Simultaneous Determination of Ambroxol, Guaiphenesin and Levosalbutamol Sulphate in Pharmaceutical Formulations With the Use of Four Rapid Derivative Spectrophotometric Methods

Abstract

Four simple, rapid, accurate, precise, reliable and economical spectrophotometric methods have been proposed for simultaneous determination of ambroxol, guaiphenesin and levosalbutamol sulphate, in pure and pharmaceutical formulations without any prior separation or purification step. The methods are first derivative zero crossing spectrophotometry, simultaneous equation, derivative ratio spectra zero crossing and double divisor ratio spectra derivative method. Developed methods show best results in terms of linearity, accuracy, precision, LOD and LOQ for standard laboratory mixtures of pure drugs and marketed formulations. Common excipients and additives did not interfere in determinations of these APIs. Results obtained by the proposed methods have been statistically compared by means of student t-test.

Simultaneous Estimation of Protein and Nucleic Acid Using Derivative Spectrophotometric Method

“…Under computercontrolled instrumentation, derivative spectrophotometry plays a very important role in the multi-component analysis of mixtures. 8,9 Binary mixtures can be easily resolved by means of a spectrophotometric method, which is based on the simultaneous use of "zero crossing" and "ratio spectra derivative" methods. 10,11 The main aim of the present research work was to investigate the utility of derivative spectrophotometry and to develop reliable spectrophotometric procedure for the simultaneous estimation of protein and nucleic acid either in laboratory samples, or in dosage forms without prior separation of individual components.…”