et al. 2021

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Background: Curcumin and quercetin are the two important flavonoids that are being used as food ingredients across the globe. They also possess many pharmacological activities such as anti-inflammatory, anticancer and antidiabetic activity. The combination of both these phytoconstituents is available in the market. However, there is lack of any suitable analytical method reported for their simultaneous estimation in food as well as pharmaceutical products. Hence, there is a need to develop a simultaneous method for them. Objectives: The study aims to develop and validate a simple high-performance liquid chromatography (HPLC) method for the simultaneous determination of curcumin and quercetin. Method: Gradient elution was carried out in ACN and GAA (2% v/v) for 15 min wherein the ratio of ACN and GAA was varied from 40-60 v/v ACN: 60-40 GAA v/v from 0-5 min followed by 60-80 v/v ACN: 40-20 v/v GAA between 7-10 min followed by 80 % ACN: 20 % GAA between 10-15 min. The flow rate was kept 1 mL/min and detection wavelength at 395 nm. The method has been validated according to International Conference of Harmonization (ICH) Q2 (R1) guidelines with respect to specificity, system suitability, accuracy, precision, and robustness. The developed method was further used for the determination of curcumin and quercetin in different herbal extracts, marketed formulations containing these drugs and developed self-nano emulsifying drug delivery system (SNEDDS). Results: The method was linear in the concentration range of 50-250 ng mL1 for curcumin and 800-1600 ng mL À1 for quercetin, respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 11.76 and 35.6 ng mL À1 for curcumin, respectively, while LOD and LOQ for quercetin were 32.94 and 99.76 ng/mL À1 , respectively. The percentage recovery that was found to be in the range of 95-105% with relative standard deviation less than 2% indicating the accuracy and precision of method for both the drugs. Further, the validated method was found specific to detect presence of both the drugs in extracts, marketed formulations and developed SNEDDS. Conclusions: The developed method showed excellent specificity, linearity, accuracy and precision. Thus, it can be further explored to detect curcumin and quercetin in biological samples as well as other marketed formulations.

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Simultaneous determination of curcumin, tetrahydrocurcumin, quercetin, and paeoniflorin by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study

Cited by 26 publications

References 27 publications

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

Analysis of chemical variations between raw and wine‐processed Ligustri Lucidi Fructus by ultra‐high‐performance liquid chromatography–Q‐Exactive Orbitrap/MS combined with multivariate statistical analysis approach

Development and Validation of RP-HPLC Method for Simultaneous Determination of Curcumin and Quercetin in Extracts, Marketed Formulations, and Self-Nanoemulsifying Drug Delivery System

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