Simultaneous determination of active flavonoids and alkaloids of Tang-Min-Ling-Pill in rat plasma by liquid chromatography tandem mass spectrometry

Yu, Zhu; Tong, Ling; Zhou, Shuiping; Sun, He; Bi, Kaishun; Zhang, Boli

doi:10.1016/j.jchromb.2012.07.010

Cited by 20 publications

(8 citation statements)

References 21 publications

(22 reference statements)

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“…As shown in Table 3 , two flavonoids showed low AUC and C max in plasma due to their relative low levels in the crude drug and partly forming mono-glucuronide metabolites in plasma [ 19 ]. Chrysin exhibited rapid absorption and a bimodal phenomenon in plasma concentration—time profiles, which was consistent with the previous studies [ 21 , 22 ]. The first peak occurred at about 0.25 h and the second at 8 h due to the glucuronidation [ 19 ] and enterohepatic circulation [ 23 ].…”

Section: Resultssupporting

confidence: 93%

Simultaneous Determination of Chrysin and Tectochrysin from Alpinia oxyphylla Fruits by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study in Normal and Dementia Rats

Zhao

Liu

et al. 2018

Molecules

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A simple and rapid ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) coupled with a one-step liquid-liquid extraction method has been developed and validated for the simultaneous determination of two flavonoids (chrysin, tectochrysin) from Alpinia oxyphylla fruits extract in rat plasma. Plasma samples were extracted with diethyl ether and separated on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) using gradient elution consisting of 0.1% formic acid in water (A) and methanol (B). The multiple reaction monitoring (MRM) mode with electrospray ionization in the positive ion mode was used for detection. The linear range was 0.1 ng/mL to 50 ng/mL for chrysin and tectochrysin, respectively. The accuracy (relative error, RE%) ranged from −8.8% to 7.5% and the intra-day and inter-day precision were within 15% and had a mean extraction recovery rate of 80.3% to 86.7%. The validated method was applied to a comparative pharmacokinetic study after oral administration of Alpinia oxyphylla fruit ethanol extract between normal rats and dementia rats. The area under the curve (AUC) and peak plasma concentration (Cmax) of the two constituents were remarkably increased in dementia rats than in normal rats.

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Section: Resultssupporting

confidence: 93%

Simultaneous Determination of Chrysin and Tectochrysin from Alpinia oxyphylla Fruits by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study in Normal and Dementia Rats

Zhao

Liu

et al. 2018

Molecules

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“…Previous study showed that the alkaloid content of C. yanhusuo was higher with vinegar in its decoction process than that without it [ 4 ]. TCM is often composed of two or more herbs to obtain synergistic effects or to reduce possible adverse reactions [ 5 , 6 , 7 ]. Clinical practice and previous pharmacological studies have proven that Radix Angelicae dahuricae could enhance the analgesic effect of C. yanhusuo [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

An Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Determination of 10 Alkaloids in Beagle Dog Plasma after the Oral Administration of the Corydalis yanhusuo W.T. Wang Extract and Yuanhuzhitong Tablets

et al. 2018

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This study has developed a sensitive and simple ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method for the simultaneous determination of corydaline, dehydrocorydaline, tetrahydropalmatine, protopine, palmatine, tetrahydroberberine, columbamine, berberine, coptisine and berberrubine in beagle dog plasma after the oral administration of the Corydalis yanhusuo W.T. Wang and Yuanhuzhitong tablets. Chromatographic separation was achieved on an Agilent Eclipse Plus C18 RRHD column (1.8 µm, 50 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.3 mL/min. A tandem mass spectrometric detection was conducted by multiple reaction monitoring (MRM) mode via an electrospray ionization source in the positive mode. The calibration curves of all analytes showed good linear (r2 > 0.9800). The intra-day and inter-day precisions were less than 15% and the accuracies were within ±15%. The extraction recoveries conformed to the acceptable range. And there was no interference of endogenous substances in the sensitive assay method. All analytes were proven to be stable during sample storage and analysis procedures. The pharmacokinetic study indicated that the Yuanhuzhitong tablets could get a better absorption than Corydalis yanhusuo W.T. Wang.

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“…The efficiency of a separation can be improved by adjusting the pH, that is, acidifying or basifying the plasma may make ionic target compounds more hydrophobic by ionic suppression so that the analytes move to the organic phase, thereby increasing their recovery (Zhang et al ., ). For instance, extraction in acidic conditions would be favourable for flavonoids because they become less hydrophilic and thus will concentrate in the organic layer (Miniati, ; Zhu et al ., ).…”

Section: Plasma Samplesmentioning

confidence: 99%

“…The criteria for the adoption of one solvent are usually the purity of the organic layer and the recovery (Zheng et al ., ; Zhu et al ., ; Wei et al ., ; Yun et al ., ). Acetone was selected among diethyl ether, methyl tert‐butyl ether and ethyl acetate as the most efficient for the simultaneous extraction of four alkaloids and five flavonoids from rat plasma after the administration of the phytomedicine Tang‐Min‐Ling‐Pill composed of Scutellariae Radix, Coptidis Rhizoma, Aurantii Immaturus Fructus, Paeoniae Alba Radix and Rhei Rhizoma (Table ) (Zhu et al ., ).…”

Section: Plasma Samplesmentioning

confidence: 99%

Sample Preparation for Liquid Chromatographic Analysis of Phytochemicals in Biological Fluids

Lee

2013

Phytochemical Analysis

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Sample treatment for phytochemical analysis in biological fluids is an indispensable and critical step to obtain high quality results. This step could dominate the overall analytical process because both the duration of the process as well as the reliability of the data depend in large part on its efficiency. Thus, special attention should be given to the choice of a proper sample treatment method that targets analytes and their biomatrix.

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Simultaneous determination of active flavonoids and alkaloids of Tang-Min-Ling-Pill in rat plasma by liquid chromatography tandem mass spectrometry

Cited by 20 publications

References 21 publications

Simultaneous Determination of Chrysin and Tectochrysin from Alpinia oxyphylla Fruits by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study in Normal and Dementia Rats

Simultaneous Determination of Chrysin and Tectochrysin from Alpinia oxyphylla Fruits by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study in Normal and Dementia Rats

An Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Determination of 10 Alkaloids in Beagle Dog Plasma after the Oral Administration of the Corydalis yanhusuo W.T. Wang Extract and Yuanhuzhitong Tablets

Sample Preparation for Liquid Chromatographic Analysis of Phytochemicals in Biological Fluids

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