Simultaneous determination of anions and triclosan in dentifrices by gradient ion chromatography and isocratic high- performance liquid chromatography interfaced with conductivity and ultraviolet detection

Demkowicz, Michael P.; Chauhan, Varsha; Stern, David I.; Vasquez, Fred G.

doi:10.1016/0021-9673(94)80260-2

Cited by 7 publications

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“…human milk 10 , GC-MS analysis in Indian rivers 11 , dentifrices 12 hydrogel patches 13 , and Dioxin 14 photoproducts has been developed. Some combination analytical methods developed for triclocarban (TCC), triclosan (TCS) and Methyltriclosan (MTCS) in environmental water, by using silicon dioxide/polystyrene composite microspheres solid-phase extraction and detection with HPLC-ESI-MS method 14 .…”

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2014

IJPSR

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The present study is a simple rapid, sensitive, selective and reproducible RP-HPLC method has been developed for the estimation of Triclosan in dental formulations. In present work a simple, sensitive and specific method (RP-HPLC assay, stability indicating RP-HPLC) has been developed by using a phenomenex, BDS C18, column having 5 µm particle size and 150 mm × 4.6 mm in length and gradient mode, with mobile phase containing acetic acid buffer (pH 2.5, adjusted with formic acid) and acetonitrile in the ratio of 30:70. The flow rate was 1.2 mL/min and effluents were monitored by PDA detector at 281nm. Injection volume was 20 µL. The method is linear (1-50 µg/mL). The regression equation was y = 7311x-2307 (r 2 = 0.997). The method was validated for linearity, precision, accuracy, ruggedness, and forced degradation studies were performed. Estimation of triclosan was done in 3 dental formulations. Recoveries from formulations were between 98% and 102%. Limit of detection and limit of quantification was 0.101433 and 0.3333µg/mL, respectively. All the validation parameters are within the acceptance range. INTRODUCTION: Triclosan (5-chloro-2-(2,4dichlorophenoxy)phenol) Fig. 1 is a strong broadspectrum anti-microbial agent used in many consumer products, such as anti-bacterial soaps, toothpaste, cosmetics, clothing, kitchenware, and toys 1. Triclosan is a relatively small molecule , with a molecular weight of 289.54 g/mol and a diameter of about 7.4 Å 2. It is a white solid at standard temperature and pressure, with a boiling point in the range of 280-290 °C and a melting point in the range of 56-58 °C. Triclosan has a low partition coefficient (log Po/w= 4.7) 3. The partition coefficient is a ratio of solubility between two liquids, typically octanol and water.

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2014

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Determination of triclosan in foodstuffs

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A reverse-phase high-performance liquid chromatographic (RP-HPLC) method coupled with an ultraviolet detector was developed to determine triclosan which had migrated into foodstuffs from packaging materials. The method includes extraction with hexane, followed by evaporation to dryness and residue re-dissolution in ACN 90%. Chromatographic separation was performed with a Kromasil 100 C18 column (15 cm x 0.4 cm ID, 5 microm particle size) at 30 degrees C and using ACN and water as mobile phases. Regarding recoveries, good results (higher than 83% and lower than 112%) were obtained for the three representative food matrixes selected (orange juice, chicken breast meat, and Gouda cheese).

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