Simultaneous determination of aminoglycosides and colistins in food

Saluti, Giorgio; Diamanti, Irene; Giusepponi, Danilo; Pucciarini, Lucia; Rossi, Rosanna; Moretti, Simone; Sardella, Roccaldo; Galarini, Roberta

doi:10.1016/j.foodchem.2018.05.113

Cited by 36 publications

(32 citation statements)

References 32 publications

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“…The LOD of kanamycin was determined as 3.56 μg/kg while its LOQ value was determined as 11.85 μg/kg. The validation parameters including LOD, LOQ, and recovery values were compatible with other studies [18][19][20][21][22] .…”

Section: Discussionsupporting

confidence: 84%

“…Similarly, Unusan [25] performed an field monitoring study based on an ELISA method specific for aminoglycosides (streptomycin) in Turkey and reported that only one (1.7%) of 60 ultra-heat-treatment milk samples contained aminoglycosides. de Oliveira et al [26] analyzed a total of 299 pasteurized bovine milk samples collected from retail [13] LC-MS Bovine Milk 0.7 1.0 NA Goutalier et al [18] LC-MS/MS Bovine Milk 14 45 78-104 Bousova et al [19] LC-MS Bovine Milk 33 33 92 Saluti et al [20] LC-MS Bovine Milk <11.5 <18.5 81-91 Tao et al [21] LC-MS Bovine Milk 11 36 78-86 Yang et al [22] NA: Not available markets in Brazil by ELISA kit and an LC-APCI-MS/MS QToF method and reported that none of the samples contained streptomycin and dihydrostreptomycin residues. Also, 192 raw milk samples were analyzed in Hebei Province of China for the presence of twenty-eight veterinary drug residues including kanamycin tested by ultraperformance liquid chromatography with tandem mass spectrometry method and they reported none of the milk samples was contaminated with kanamycin [27] .…”

Section: Discussionmentioning

confidence: 99%

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Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

Acaröz¹,

İnce²,

Arslan‐Acaröz³

et al. 2019

Kafkas Univ Vet Fak Derg

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The present study aimed to evaluate the persistence of kanamycin in lactating Anatolian buffalo milk followed by an intramuscular injection of kanamycin. The collection of milk samples was performed twice daily up to the 10th milking followed by kanamycin injection and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was employed for the residue analysis. The detection limit of the method was determined as 3.56 μg/kg. The highest concentrations of kanamycin were determined in the first milking after injection and mean concentration of this milking was found to be as 1473 μg/kg. Kanamycin residue in all buffalo milk samples was lower than the maximum residue limit (150 μg/kg) with the fifth milking. In addition, a monitoring study was conducted to determine whether buffalo milk marketed in Afyonkarahisar pose a public health risk regarding kanamycin residue. The results of the monitoring study showed that one (2%) of the 50 buffalo milk samples contained kanamycin at the concentration of 22.36 μg/kg. In conclusion, this study determined the persistence of kanamycin in Anatolian buffalo milks based on an LC-MS/MS method. In addition, results of the study showed that buffalo milks marketed in Afyonkarahisar Province pose a very low risk regarding kanamycin residue.Bu çalışmada, laktasyondaki Anadolu mandalarına intramüsküler olarak kanamisin enjekte edilmesini takiben sütlerindeki kanamisin kalıntısının belirlenmesi amaçlandı. Süt numunelerinin toplanması, kanamisin enjeksiyonunu takiben günde iki kez 10'uncu sağıma kadar gerçekleştirildi ve kalıntı analizi için likit kromatografisi tandem kütle spektrometresi (LC-MS/MS) kullanıldı. Metodun tespit limiti 3.56 μg/kg olarak belirlendi. En yüksek kanamisin konsantrasyonları enjeksiyondan sonraki ilk sağımda tespit edildi ve sağımdaki ortalama konsantrasyon 1473 μg/kg olarak belirlendi. Manda sütü örneklerinde kanamisin kalıntı düzeyinin beşinci sağımdan itibaren maksimum kalıntı sınırından (150 μg/kg) daha düşük olduğu belirlendi. Ayrıca, Afyonkarahisar'da pazarlanan manda sütlerinin kanamisin kalıntısı konusunda halk sağlığı açısından risk oluşturup oluşturmadığını belirlemek amacıyla bir saha taraması yapıldı. Saha taraması sonucunda 50 manda sütü örneğinden birinin (%2) 22.36 μg/kg konsantrasyonunda kanamisin içerdiği saptandı. Sonuç olarak bu çalışmada kanamisinin Anadolu manda sütlerinde kalıcılığı LC-MS/MS metodu ile belirlendi. Ayrıca, çalışmanın sonuçları Afyonkarahisar'da tüketime sunulan manda sütlerinin kanamisin kalıntısı açından çok düşük risk taşıdığını gösterdi.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

Acaröz¹,

İnce²,

Arslan‐Acaröz³

et al. 2019

Kafkas Univ Vet Fak Derg

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“…Saluti et al [ 95 ] created a novel system for quantification as well as identification of twelve aminoglycosides (AGs) and two colistins in bovine meat and milk through liquid chromatography combined with quadrupole-orbitrap mass spectrometry and hydrophilic interaction liquid chromatography (HILIC). In HILIC, bare silica poroshell 120 showed the optimum result and the recoveries of all the drugs were near 72–87% in meat (except colistins) and 82–96% in milk.…”

Section: Routine Methods For Colistin Detection In Animals and Itsmentioning

confidence: 99%

Understanding of Colistin Usage in Food Animals and Available Detection Techniques: A Review

Kumar

Chen

Kuča

et al. 2020

Animals

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Progress in the medical profession is determined by the achievements and effectiveness of new antibiotics in the treatment of microbial infections. However, the development of multiple-drug resistance in numerous bacteria, especially Gram-negative bacteria, has limited the treatment options. Due to this resistance, the resurgence of cyclic polypeptide drugs like colistin remains the only option. The drug, colistin, is a well-known growth inhibitor of Gram-negative bacteria like Acinetobacter baumanni, Enterobacter cloacae, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Technological advancements have uncovered the role of the mcr-1(mobilized colistin resistance) gene, which is responsible for the development of resistance in Gram-negative bacteria, which make them distinct from other bacteria without this gene. Additionally, food animals have been determined to be the reservoir for colistin resistance microbes, from which they spread to other hosts. Due to the adverse effects of colistin, many developed countries have prohibited its usage in animal foods, but developing countries are still using colistin in animal food production, thereby imposing a major risk to the public health. Therefore, there is a need for implementation of sustainable measures in livestock farms to prevent microbial infection. This review highlights the negative effects (increased resistance) of colistin consumption and emphasizes the different approaches used for detecting colistin in animal-based foods as well as the challenges associated with its detection.

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“…Xu et al [ 10 ] published a method for colistin A and B in fishery products, and Zhang et al [ 11 ] developed a method for the determination of bacitracin and polymyxin B in livestock products. Recently, two methods were developed for the determination of colistin A and B in animal tissues [ 12 , 13 ]. Meanwhile, there are only a few multi-residue methods for the determination of polypeptide antibiotics in animal matrices.…”

Section: Introductionmentioning

confidence: 99%

Determination of Polypeptide Antibiotic Residues in Food of Animal Origin by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

2020

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A novel UHPLC-MS/MS method for the determination of polypeptide antibiotic residues in animal muscle, milk, and eggs was developed and validated. Bacitracin A, colistin A, colistin B, polymyxin B1, and polymyxin B2 were extracted from the samples with a mixture of acetonitrile/water/ammonia solution 25%, 80/10/10 (v/v/v), and put through further evaporation, reconstitution, and filtration steps. The chromatographic separation was performed on a C18 column in gradient elution mode. Mass spectral acquisitions were performed in selective multiple reaction monitoring mode by a triple quadrupole mass spectrometer. The method was validated according to the criteria of Commission Decision 2002/657/EC. The method quantifies polypeptides in a linear range from 10 to 1000 μg kg−1, where the lowest concentration on the calibration curve refers to the limit of quantification (LOQ). The recoveries ranged from 70 to 99%, the repeatability was below 13%, and within-laboratory reproducibility was lower than 15%. The decision limit (CCα) and detection capability (CCβ) values were calculated, and ruggedness and stability studies were performed, to fulfill the criteria for confirmatory methods. Moreover, the developed method may also be used for screening purposes by its labor efficiency.

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Simultaneous determination of aminoglycosides and colistins in food

Cited by 36 publications

References 32 publications

Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

Understanding of Colistin Usage in Food Animals and Available Detection Techniques: A Review

Determination of Polypeptide Antibiotic Residues in Food of Animal Origin by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

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