Simultaneous determination of acetaminophen, propyphenazone and caffeine in cefalgin preparation by pressurized planar electrochromatography and high-performance thin-layer chromatography

Hałka-Grysińska, Aneta; Ślązak, Piotr; Zaręba, Grzegorz; Markowski, W.; Klimek-Turek, Anna; Dzido, Tadeusz H.

doi:10.1039/c2ay05679h

Cited by 32 publications

(28 citation statements)

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“…Optimization of separation conditions of the components of the cefalgin preparation (propyphenazone, caffeine and acetaminophen) was performed based on our previously presented results [13]. For separation of substance zones of acetaminophen and acetanilide (internal standard), the electrochromatographic system was slightly modified.…”

Section: Resultsmentioning

confidence: 99%

“…Despite these advantages, the potential of this method is not currently used in routine analysis, inter alia due to difficulties with the two stages of the PPEC process that precede the development of electrochromatograms: application of samples on the chromatographic plate and prewetting of an adsorbent layer of the chromatographic plate with a mobile phase solution. Our group has designed pre-wetting reservoirs with partitions, which protected the substance zone applied on the adsorbent layer against elution and dispersion during the prewetting process [6, 10, 11, 13]. Disadvantages of this approach are the risk of losing some of the sample and the need for too many manual operations [13].…”

Section: Introductionmentioning

confidence: 99%

“…Our group has designed pre-wetting reservoirs with partitions, which protected the substance zone applied on the adsorbent layer against elution and dispersion during the prewetting process [6, 10, 11, 13]. Disadvantages of this approach are the risk of losing some of the sample and the need for too many manual operations [13]. The last device proposed by our group was equipped with two partitions in the PPEC chamber, which enabled prewetting of the adsorbent layer with samples zones on it and the separation process in a single unit [14].…”

Section: Introductionmentioning

confidence: 99%

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A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

et al. 2013

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Pressurized planar electrochromatography (PPEC) is a separating technique in which an electric field is applied to force the mobile phase movement through a porous media (electroosmotic effect). High separation efficiency, fast separations and changes in separation selectivity in comparison to liquid chromatography, especially thin layer chromatography (planar chromatography, TLC), are features of this technique. Constructional methodological challenges to PPEC are obstacles to its development and application in laboratory practice. In this article, an attempt to overcome the challenges related to device construction and sample application/injection is described. The introduced device enables both prewetting of the adsorbent layer and electrochromatogram development with a single PPEC device. It also enables simultaneous application/injection of six samples on a chromatographic plate in a stream of the mobile phase (on-line application/injection). In addition, the PPEC chamber was equipped with a thermostat. The device is characterized by an impressive throughput in comparison to the other planar technique, TLC/HPTLC. Although the developed device still needs improvement, it is, in our opinion, a considerable step toward possible automation of this planar separation technique.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

et al. 2013

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“…9,10 A combinação dos três princípios ativos (PRO, PAR e CAF) gera uma atividade terapêutica mais eficiente devido a atuação de cada droga em sítios ativos diferentes no organismo proporcionando maior eficácia analgésica e maior rapidez no início de ação quando compara-se com as drogas administradas separadamente. 11,12 Assim, é possível concluir que o uso de formulações farmacêuticas contendo mais de um princípio ativo apresenta vantagens. Dessa forma, no controle de qualidade deste tipo de medicamento, o uso de metodologias analíticas que permitem determinações simultâneas é altamente desejável.…”

Section: Introductionunclassified

“…Alguns métodos para a determinação simultânea de PAR, PRO e CAF foram localizados na literatura. Os procedimentos empregados anteriormente para este fim foram a cromatografia líquida de alta eficiência, 2,10,13,14 espectrofotometria UV-Vis, [15][16][17][18] cromatografia capilar eletrocinética micelar, 19 eletrocromatografia planar pressurizada, 20 cromatografia de camada delgada de alto desempenho 20 e voltametria de onda quadrada. 21 O sistema conhecido como análise por injeção em batelada com detecção amperométrica de múltiplos pulsos (BIA-MPA, do inglês "Batch Injection Analysis with Multiple Pulse Amperometric detection") vem sendo usado com sucesso em determinações simultâneas de dois 22,23 ou três 24,25 compostos.…”

Section: Introductionunclassified

Determinação Rápida E Simultânea De Propifenazona, Paracetamol E Cafeína Utilizando Análise Por Injeção Em Batelada Com Detecção Amperométrica

Silva¹,

Silva²,

Muñoz³

et al. 2017

Quim. Nova

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FAST SIMULTANEOUS DETERMINATION OF PROPIFENAZONE, PARACETAMOL AND CAFFEINE USING BATCH INJECTION ANALYSIS WITH AMPEROMETRIC DETECTION. A fast and simple procedure for simultaneous determination of propyphenazone (PRO), paracetamol (PAR) and caffeine (CAF) was performed using batch injection analysis with multiple pulse amperometric detection (BIA-MPA). Three potentials pulses were selected for the analysis: +0.90 V/50 ms: only PAR was oxidized; +1.20 V/50 ms: oxidation of PAR and PRO without the interference of CAF; +1.60 V/50 ms: oxidation of the three compounds (PAR, PRO and CAF). The sample aliquot (150 μL) was directly injected onto the boron-doped diamond (BDD) electrode immersed in a BIA cell. The analytical characteristics of the proposed method include high analytical frequency (75 injections per hour), good stability (RSD < 3.9%; n = 10), and low detection limits (0.3, 0.2 and 0.1 mg L −1 for PRO, PAR and CAF, respectively). The proposed method yielded similar results to those obtained by liquid chromatography at a 95% confidence level.

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High‐Performance Thin‐Layer Chromatography

Cebolla

Membrado

Jarne

et al. 2015

Analytical Separation Science

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The instrumental modularity of its basic steps, that is, sample application, chromatographic development, and detection, confers HPTLC a high degree of flexibility. Automated and computer‐controlled instruments with different levels of sophistication for each of these steps have been developed, for example, spray‐on sample applicators and chambers including the possibility of gradient separation. Detection techniques such as scanning densitometers, electronic documentation systems, bioactivity detection methodologies, and online coupling with mass spectrometry have come to be easily and firmly established in laboratories. Particularly, the introduction of an elution‐based TLC – MS interface has not only allowed original and efficient hyphenated systems to be designed but also opened the possibility of expanding the connection with other detectors. Thanks to this instrumental development, HPTLC has become a reliable, accurate, and sensitive analytical technique.

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Simultaneous determination of acetaminophen, propyphenazone and caffeine in cefalgin preparation by pressurized planar electrochromatography and high-performance thin-layer chromatography

Cited by 32 publications

References 23 publications

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

Determinação Rápida E Simultânea De Propifenazona, Paracetamol E Cafeína Utilizando Análise Por Injeção Em Batelada Com Detecção Amperométrica

High‐Performance Thin‐Layer Chromatography

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