A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings

Abstract

Curcumin (CUR) and D-panthenol (DPA) have been widely investigated for wound-healing treatment. In order to analyse these two compounds from a dosage form, such as polymer-based wound dressings or creams, an analytical method that allows the quantification of both drugs simultaneously should be developed. Here, we report for the first time a validated high-performance liquid chromatographic (HPLC) method coupled with UV detection to quantify CUR and DPA based on the standards set by the International Council on Harmonization (ICH) guidelines. The separation of the analytes was performed using a C18 column that utilised a mobile phase consisting of 0.001% v/v phosphoric acid and methanol using a gradient method with a run time of 15 min. The method is linear for drug concentrations within the range of 0.39–12.5 μg mL−1 (R2 = 0.9999) for CUR and 0.39–25 μg mL−1 for DPA (R2 = 1). The validated method was found to be precise and accurate. Moreover, the CUR and DPA solution was found to be stable under specific storage conditions. We, therefore, suggest that the HPLC-UV method developed in this study may be very useful in screening formulations for CUR and DPA within a preclinical setting through in vitro release studies.

Fluorescence-Coupled Techniques for Determining Rose Bengal in Dermatological Formulations and Their Application to Ex Vivo Skin Deposition Studies

“…The precision, which indicates the method’s repeatability, exhibited an RSD% value from 0.98 to 3.06%, within the concentration range tested. The results confirmed that the new analytical method is accurate and precise since the values obtained were lower than the acceptable value of 15%; hence, they are under the recommendations for bioanalytical methods [ 60 , 66 , 67 ]. Based on these results, it can be assumed that the current approach is potentially appropriate for quantifying RB deposited into skin samples.…”

Bioadhesive eutectogels supporting drug nanocrystals for long-acting delivery to mucosal tissues

“…The in vitro release profiles of coarse CUR, PM, CUR NCs and Egel-20-NCs were assessed using a dialysis membrane model according to a previously described methodology [ 27 , 28 ]. The release media consisted of 100 ​mL of 2% w/v Tween® 80 in buffer saliva pH 6.4 containing 0.5% w/v of ascorbic acid to prevent drug degradation [ 29 ]. For this experiment, 2.6 ​mg of CUR powder and CUR NCs (containing 1.97 ​mg of CUR) were placed in dialysis membrane bags of 12,000–14,000 ​kDa molecular weight cut-off (MWCO) (Spectra-Por, Spectrum Medical Industries, Los Angeles, CA, U.S.A.) and sealed at both ends with clips.…”

An isocratic RP-HPLC-UV method for simultaneous quantification of tizanidine and lidocaine: application to in vitro release studies of a subcutaneous implant

“…Metrics like relative standard deviation (RSD) (eqn (3)), repeatability, and intermediate precision were computed based on the estimated concentrations. 55 For concentrations under analysis, an RSD value lower than 10% is considered acceptable. 52,56 …”