Simultaneous Determination of Caffeine, Catechin, Epicatechin, Chlorogenic and Caffeic Acid in Cola nitida Dried Nuts from Côte d’Ivoire Using HPLC

Yves, Nyamien; Coulibaly, Adama; Belleville, Marie‐Pierre; Petit, Eddy; Adima, Augustin Amissa; Biego, Godi Henri Marius

doi:10.9734/ajb2t/2017/34800

Cited by 9 publications

(12 citation statements)

References 13 publications

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“…This more or less significant presence is due to a post-harvest contamination by human or during kolanuts transport and storage [30]. Since the nuts after maturation and harvest are contained in the pods and coated with a thick protective membrane [19]. According to some authors, the low water solubility and the relatively high octanol/water partition coefficient (Kow) of PAHs give them a high adsorption potential on airborne particles in water and food [31,32].…”

Section: Validation Parameters For Pah Quantificationmentioning

confidence: 99%

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Evaluation of Effective and Safe Extraction Method for Analysis of Polycyclic Aromatic Hydrocarbons in Kolanuts from Côte d’Ivoire

Rodrigue

Biego

Bleouh

et al. 2020

AFSJ

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Aims: The current study targets the achievement of a reliable process for the determination of PAH contents in kola nuts for better appreciation of the risks incurred from the consumption of such food products. Study Design: Kolanuts collected from two big storage centers were analyzed after the validation of the proposed analytical method. Place and Duration of Study: Central Laboratory for Food Hygiene and Agro-Industry, LANADA in Abidjan, Côte d’Ivoire, running 2018. Methodology: Two references were used for the validation of the analytical method, namely the French standard NF V 03-110 and the ISO directive ISO/DIS/15753 applicable in Liquid Chromatography. The PAH contents of some samples collected from different stores were then determined. Results: From the data, a significant regression chart was recorded for the PAH detection graphs. All the analysis exhibited good linearity with significant correlation coefficients (R²˃ 0.99). The relative standard deviations of the repeatability and reproducibility assays are below 3%, whereas standard additions of PAH are fully recovered, with percentages close to 100%. Conclusion: Using this analytical method, kola nuts contamination by PAH have been determined with satisfactory.This analytical method could help in ensuring effective sanitary control at different critical points of kola nut distribution channel for promoting a good management of the toxicity concerns in such products.

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Section: Validation Parameters For Pah Quantificationmentioning

confidence: 99%

“…Kola nuts have an increasing interest for industries, mainly because of their richness in bioactive and functional compounds such as polyphenols, caffeine and theobromine [18,19]. They constitute an important raw material in the formulation of pharmaceutical, food, cosmetic and textile products [20,21,19].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Effective and Safe Extraction Method for Analysis of Polycyclic Aromatic Hydrocarbons in Kolanuts from Côte d’Ivoire

Rodrigue

Biego

Bleouh

et al. 2020

AFSJ

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“…Some of these phenolic acids include caffeic acid, chlorogenic acid, gallic acid, ferulic acid and the flavonoids are Quercetin and Rutin. In a previous study, (Nyamien et al, 2017) reported that cola nitida (kola nut), contain caffeic acid, chlorogenic acid and others compounds, while quercetin and other compounds were reported in G. kola (Bitter kola) (Buba et al, 2016). However, there is paucity of data on the presence of these phenolics in B. coriacea (wonderful kola), secondly the amount of these compounds present in each of this kola are not documented.…”

Section: Introductionmentioning

confidence: 99%

Quantification of some phenolics acids and flavonoids in Cola nitida, Garcinia kola and Buchholzia coriacea using high performance liquid chromatography- diode array detection (HPLC-DAD)

Adamu¹,

Ingbian²,

Okhale³

et al. 2020

J. Med. Plants Res.

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Kola seeds are highly valued in most African communities due to their medicinal benefits and sociocultural application during ceremonies. Apart from their neuro-stimulatory effect, the other health benefits such as antioxidant activities which are closely associated with the level of phenolic constituents have also been widely reported. The main purpose of this study is to determine the quantity of quercetin, caffeic acid, gallic acid, ferulic acid, rutin and chlorogenic acid in Cola nitida, Garcinia kola and Buchholzia coriacea using HPLC-DAD. Ethanolic extracts of the selected kola plants was obtained by cold maceration and analysed by HPLC-DAD in accordance with standard methods. The regression coefficient (r 2) from the calibration curve for caffeic acid = 0.999, chlorogenic acid = 0.998, gallic acid = 0.999, ferulic acid = 0.998, quercetin = 0.996 and rutin = 0.997. The repeatability gave % RSD of 0.6, 1.63, 0.44, 1.55, 3.65 and 4.67 for caffeic acid, chlorogenic acid, gallic acid, ferulic acid, quercetin and rutin respectively. The quantity of these compounds in C. nitida was caffeic acid (101.24 mg/g), chlorogenic acid (36.35 mg/g), gallic acid (16.99 mg/g) ferulic acid (1.47 mg/g) while quercetin and rutin were not detected. In Garcinia kola, caffeic acid was (0.84 mg/g), gallic acid (1.02 mg/g), ferulic acid (21.83 mg/g), quercetin (53.24 mg/g), rutin (0.49 mg/g) and chlorogenic acid was not detected. B. coriaceae had caffeic acid (1.03 mg/g), chlorogenic acid (0.33 mg/g), gallic acid (1.07 mg/g) while ferulic acid, quercetin and rutin were not detected. Using this analytical method, the quantities of some phenolics and flavonoids compounds were determined, and the most abundant compound in the three species of kola was caffeic acid in C. nitida and quercetin in Garcinia kola. This study also showed that C. nitida contains high amounts of phenolics compounds as compare to the other species of kola seeds investigated in the study.

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“…The importance of kolanuts is relative to its chemical composition. Indeed, it is a source of bioactive and functional compounds such as polyphenols and caffeine which reflects an increasingly growing interest for industries [26,27]. Then, nowadays, one of the serious problems in terms of safety of kalonut is the presence of PAHs [6,7].…”

Section: Introductionmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons Kolanuts (Cola nitida Schott & Amp; Endl) Daily Intake Exposure Risk from Côte d’Ivoire, West Africa

Rodrigue

Biego

Yves

et al. 2020

JALSI

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Polycyclic aromatic hydrocarbons (PAHs) are pervasive environmental pollutants of high toxicity. Due to their lipophilic characteristics, they tend to accumulate in the food chain. Human exposure to PAHs seems inevitable and the main route of exposure is food. The presence of PAHs in kolanut could cause serious health problem for consumers and slow down the export to new markets, which would constitute a significant shortfall for all actors in the kola sector.This study aimed to detrmined the Polycyclic Aromatic Hydrocarbons (PAHs) levels in kolanuts and estimate the exposure risks of nuts consumption by Ivorian population. Samples were collected from farmers, rural collectors, urban stores in districts (Mountains, Comoe, Lagoons, Down-Sassandra) and big storage centers of Anyama and Bouake cities. Concentrations of 9 PAHs (B[a]P, B[b]F, B[a]A, CHR, FLA, B[k]F, D[ah]A, B[ghi]P and IcdP) were measured using an Adept brand High Performance Liquid Chromatograph (HPLC) equipped with an ultraviolet (UV) / visible CE 4200 (CECIL) detector. Data showed the average concentration of PAHs in kolanuts at 1.22 ± 0.86 µg/kg. The PAHs concentrations expressed in BaP equivalent (BaPeq) ranging from 0.0009 ± 0.0003 µg/kg-BaPeq to 0.88 ± 0.24 µg/kg-BaPeq for B[ghi] P and D[ah]A. Based on the concentrations and the daily consumption of kolanuts estimated at 0.6 g/person in Côte d'Ivoire, the intakes values estimated of PAHs vary from 6.10-5 µg-BaPeq /day to 6.48.10-4 µg-BaPeq/day with an average of 3.06.10-4 ± 6.6.10-5 µg-BaPeq/day. The exposure daily doses (EDD) are all lower than the toxicity reference values (5 ng-BaPeq/kg BW/d). Thus, the occurrence of a toxic effect from PAHs after kolanuts consumption is very unlikely since the hazard quotient (HQ) are all less than 1. The risk of developing cancer is less than one case per 1,000,000 people.

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Simultaneous Determination of Caffeine, Catechin, Epicatechin, Chlorogenic and Caffeic Acid in Cola nitida Dried Nuts from Côte d’Ivoire Using HPLC

Cited by 9 publications

References 13 publications

Evaluation of Effective and Safe Extraction Method for Analysis of Polycyclic Aromatic Hydrocarbons in Kolanuts from Côte d’Ivoire

Evaluation of Effective and Safe Extraction Method for Analysis of Polycyclic Aromatic Hydrocarbons in Kolanuts from Côte d’Ivoire

Quantification of some phenolics acids and flavonoids in Cola nitida, Garcinia kola and Buchholzia coriacea using high performance liquid chromatography- diode array detection (HPLC-DAD)

Polycyclic Aromatic Hydrocarbons Kolanuts (Cola nitida Schott & Amp; Endl) Daily Intake Exposure Risk from Côte d’Ivoire, West Africa

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