An Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Determination of 10 Alkaloids in Beagle Dog Plasma after the Oral Administration of the Corydalis yanhusuo W.T. Wang Extract and Yuanhuzhitong Tablets

Cui, Binbin; Yang, Jing; Wang, Zhibin; Wu, Chengcui; Dong, Hongrui; Ren, Yixuan; Yang, Chunjuan

doi:10.3390/molecules23081925

Cited by 15 publications

(9 citation statements)

References 25 publications

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“…The retro‐Diels–Alder (RDA) reaction is a characteristic fragmentation pathway of tetrahydroprotoberberine alkaloids 18,21,22 . As an example, corydaline was identified by comparison of the retention time and MS/MS data of the corresponding standard and previous literature 23,24 . The proposed RDA pathway is shown in Figure 3B .…”

Section: Resultsmentioning

confidence: 99%

An integrated strategy for the establishment of a protoberberine alkaloid profile: Exploration of the differences in composition between Tinosporae radix and Fibraurea caulis

Zhao

Zhang

Wang³

et al. 2021

Phytochemical Analysis

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Introduction Accurate species and content identification of major active components in herbals are the guarantee of the safety and effectiveness for medical and commodity purposes. Objectives In this study, an integrated strategy used to establish the protoberberine alkaloid profile was applied to explore the differences in composition between the pieces of Tinosporae radix and Fibraurea caulis, both of which had morphological similarities. Materials and methods First, an in‐house library including possible protoberberine alkaloids based on different substituents was predicted by systematic literature survey. Meanwhile, diagnostic fragments of protoberberine alkaloids were investigated using the corresponding standards. Second, ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UPLC‐QTOF‐MS/MS) was used to obtain multidimensional mass spectral data. Then, the identifications were confirmed by targeted filter of the acquired data based on the library. Results As a result, 10 protoberberine alkaloid molecules including 46 isomers were identified or characterised. The qualitative distribution and relative content of protoberberine alkaloids revealed the fundamental difference between Tinosporae radix and Fibraurea caulis. 25 alkaloids were present in both herbals, while five compounds were detected only in Tinosporae radix. Furthermore, the contents of four alkaloids in Tinosporae radix were significantly higher than those in its adulterant, Fibraurea caulis. Conclusion The five unique ingredients in Tinosporae radix can be used as a better indicator for distinguishing the pieces of Tinosporae radix and Fibraurea caulis. The protoberberine alkaloid profile established in this study can be applied to quality evaluation of the two herbals or other herbals containing major protoberberine alkaloids.

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Section: Resultsmentioning

confidence: 99%

An integrated strategy for the establishment of a protoberberine alkaloid profile: Exploration of the differences in composition between Tinosporae radix and Fibraurea caulis

Zhao

Zhang

Wang³

et al. 2021

Phytochemical Analysis

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“…According to the theory of TCM processing, the toxicity of drugs is reduced and the effects of moving Qi and relieving pain are enhanced by stirfrying with vinegar. Modern research showed that the seven free alkaloid compounds such as protopine, palmatine chloride, berberine, dehydrocorydaline, tetrahydropalmatine, tetrahydroberberine, and corydaline [3][4][5][6][7][8][9] in CR are the main active and characteristic components, which are often used to evaluate the quality of CR. Modern pharmacological studies have indicated that the alkaloid compounds in CR have sedative, analgesic, antitumor, and other pharmacological effects [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Rapid Quality Identification of Decoction Pieces of Crude and Processed Corydalis Rhizoma by Near-Infrared Spectroscopy Coupled with Chemometrics

Zhu

Hong

et al. 2021

Journal of Analytical Methods in Chemistry

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In order to identify the quality of crude and processed Corydalis Rhizoma decoction pieces, the research established a simple, fast, reliable, and validated near-infrared qualitative and quantitative model combined with chemometrics. 51 batches of crude and 40 batches of processed Corydalis Rhizoma from the Zhejiang and Jiangsu provinces of China were collected and analyzed. Crude and processed Corydalis Rhizoma samples were crushed to obtain NIR spectra. The content of seven alkaloids in crude and processed Corydalis Rhizoma was determined by high-performance liquid chromatography (HPLC). Pretreatment methods were screened such as normalization methods, offset filtering methods, and smoothing. Combined with partial least squares-discriminant analysis (PLS-DA) and partial least squares (PLS), the qualitative and quantitative models of crude and processed Corydalis Rhizoma were established, and the correlation coefficient (R2), root mean square error of calibration (RMSEC), and root mean square error of prediction (RMSEP) were used as evaluation indexes. Tetrahydropalmatine was used as an example for screening pretreatment methods; the results showed that MSC combined with the second derivative and no smoothing and the model with the wavelength range of 10000–5000 cm−1 had the best predictive ability and applied to all seven alkaloid components. Among them, the correlation coefficients were all higher than 0.99, and RMSEC and RMSEP were all less than 1%. The qualitative and quantitative model of the seven alkaloids in Corydalis Rhizoma can effectively identify the crude and processed Corydalis Rhizoma and determine the content of the seven alkaloids. By studying the NIR qualitative and quantitative models of crude and processed Corydalis Rhizoma, we can achieve rapid discrimination and quantitative prediction of crude and processed Corydalis Rhizoma. These methods can greatly improve the efficiency of traditional Chinese medicine analysis and provide a strong scientific basis for the quality identification and control of traditional Chinese medicine.

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“…As a classical CPM, this formulation has been commonly used for treating all kinds of pains, such as headache, gastralgia, dysmenorrhea, and costalgia (Li et al, 2018;Wang et al, 2019). YHS is the main constituent herb in YHZT, and according to previous pharmacological studies, alkaloids have been proven to be the major bioactive ingredients in YHS (Cui et al, 2018;Guo et al, 2019;Zhang et al, 2016). For instance, tetrahydropalmatine (THP) is a crucial alkaloid isolated from YHS (Ou et al, 2006) and has been selected as a quantitative marker for quality control of both YHS and YHZT in the ChP 2020 Edition Volume I (Chinese Pharmacopoeia Commission, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…However, previous studies demonstrated that two enantiomers usually behave differently in terms of pharmacodynamic and pharmacokinetic properties (Campo, Bernardes, & Carvalho, 2009;Zhang et al, 2012), but studies on ChP or other studies of quality analysis were generally performed on racemic THP (Cui et al, 2018;Ping et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A new strategy for quality evaluation and control of Chinese patent medicine based on chiral isomer ratio analysis: With Yuanhuzhitong tablet as an example

Gou

Geng

Liu

et al. 2021

Biomedical Chromatography

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Chiral compounds commonly exist in traditional Chinese medicine (TCM), but little research on the quality control of TCM has been conducted. In this study, a new strategy is proposed, taking Yuanhuzhitong tablet [YHZT, consisting of Radix Angelicae Dahuricae and Rhizoma Corydalis (Yan Hu Suo, YHS)] for example, which is based on chiral isomer ratio analysis to monitor the production process of Chinese patent medicine companies. In the process of content determination for tetrahydropalmatine (THP) in YHZT from different companies, noticeable differences were observed in their chromatographic behaviors. It is known that THP has two enantiomers, naturally coexisting in YHS as a racemic mixture, so we prepared THP twice and subsequently performed chiral separation analysis using supercritical fluid chromatography. As a result, the peak area ratios of two enantiomers from different companies varied remarkably, demonstrating that some companies did not probably manufacture YHZT products in accordance with the prescription proportion, used inferior or extracted YSH crude materials in the production process, and added raw chemical medicine in the production to reach the standard and lower the costs. In conclusion, the peak area ratio of chiral isomers could be taken as a key quality index.

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An Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Determination of 10 Alkaloids in Beagle Dog Plasma after the Oral Administration of the Corydalis yanhusuo W.T. Wang Extract and Yuanhuzhitong Tablets

Cited by 15 publications

References 25 publications

An integrated strategy for the establishment of a protoberberine alkaloid profile: Exploration of the differences in composition between Tinosporae radix and Fibraurea caulis

An integrated strategy for the establishment of a protoberberine alkaloid profile: Exploration of the differences in composition between Tinosporae radix and Fibraurea caulis

Rapid Quality Identification of Decoction Pieces of Crude and Processed Corydalis Rhizoma by Near-Infrared Spectroscopy Coupled with Chemometrics

A new strategy for quality evaluation and control of Chinese patent medicine based on chiral isomer ratio analysis: With Yuanhuzhitong tablet as an example

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