Simultaneous Determination of 19 Plant Growth Regulator Residues in Plant-originated Foods by QuEChERS and Stable Isotope Dilution-Ultra Performance Liquid Chromatography-Mass Spectrometry

Abstract

Appropriate and fast analytical methods are required for plantoriginated foods, given the great significance therein for food safety, human health and social economic development. In this study, a highly sensitive and fast method of QuEChERS (acronym of quick, easy, cheap, effective, rugged, safe) combined with isotope dilution-ultra performance liquid chromatography coupled to tandem mass spectrometry was developed for the simultaneous determination of 19 plant growth regulators in plant-originated foods. The samples were initially extracted with acetonitrile containing 1% acetic acid and then the QuEChERS method was applied after the pH value was adjusted to 5.5 -6.0, using 3-indolepropionic-d2 acid and forchlorfenuron-d5 as internal standard. The targeted 19 plant growth regulators were separated on an HSS T3 column using acetonitrile: 5 mmol/L ammonium acetate as the mobile phase. Quantitative results were based on multiple reaction monitoring mode after ionization in positive and negative electrospray ionization mode. Good linearity was achieved within a wide range and all the correlation coefficients were greater than 0.997. The limit of quantification was 0.060 -6.0 μg/kg. Rate of recovery and relative standard deviation were 72.3 -115.8 and 1.78 -5.32%, respectively. The method was successfully applied to measure 19 plant growth regulator residues in 280 plant-originated commercial foods collected from local supermarkets in China. Twelve plant growth regulators were found in some of the analyzed samples.

The phytohormone forchlorfenuron decreases viability and proliferation of malignant mesothelioma cells in vitro and in vivo

“…The synthetic urea derivative forchlorfenuron (FCF) is a phytohormone with cytokinin activity and is used worldwide as a plant growth regulator that increases fruit size. Since its development in the 1980s, FCF has been widely used in grapes, kiwifruits, apples, pears and watermelons [16, 17]. In 2004, Iwase et al [18] reported that FCF treatment caused defects in cytokinesis and deformation of septin filaments in budding yeast; the effects are rapid and reversible upon removal of FCF.…”

Development and validation of LC-MS/MS method for simultaneous determination of 6-benzylaminopurine and forchlorfenuron in bean sprout

“…Nhiều phương pháp đã được xây dựng để xác định tồn dư các chất 6-BA và CPPU, trong đó phương pháp ELISA [4], LC-MS/MS kết hợp cùng quy trình xử lý mẫu bằng kỹ thuật SPE [5], QuEChERS [6][7]…”

Gas Chromatography-Mass Spectrometry Method for Simultaneous Detection of Nine Alkaloids in Tobacco and Tobacco Products by QuEChERS Sample Preparation

“…27 Today, it has gradually become a standard sample processing approach achieving great success in detecting pesticide residues in fruits and vegetables. [28][29][30][31][32] The sample was taken out with an organic solvent and subjected to salting and delamination of MgSO4 or additional salts. Based on the principle of matrix dispersion and separation, different kinds of adsorbents were applied to purify and remove impurities, so that less abundant components in the samples were easily measured.…”