Simultaneous determination of 8 HIV protease inhibitors in human plasma by isocratic high-performance liquid chromatography with combined use of UV and fluorescence detection: Amprenavir, indinavir, atazanavir, ritonavir, lopinavir, saquinavir, nelfinavir and M8-nelfinavir metabolite

Comparison of extraction procedures for assessment of matrix effect for selective and reliable determination of atazanavir in human plasma by LC–ESI-MS/MS

“…ATV is determined in peripheral blood mononuclear cells (PBMC) [28,31,47,48,51], cerebrospinal fluid and seminal plasma [31], serum [41], dried blood spots [42] and human plasma [24][25][26][27]29,30,[32][33][34][35][36][37][38][39][40][43][44][45][46]49,50]. As a single analyte, ATV has been determined in PBMC [18] and human plasma [19][20][21][22][23][24][25].…”

Reversed-phase liquid chromatographic method with spectrophotometric detection for the determination of antiretroviral drugs

“…The implementation of TDM for HIV treatment has led to a proliferation of analytical methods for quantifying PI [7,8], NNRTI [9,10] and NRTI [11][12][13][14] drugs and combinations of families [15][16][17][18][19]. Most of these methods concern the analysis of plasma although other body fluids such as urine, semen and amniotic liquid have also been analyzed [6,7,20,21].…”

“…Cleanup procedures are commonly required for sample treatment to avoid interferences from multiple plasma components, which may overlap analyte peaks, and matrix effects affecting the sensitivity of the calibration curve [8]. Solid-phase extraction (SPE) using commercial cartridges is often used for the analysis of NRTIs [6,9,10,12,13,[19][20][21], while liquid-liquid extraction has widely been applied to NNRTI and PI drugs [7,9,[14][15][16][17][18].…”

Quantification of 8 HIV-Protease Inhibitors and 2 Nonnucleoside Reverse Transcriptase Inhibitors by Ultra-Performance Liquid Chromatography with Diode Array Detection

“…Furthermore, UV detection remains an available option for many laboratories. Unfortunately, analysis times are usually very long with conventional HPLC columns (up to 70 min) (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) because UV detection requires complete resolution of all chromatographic peaks to avoid interferences. Faster and higher-resolution separation can be achieved with ultra-performance liquid chromatography (UPLC) technology.…”

“…We did not test the stability of the ARV drugs in plasma and methanol, because stability assessments for the drugs we tested have largely been performed in other studies (3,4,6,(13)(14)(15), and these studies have shown the drugs to be stable, even after heat inactivation of the virus.…”