Simultaneous Determination of Atenolol and Chlorthalidone by LC–MS–MS in Human Plasma

Khuroo, Arshad; Mishra, Sanjeev; Singh, Onkar; Saxena, Saurabh; Monif, Tausif

doi:10.1365/s10337-008-0755-7

Cited by 31 publications

(20 citation statements)

References 18 publications

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“…These values were consistent with those reported in the literature in pharmacokinetic studies [10,13,18,38] indicating that the method is suitable for routine monitoring of the considered drugs. Additionally, plasma samples were also analysed by another validated method previously developed in our laboratory [39].…”

Section: Application To Plasma Samplessupporting

confidence: 91%

Development of a MSFIA sample treatment system as front end of GC–MS for atenolol and propranolol determination in human plasma

Brunetto

Clavijo

Delgado

et al. 2015

Talanta

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Section: Application To Plasma Samplessupporting

confidence: 91%

Development of a MSFIA sample treatment system as front end of GC–MS for atenolol and propranolol determination in human plasma

Brunetto

Clavijo

Delgado

et al. 2015

Talanta

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“…Also, the run time of the method was only 2.4 min with the use of UPLC, which further increased the throughput. Various methods are reported in literature for the quantitation of amodiaquine (Stepniewska et al 2009), chloroquine (Hodel et al 2009;Singhal et al 2007) and chlorthalidone (Khuroo et al 2008;Salvadori et al 2009) in plasma. However, all the reported methods use time and resource-intensive extraction methods or have a long run time.…”

Section: Discussionmentioning

confidence: 99%

A modified serial blood sampling technique and utility of dried-blood spot technique in estimation of blood concentration: application in mouse pharmacokinetics

Kurawattimath

Pocha

Mariappan

et al. 2011

Eur J Drug Metab Pharmacokinet

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Pharmacokinetic (PK) studies in mice usually require discrete and parallel blood sampling owing to a restriction on the volume of blood that can be withdrawn. This results in dosing large number of animals and generating composite PK profile. To reduce the number of animals and generate individual animal PK profiles, we developed a serial sampling technique via tail vein bleeding in mice, in which only 20-30 μL blood was withdrawn per time point. Due to the small blood volume, a dried-blood spot (DBS) technique was applied for sample processing. The utility of this technique was demonstrated using three test compounds (amodiaquine, chloroquine and chlorthalidone), with varying degrees of blood-to-plasma partition ratios. The PK studies were carried out in male Balb/c mouse weighing 25-30 g. The compounds were administered intravenously via the saphenous vein. Blood was collected by composite (retro-orbital plexus) or serial (tail vein bleeding) sampling techniques at different time points. Blood samples were processed as blood lysate or DBS. Blood or plasma samples were analyzed by sensitive and rapid UPLC-MS/MS methods. The blood concentrations (both from blood lysate and DBS) obtained from serial sampling matched with those from composite sampling. The ratio of blood AUC to plasma AUC correlated well with the in vitro blood-to-plasma partition ratio of the compounds. The systemic clearance and volume of distribution at steady state calculated from blood or plasma AUCs were in proportion to the respective AUCs. Our results indicated that the serial sampling technique would reduce the number of animals and also compound usage, as well as improve the quality of pharmacokinetic data. Also, the serial sampling technique does not require the use of anesthesia and allows estimation of inter-animal variability in PK. A small volume serial sampling is possible due to the availability of the DBS technique.

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“…Literature survey reveals bioanalytical methods by liquid chromatography-mass spectrometry (LC-MS) for detection of chlorthalidone in human serum and blood, few spectrophotometric methods, and HPLC methods for the quantitative estimation of chlorthalidone in bulk and pharmaceutical formulations. [9][10][11][12] Currently, most commonly prescribed medicines for cardiovascular diseases are statins and diuretics. The present drug combination has promising effect to control hypertension and other heart diseases.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous estimation of metoprolol succinate and chlorthalidone in pharmaceutical solid dosage form by using a developed and validated reverse phase high performance liquid chromatographic technique

Sheth¹,

Patel²,

Ramlingam³

et al. 2012

Scho Res J

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The separation of both the drugs was achieved on Inertsil ODS 3 column (100 × 4.6 mm, 5 µm) column using a mobile phase of diammonium hydrogen phosphate buffer solution (at pH 5.5): Methanol (70:30 v/v). The flow rate was 1.0 ml/min and detection was done at 254 nm. The retention time for metoprolol succinate was 6.91 min and chlorthalidone was 9.94 min. Metoprolol succinate and chlorthalidone showed a linear response in the concentration range of 50-300 µg/ml and 12.5-75 µg/ml, respectively. The correlation coefficients for metoprolol succinate and chlorthalidone were 0.9999 and 0.9998, respectively. The percentage recoveries obtained for metoprolol succinate and chlorthalidone ranges from 98.9 to 100.6% and 98.5to 99.2%, respectively. The results of analysis have been validated as per International Conference on Harmonization (ICH) guidelines. Validation results indicated that method shows satisfactory linearity, accuracy, precision, and ruggedness. The extremely low flow rate, simple mobile phase composition makes this method cost effective, rapid, and nontedious and can also be successfully employed for simultaneous estimation of both drugs in commercial products.

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Simultaneous Determination of Atenolol and Chlorthalidone by LC–MS–MS in Human Plasma

Cited by 31 publications

References 18 publications

Development of a MSFIA sample treatment system as front end of GC–MS for atenolol and propranolol determination in human plasma

Development of a MSFIA sample treatment system as front end of GC–MS for atenolol and propranolol determination in human plasma

A modified serial blood sampling technique and utility of dried-blood spot technique in estimation of blood concentration: application in mouse pharmacokinetics

Simultaneous estimation of metoprolol succinate and chlorthalidone in pharmaceutical solid dosage form by using a developed and validated reverse phase high performance liquid chromatographic technique

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