Simultaneous determination of bioactive flavonoids in some selected Korean thistles by high-performance liquid chromatography

Abstract

In order to facilitate the quality control of some selected Korean thistles (Cirsii Herb), Cirsium japonicum var ussuriense, C. japonium var spinosissimum, C. setidens, C. pendulum, C. nipponicum, Carduus crispus, and Breea segetum, a simple, accurate and reliable high performance liguid chromatography method was developed for the simultaneous determination of the six flavonoids: luteolin 5-O-glucoside (1), luteolin 7-O-glucoside (2), hispidulin 7-O-neohesperidoside (3), luteolin (4), pectolinarin (5), and apigenin (6), which were selected as the chemical markers of the thistles. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-methanol at a flow-rate of 1.0 mL/min and detected at 254 nm. All six calibration curves showed good linearity (R(2) > 0.991). The method was reproducible with intra- and inter-day variations of less than 6%. The recoveries were in the range of 90.01-100.05%. This analysis method was successfully utilized to quantify the six flavonoids in the 22 batches of the thistles. The results demonstrated that this method is simple, reliable and suitable for the quality control of this medicinal herb.

De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids

“…Octadecadienoic acid, accounting for about 80% of safflower seed oil, can regulate cholesterol and is helpful in preventing cardiovascular disease [2], [3]. The major bioactive compound in safflower petals is flavonoid, which reportedly has many different pharmacological effects including preventing the occurrence of oxidation, inflammation, hypertension and cancer, and promoting blood circulation to dredge collaterals [4], [5]. In addition, safflower petals are commercially produced for use in the coloring and flavoring of foods, and for making dyes.…”

HPLC‐ESI‐MS/MS analysis and pharmacokinetics of luteoloside, a potential anticarcinogenic component isolated from Lonicera japonica, in beagle dogs

“…As part of the ongoing investigation of this new biologically active compound from medicinal herbs, pharmacokinetic studies based on qualified bioanalytical methods are very important for further development of luteoloside. Previous assays have described several methods for the determination of leutoloside in all kinds of plants and medicinal herbs, including Micellar Electrokinetic Chromatography (MEC) (Zhang et al ., ), Capillary Chromatography (CE) (Luo et al ., ; Fonseca et al ., ), High‐Performance Liquid Chromatography with UV Detection (HPLC‐UV) (Qian et al ., , ; Thao et al ., ; Qiao et al ., ; El‐Hela et al ., ) and LC‐MS (Liquid Chromatography with Mass Spectrometric Detection) (Lin et al ., ; Xu et al ., ; Plazonić et al ., ; Qian et al ., a, b) methods. However, so far there are no published methods for the bioassay of luteoloside in biological fluids.…”

Biological activities of a garlic–Cirsium setidens Nakai blend fermented with Leuconostoc mesenteroides

“…It is a perennial herb belonging to the family Compositae and is distributed mainly in the Gangwon Province of Korea (Cho et al, ). The plant has been used for treating edema, bleeding, and hemoptysis since it contains bioactive compounds such as hispidulin 7‐O‐neohesperidoside, pectolinarin, luteolin, and apigenin (Thao et al, ). Earlier studies have proven the biological effects of C. setidens Nakai, including antioxidant properties (Lee, Heo, Li, Lee, & Wang, ), hepatoprotective activities (Yoo, Nam, Kim, Choi, & Park, ), and activity against nonalcoholic fatty liver disease (Noh et al, ).…”