Simultaneous Determination of Ascorbic Acid and Acetylsalicylic Acid in Pharmaceutical Formulations

Abstract

A direct, simple, and practical first-derivative spectrophotometric method is described for simultaneous determination of ascorbic acid and acetylsalicylic acid. The effects of the solvent, excipients, and spectral variables on the analytical signal were investigated. The drugs were determined simultaneously with a 0.01M methanolic hydrochloric acid solution as the solvent, and the signals were evaluated directly by using the zero-crossing method at 245.0 and 256.0 nm for acetylsalicylic acid and ascorbic acid, respectively. The method allows the simultaneous determinations of acetylsalicylic acid and ascorbic acid in the ranges of 6.6 × 10−6 to 1.5 × 10−4M and 3.4 × 10−6 to 2.0 × 10−4M, respectively, with standard deviation of <2.0%. The proposed method was applied to determinations of these drugs in tablets.

Ascorbiс Acid Degradation in N, N-Dimethylformamide Solutions

“…It is known [1,25,26] that in the presence of trace amounts of water, DMF slowly hydrolyzes to form dimethylamine:…”

“…The processes described in the article can occur during the synthesis of pharmaceutical substances, their quality control and in lipophylic sites in vivo. Vitamin C in the human organism can undergo degradation by mechanisms different from the aquatic environment [25,31]. In mammals, the non-enzymatic interaction that binds amines with carbonyl groups of various compounds (Maillard reaction) can lead to the formation of multicomponent mixtures of various chiral products.…”

Development and Validation of Rp-Uplc and Derivative Spectrophotometric Methods for Quantitative Determination of Ascorbic Acid and N-Acetylcysteine in Effervescent Tablets

“…There are also several quantitative analytical methods with liquid chromatography for these two active ingredients with the combinations of other active ingredients. There are several HPLC quantitative methods developed for the only AA 1,[4][5][6] and for only NAC 3,7 and also several spectroscopic quantitative methods developed for only AA 2,[8][9][10][11][12][13][14] , HPLC methods for AA [15][16][17][18][19][20][21][22] and NAC [23][24][25] in combination with other active ingredients, derivative spectroscopy method for only AA 26,27 and AA [28][29][30][31][32][33][34][35] in combination with other active ingredients. On the other hand, there are no spectroscopic or derivative spectroscopic quantity determination works that have been reported for NAC only.…”

Simultaneous Determination of Paracetamol, Acetyl Salicylic Acid, Mefenamic Acid and Cetirizine Dihydrochloride in the Pharmaceutical Dosage Form

“…Reversed phase liquid chromatographic method is preferred over other techniques for routine analysis of drug as it is less time consuming and also economical. A literature survey regarding quantitative analysis of these drugs revealed that attempts were made to develop analytical methods for paracetamol, acetyl salicylic acid, mefenamic acid and cetirizine dihydrochloride using spectrophotometry [1][2][3][4][5][6][7][8][9][10][11][12][13] , titration 14 , HPTLC 15 , disintegration test 16 , dissolution test 17 , FTIR, DSC and XRD 18 , IR and NMR 19 , HPLC [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] , LC -eletrospray ionization MS 38 , LC -MS/MS 39 and micellar electrokinetics capillary chromatography 40 . Paracetamol, acetyl salicylic acid, mefenamic acid and cetirizine dihydrochloride is official in pharmacopoeias and HPLC method had been reported for its estimation.…”