Optimization of Ultrasonic-Assisted Extraction of Chlorogenic Acid from Tobacco Waste

Abstract

Using tobacco waste as raw material, the ultrasonic-assisted extraction of chlorogenic acid was optimized by response surface methodology (RSM). After repeated freezing and thawing of tobacco waste twice, the effect of pH value, ethanol volume fraction, temperature and extraction time on the extraction rate of chlorogenic acid was investigated by a single factor experiment. On the basis of this, the factors affecting the yield of chlorogenic acid were further optimized by using RSM. The optimum extraction conditions for chlorogenic acid were set at pH = 4.1, ethanol volume fraction was 49.57% and extraction time was 2.06 h. Under the above conditions, the extraction rate of chlorogenic acid could reach 0.502%, which was higher than traditional extraction and unpretreated ultrasonic extraction. All these results can be used as a reference for the extraction of effective ingredients in tobacco waste.

High-intensity ultrasound-based process strategies for obtaining edible sunflower (Helianthus annuus L.) flour with low-phenolic and high-protein content

“…According to the literature, increasing ultrasound power favors chlorogenic acid extraction [37] , [38] . However, we observed that prolonged extraction time (30 min) reduces phenolic compound extraction yield, probably due to chlorogenic acid degradation induced by oxidative mechanisms associated with ultrasound action [35] , [39] , [40] , [37] .

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“…The extraction rate of phenolic compounds reached a plateau with increasing extraction time, resulting in increased sample core temperature. The exacerbated increase in sonication temperature can lead to a reduction in the extraction yield of bioactive compounds as they degrade by oxidation mechanisms induced by thermal, mechanical and chemical stresses that occur during acoustic cavitation [35] , [36] .…”

Full‐length transcriptome sequencing and transgenic tobacco revealed the key genes in the chlorogenic acid synthesis pathway of Sambucus chinensis L.

“…Washed roots, stems, leaves, buds and fruits of S. chinensis and leaves of N. tabacum K326 were dried to constant weight in an electric constant temperature blast drying oven at 50°C (Tianjin Taisite Instrument Co. Ltd., 1C1‐2AB). Then, the materials were ground separately and passed through a 40‐mesh sieve (Zeng et al, 2022). CGA from 0.2 g sample powder was extracted by anhydrous ethanol (the ratio of material to liquid 1:40) for 30 min under Ultrasonic conditions at 100 W (Shanghai Gutel Ultrasonic Instrument Co. Ltd., SG3200HPT), then centrifuged at 5500 g for 15 min (Xiangli, TD4Z‐WS).…”

Advances in Production of Hydroxycinnamoyl-Quinic Acids: From Natural Sources to Biotechnology

“…Therefore, SLE is considered the most relevant method for industrial application due to its simplicity, reproducibility, low cost and possibility to use environment friendly solvents [ 84 ]. Intensified methods are mostly applied for the recovery of HCQAs from waste [ 71 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 ] ( Table 4 ).…”