Aims: Since the end of the nineteenth century, when Werner proposed his theory of co-ordination, the study of metallic compounds has continued to attract the interest of researchers. The present work had as its aim to achieve simultaneous determination of Pb(II), Cu(II) and Ni(II) by means of UV-VIS and derivative spectrophotometry, using methyl-ethylene-diamine-tetraacetic acid, MEDTA, as a chelating agent. Study Design: The visible and ultraviolet spectroscopy allows both to identify a chemical substance (qualitative analysis) and to determine its concentration (quantitative analysis). In the work, the UV-VIS and derivative spectrophotometry is used for the determination simultaneous of Pb(II), Cu (II) and Ni (II). The method proposed uses methyl-ethylene-diamine-tetracetic acid (MEDTA) as a chelating agent and absorption spectrophotometry for quantification.