HPLC‐ESI‐MS/MS analysis and pharmacokinetics of luteoloside, a potential anticarcinogenic component isolated from Lonicera japonica, in beagle dogs

Abstract

Luteoloside is a potential anticarcinogenic component isolated from Lonicera japonica, a traditional Chinese medicine (TCM). This study details the development and validation of a sensitive and accurate HPLC-ESI-MS/MS method for the quantification of luteoloside in dog plasma. Sample pretreatment includes simple protein precipitation using methanol-acetonitrile (1:1, v/v). A Phenomenex Gemini C(18) column (2.0 × 50 mm, i.d., 3.5 µm) was used to separate luteoloside and internal standard by gradient mode with mobile phase consisting of water containing 0.1% formic acid and methanol containing 0.1% formic acid at a flow rate of 0.40 mL/min with a column temperature of 25°C. The detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring mode. The calibration curves were linear (R > 0.995) over the concentration range 1.0-2000 ng/mL and the lower limit of quantification was 1.0 ng/mL. The intra-day and inter-day precisions (RSD) were all <15%, accuracies (RE) were within the range of ±15%, and recoveries were between 85.0 and 115%. The validated HPLC-ESI-MS/MS method was successfully applied to determine plasma concentrations of luteoloside after intravenous administration of luteoloside at a dose level of 20 mg/kg.

Luteoloside Suppresses Proliferation and Metastasis of Hepatocellular Carcinoma Cells by Inhibition of NLRP3 Inflammasome

“…Flavonoids are well known as ROS scavengers. As luteoloside is a kind of flavonoid isolated from Chinese herb [30], we investigated whether the intracellular ROS is part of the mechanism by which luteoloside suppress the proliferation, migration and invasion potential of HCC cells. We found that luteoloside could significantly decrease the ROS level of Huh-7 and SMMC-7721 cells in a dose-dependent manner (Fig.…”

A nanocomposite consisting of graphene oxide and Fe3O4 magnetic nanoparticles for the extraction of flavonoids from tea, wine and urine samples

“…In order to further demonstrate the superiority of our method, we compared our method with previous reported [1,2,6,8,35,36]. As seen from Table 4, in comparison with other methods, MSPE method had higher extraction ability, thereby it illustrated our method not only had better extraction efficiency but also shortened extraction time to a great extent.…”

“…Several methods have been reported for the determination of flavonoids in different matrices, including HPLC-ESI-MS/ MS in traditional Chinese medicine (Lonicera japonica) [6] and in red wine [7]. HPLC-UV in Echinophora platyloba [8], HPLC-UV in plasma [9] and urine [10], HPLC with fluorometric detection in plasma and urine [11], and a potential method [12].…”

Lonicera japonica Thunb 金银花 (Jinyinhua, Honey Suckle)

“…Researchers thought most of these effects may be related to the active compositions of volatile oil, chlorogenic acid, and flavones. Chlorogenic acid and luteoloside, officially used as the indicator compound to characterize the quality of this herb and its related preparations, were shown to have beneficial effects in the aspects of anti-oxidation and antitumor [1,13]. Moreover, chlorogenic acid showed the antibacterial, antiviral, anti-inflammatory, hypoglycemic activities, and allergy-preventive properties [1,14].…”