Simultaneous determination of 3-nitrotyrosine, tyrosine, hydroxyproline and proline in exhaled breath condensate by hydrophilic interaction liquid chromatography/electrospray ionization tandem mass spectrometry

Conventz, Angela; Musiol, A.; Brodowsky, C; Müller-Lux, A.; Dewes, P.; Kraus, Thomas; Schettgen, Thomas

doi:10.1016/j.jchromb.2007.10.031

Cited by 55 publications

(28 citation statements)

References 39 publications

(60 reference statements)

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“…Gessner et al (Gessner et al 2001) pointed out that proteins may be a suitable reference substance to correct for dilution eVects. However, according to our previous investigations (Conventz et al 2007), non-volatile substances like amino acids are well suited to correct for the degree of dilution of the lining Xuid in the collected EBCs. Consequently, we chose to determine the amino acids tyrosine and proline (which are the biochemical precursors of 3-nitrotyrosine and trans-hydroxyproline) as "internal standards" to normalize for all measured biomarker concentrations.…”

Section: Discussionmentioning

confidence: 99%

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Biological effect markers in exhaled breath condensate and biomonitoring in welders: impact of smoking and protection equipment

Gube

Ebel

Brand

et al. 2010

Int Arch Occup Environ Health

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Section: Discussionmentioning

confidence: 99%

“…Simultaneous measurement of 3-nitrotyrosine, tyrosine, hydroxyproline and proline in EBC was undertaken according to our previous publication (Conventz et al 2007). In brief, 1 ml of EBC was freeze-dried and reconstituted in 50 l of HILIC eluent for concentration purposes.…”

Section: Chemical Analysis Of Biomarkers In Ebcmentioning

confidence: 99%

Biological effect markers in exhaled breath condensate and biomonitoring in welders: impact of smoking and protection equipment

Gube

Ebel

Brand

et al. 2010

Int Arch Occup Environ Health

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“…The use of metabolomics, proteomics and new modes of spectrometry further facilitate exhaled biomarker research [101][102][103][104][105][106][107][108][109][110][111][112][113]. Metabolomic pattern determined by using nuclear magnetic resonance measurements in EBC has been shown to discriminate between healthy and asthmatic children [111].…”

Section: Future Developments In Breath Testingmentioning

confidence: 99%

Exhaled biomarkers in lung cancer

Horváth

Lázár

Gyulai

et al. 2009

European Respiratory Journal

239

206

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Lung cancer is the leading cause of cancer death. Results of therapeutic interventions are particularly discouraging when the disease is discovered in an advanced stage. Early diagnosis is limited by the fact that the disease usually develops asymptomatically and available screening methods do not fulfil the requirements for reliable discrimination between patients with lung cancer and subjects not suffering from the disease. Breath sampling is completely noninvasive and provides a potentially useful approach to screening lung cancer. Exhaled biomarkers contain both volatile and nonvolatile molecules. The profile of volatile organic compounds is different in patients with lung cancer than in control subjects. In exhaled breath condensate, the proteomic profile of breath from cancer patients differs from that of healthy smokers. We reviewed the scientific evidence demonstrating that a unique chemical signature can be detected in the breath of patients with lung cancer and that the exhaled breath biomarker profile could aid clinical decision making.KEYWORDS: Biomarker, electronic nose, exhaled breath, exhaled breath condensate, lung cancer, smell L ung cancer is the leading cause of global cancer death in both males and females. According to the most recent projection of global mortality, by 2030 it will emerge as the third and the fifth leading cause of death in highand middle-income countries, respectively [1,2]. Figures on disease outcome measures are very discouraging as even with the most advanced treatment strategies ,86% of lung cancer patients die within 5 yrs of diagnosis. With early detection and treatment, however, the 5-yr survival rate improves dramatically from 20% in patients with stage III lung cancer to 70% in patients with stage I disease [3]. Researchers, therefore, have sought out screening tests to detect lung cancer in the earliest stages and several promising new approaches have been proposed for this purpose, such as computer-assisted image analysis of chest radiographs, spiral computed tomography (CT) scanning, PCR-based assays of sputum and fluorescence bronchoscopy [4][5][6][7].Breath chemical tests have a broad spectrum of applications ranging from the US Food and Drug Administration-approved exhaled nitric oxide fraction (FeNO) measurement to monitor the effect of anti-inflammatory treatment in asthma, to volatile organic compound (VOC) determination and nonvolatile biomarker profiling in the cooled breath sample called exhaled breath condensate (EBC) [8][9][10][11]. Being completely noninvasive, sampling of the breath allows clinicians and researchers to assess different body functions in a flexible manner. Breath collection can be performed even in very severe patients and also repeated within short intervals. Therefore, breath testing is considered to be a potentially ideal candidate for screening purposes. Besides widely known constituents such as nitrogen, oxygen, carbon dioxide, inert gases and water vapour, exhaled breath also consists of thousands of volatile and nonv...

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“…HILIC mode chromatography is important for the separation of highly polar substances such as un-derivatised amino acids [35][36][37] and the method is widely used in different fields including pharmaceutical, food science, environmental analysis and biomolecular research [38][39][40][41][42]. Moreover, there has been an increasing interest in HILIC since the 1980 s due to the use of low aqueous/high polar organic solvent mobile phase which are particularly suitable for ESI/MS detection as it results in increased sensitivity [43].…”

Section: Instrumental Performancementioning

confidence: 99%

Quantitative determination of un-derivatised amino acids in artistic mural paintings using high-performance liquid chromatography/electrospray ionization triple quadrupole mass spectrometry

Zangrando¹,

Piazza²,

Cairns³

et al. 2010

Analytica Chimica Acta

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Simultaneous determination of 3-nitrotyrosine, tyrosine, hydroxyproline and proline in exhaled breath condensate by hydrophilic interaction liquid chromatography/electrospray ionization tandem mass spectrometry

Cited by 55 publications

References 39 publications

Biological effect markers in exhaled breath condensate and biomonitoring in welders: impact of smoking and protection equipment

Biological effect markers in exhaled breath condensate and biomonitoring in welders: impact of smoking and protection equipment

Exhaled biomarkers in lung cancer

Quantitative determination of un-derivatised amino acids in artistic mural paintings using high-performance liquid chromatography/electrospray ionization triple quadrupole mass spectrometry

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