Gas Chromatography-Mass Spectrometry Method for Simultaneous Detection of Nine Alkaloids in Tobacco and Tobacco Products by QuEChERS Sample Preparation

Li, Xintong; Liu, Fang; Wang, Hui‐Fang; He, Fan; Yang, Rui; Zhao, Mingqin

doi:10.2116/analsci.19p063

Cited by 13 publications

(8 citation statements)

References 34 publications

(31 reference statements)

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“…In the end, both approaches (direct organic solvent and QuEChERS extraction) were efficient on recovering the targeted compounds as demonstrated by analytical method performance, with QuEChERS protocol, yielding cleaner extracts, therefore it was selected and fully validated. Alkaloids have been adequately extracted using QuEChERS from tea [51], tobacco [52], feed [53] and honey [54]. Therefore, this work contributes to the frontier of alkaloids extraction from another matrix (honeybees).…”

Section: Discussionmentioning

confidence: 99%

An essay on ecosystem availability of Nicotiana glauca graham alkaloids: the honeybees case study

et al. 2020

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Background Invasive plant species pose a significant threat for fragile isolated ecosystems, occupying space, and consuming scarce local resources. Recently though, an additional adverse effect was recognized in the form of its secondary metabolites entering the food chain. The present study is elaborating on this subject with a specific focus on the Nicotiana glauca Graham (Solanaceae) alkaloids and their occurrence and food chain penetrability in Mediterranean ecosystems. For this purpose, a targeted liquid chromatography electrospray tandem mass spectrometric (LC–ESI–MS/MS) analytical method, encompassing six alkaloids and one coumarin derivative, utilizing hydrophilic interaction chromatography (HILIC) was developed and validated. Results The method exhibited satisfactory recoveries, for all analytes, ranging from 75 to 93%, and acceptable repeatability and reproducibility. Four compounds (anabasine, anatabine, nornicotine, and scopoletin) were identified and quantified in 3 N. glauca flowers extracts, establishing them as potential sources of alien bio-molecules. The most abundant constituent was anabasine, determined at 3900 μg/g in the methanolic extract. These extracts were utilized as feeding treatments on Apis mellifera honeybees, resulting in mild toxicity documented by 16–18% mortality. A slightly increased effect was elicited by the methanolic extract containing anabasine at 20 μg/mL, where mortality approached 25%. Dead bees were screened for residues of the N. glauca flower extracts compounds and a significant mean concentration of anabasine was evidenced in both 10 and 20 μg/mL treatments, ranging from 51 to 92 ng/g per bee body weight. Scopoletin was also detected in trace amounts. Conclusions The mild toxicity of the extracts in conjunction with the alkaloid and coumarin residual detection in bees, suggest that these alien bio-molecules are transferred within the food chain, suggesting a chemical invasion phenomenon, never reported before.

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Section: Discussionmentioning

confidence: 99%

An essay on ecosystem availability of Nicotiana glauca graham alkaloids: the honeybees case study

et al. 2020

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“…To determine the nutrient contents in first flue-cured tobacco leaves, 1 kg leave sample was randomly taken in each treatment, in which the main veins were removed, and grinded and sieved through a 0.4 mm sieve before analyzing the chemical components (Sun et al, 2011). The alkaloids in tobacco leaves including nicotine, nornicotine, myosmine, N-methyl anabasine, β-nicotyrine, anabasine, anatabine, isonicotenine and cotinine, were determined by gas chromatography-mass spectrometry method (Li et al, 2019) and their contents were summed to calculate ratio of sugar to alkaloids.…”

Section: Chemical Analysismentioning

confidence: 99%

Long-term organic fertilizer additions elevate soil extracellular enzyme activities and tobacco quality in a tobacco-maize rotation

Jiang

Zhang

et al. 2022

Front. Plant Sci.

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Organic fertilizer is effective in improving soil quality, and promoting crop growth. Combined organic and inorganic fertilization has been proved as a more favorable way to tobacco yield and quality. However, the mechanisms underlying tobacco yield and quality under combinations of different organic and inorganic fertilizer remain unclear. We conducted a 12-year tobacco (Nicotiana tabacum L.)-maize crop rotation field experiment in Yanhe experimental station, China to examine the yields and qualities of tobacco, soil nutrients, and extracellular enzyme activities associated with carbon, nitrogen, and phosphorus cycles in response to different fertilization treatments. Five fertilization treatments (no fertilization; 75 kg N fertilizer ha–1; 450 kg oil cake ha–1 + 75 kg N fertilizer ha–1; 15,000 kg pig dung ha–1 + 60 kg N fertilizer ha–1; 3,000 kg straw ha–1 + 75 kg N fertilizer ha–1) were applied to tobacco while maize was fertilized with inorganic compound fertilizers. After 12 years of tobacco-maize rotation, the results showed that organic fertilizer additions elevate tobacco yield and quality, and the soil extracellular enzymes activities. Gram-negative bacteria, actinomycetes, and total soil microbial biomass were increased by organic fertilizer additions, both plant-based (oil cake and straw) and animal-based (pig dung) organics. The levels of soil organic matter, total organic carbon, total phosphorus and available phosphorus are higher in pig dung addition treatment than oil cake and straw additions. By variance analysis with respect to fertilization treatments, organic sources differentially affected the activities of diverse soil enzymes. The redundancy analysis gave that yield and quality of tobacco leaves (upper, middle, and lower leaves) positively related to soil extracellular enzyme activities. Based on analysis of yield and quality of tobacco leaves with extracellular enzyme activities and soil nutrients, it is suggested animal-based organic fertilizer, thus pig dung, should be used in combining with chemical fertilizers to improve the quality of tobacco and soil nutrients.

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“…Numerous studies have demonstrated that tobacco leaves contain a number of active substances, such as saccharides, organic acids, alkaloids and proteins [3][4][5][6][7]. However, there are few studies on the contents of these substances in waste materials, such as tobacco stems [8].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Ultrasonic-Assisted Extraction of Chlorogenic Acid from Tobacco Waste

Zeng

Ran

Huang

et al. 2022

IJERPH

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Using tobacco waste as raw material, the ultrasonic-assisted extraction of chlorogenic acid was optimized by response surface methodology (RSM). After repeated freezing and thawing of tobacco waste twice, the effect of pH value, ethanol volume fraction, temperature and extraction time on the extraction rate of chlorogenic acid was investigated by a single factor experiment. On the basis of this, the factors affecting the yield of chlorogenic acid were further optimized by using RSM. The optimum extraction conditions for chlorogenic acid were set at pH = 4.1, ethanol volume fraction was 49.57% and extraction time was 2.06 h. Under the above conditions, the extraction rate of chlorogenic acid could reach 0.502%, which was higher than traditional extraction and unpretreated ultrasonic extraction. All these results can be used as a reference for the extraction of effective ingredients in tobacco waste.

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Gas Chromatography-Mass Spectrometry Method for Simultaneous Detection of Nine Alkaloids in Tobacco and Tobacco Products by QuEChERS Sample Preparation

Cited by 13 publications

References 34 publications

An essay on ecosystem availability of Nicotiana glauca graham alkaloids: the honeybees case study

An essay on ecosystem availability of Nicotiana glauca graham alkaloids: the honeybees case study

Long-term organic fertilizer additions elevate soil extracellular enzyme activities and tobacco quality in a tobacco-maize rotation

Optimization of Ultrasonic-Assisted Extraction of Chlorogenic Acid from Tobacco Waste

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